Evolutionary Analysis of Minor Histocompatibility Genes In Hydra

Handle URI:
http://hdl.handle.net/10754/609123
Title:
Evolutionary Analysis of Minor Histocompatibility Genes In Hydra
Authors:
Aalismail, Nojood ( 0000-0001-7268-792X )
Abstract:
Hydra is a simple freshwater solitary polyp used as a model system to study evolutionary aspects. The immune response of this organism has not been studied extensively and the immune response genes have not been identified and characterized. On the other hand, immune response has been investigated and genetic analysis has been initiated in other lower invertebrates. In the present study we took initiative to study the self/nonself recognition in hydra and its relation to the immune response. Moreover, performing phylogenetic analysis to look for annotated immune genes in hydra gave us a potential to analyze the expression of minor histocompatibility genes that have been shown to play a major role in grafting and transplantation in mammals. Here we obtained the cDNA library that shows expression of minor histocompatibility genes and confirmed that the annotated sequences in databases are actually present. In addition, grafting experiments suggested, although still preliminary, that homograft showed less rejection response than in heterograft. Involvement of possible minor histocompatibility gene orthologous in immune response was examined by qPCR.
Advisors:
Gojobori, Takashi ( 0000-0001-7850-1743 )
Committee Member:
Pain, Arnab ( 0000-0002-1755-2819 ) ; Mineta, Katsuhiko ( 0000-0002-4727-045X )
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience
Program:
Bioscience
Issue Date:
May-2016
Type:
Thesis
Appears in Collections:
Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorGojobori, Takashien
dc.contributor.authorAalismail, Nojooden
dc.date.accessioned2016-05-12T06:59:13Zen
dc.date.available2016-05-12T06:59:13Zen
dc.date.issued2016-05en
dc.identifier.urihttp://hdl.handle.net/10754/609123en
dc.description.abstractHydra is a simple freshwater solitary polyp used as a model system to study evolutionary aspects. The immune response of this organism has not been studied extensively and the immune response genes have not been identified and characterized. On the other hand, immune response has been investigated and genetic analysis has been initiated in other lower invertebrates. In the present study we took initiative to study the self/nonself recognition in hydra and its relation to the immune response. Moreover, performing phylogenetic analysis to look for annotated immune genes in hydra gave us a potential to analyze the expression of minor histocompatibility genes that have been shown to play a major role in grafting and transplantation in mammals. Here we obtained the cDNA library that shows expression of minor histocompatibility genes and confirmed that the annotated sequences in databases are actually present. In addition, grafting experiments suggested, although still preliminary, that homograft showed less rejection response than in heterograft. Involvement of possible minor histocompatibility gene orthologous in immune response was examined by qPCR.en
dc.language.isoenen
dc.subjectHydraen
dc.subjectMinor Histocompatibilityen
dc.subjectImmuneen
dc.titleEvolutionary Analysis of Minor Histocompatibility Genes In Hydraen
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscienceen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberPain, Arnaben
dc.contributor.committeememberMineta, Katsuhikoen
thesis.degree.disciplineBioscienceen
thesis.degree.nameMaster of Scienceen
dc.person.id132945en
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