Cell Surface Enzymatic Engineering-Based Approaches to Improve Cellular Therapies

Handle URI:
http://hdl.handle.net/10754/608652
Title:
Cell Surface Enzymatic Engineering-Based Approaches to Improve Cellular Therapies
Authors:
AbuElela, Ayman ( 0000-0002-4529-3156 ) ; Sakashita, Kosuke ( 0000-0002-7118-5511 ) ; Merzaban, Jasmeen S. ( 0000-0002-7276-2907 )
Abstract:
The cell surface represents the interface between the cell and its environment. As such, the cell surface controls cell–cell interactions and functions such as adhesion and migration, and will transfer external cues to regulate processes such as survival, death, and differentiation. Redefining the cell surface by temporarily (or permanently) modifying the molecular landscape of the plasma membrane affects the way in which the cell interacts with its environment and influences the information that is relayed into the cell along downstream signaling pathways. This chapter outlines the role of key enzymes, the glycosyltransferases, in posttranslationally modifying proteins and lipids to fine-tune cells, ability to migrate. These enzymes are critical in controlling the formation of a platform structure, sialyl Lewis x (sLex), on circulating cells that plays a central role in the recognition and recruitment by selectin counter receptors on endothelial cells that line blood vessels of tissues throughout the body. By developing methods to manipulate the activity of these enzymes and hence the cell surface structures that result, treatments can be envisioned that direct the migration of therapeutic cells to specific locations throughout the body and also to inhibit metastasis of detrimental cells such as circulating tumor cells.
KAUST Department:
Biological and Environmental Sciences and Engineering
Publisher:
Elsevier BV
Journal:
Micro- and Nanoengineering of the Cell Surface
Issue Date:
6-Jun-2014
DOI:
10.1016/B978-1-4557-3146-6.00009-X
Type:
Book Chapter
ISBN:
978-1-4557-3146-6
Additional Links:
http://www.sciencedirect.com/science/article/pii/B978145573146600009X
Appears in Collections:
Book Chapters

Full metadata record

DC FieldValue Language
dc.contributor.authorAbuElela, Aymanen
dc.contributor.authorSakashita, Kosukeen
dc.contributor.authorMerzaban, Jasmeen S.en
dc.date.accessioned2016-05-09T08:43:11Zen
dc.date.available2016-05-09T08:43:11Zen
dc.date.issued2014-06-06en
dc.identifier.isbn978-1-4557-3146-6en
dc.identifier.doi10.1016/B978-1-4557-3146-6.00009-Xen
dc.identifier.urihttp://hdl.handle.net/10754/608652en
dc.description.abstractThe cell surface represents the interface between the cell and its environment. As such, the cell surface controls cell–cell interactions and functions such as adhesion and migration, and will transfer external cues to regulate processes such as survival, death, and differentiation. Redefining the cell surface by temporarily (or permanently) modifying the molecular landscape of the plasma membrane affects the way in which the cell interacts with its environment and influences the information that is relayed into the cell along downstream signaling pathways. This chapter outlines the role of key enzymes, the glycosyltransferases, in posttranslationally modifying proteins and lipids to fine-tune cells, ability to migrate. These enzymes are critical in controlling the formation of a platform structure, sialyl Lewis x (sLex), on circulating cells that plays a central role in the recognition and recruitment by selectin counter receptors on endothelial cells that line blood vessels of tissues throughout the body. By developing methods to manipulate the activity of these enzymes and hence the cell surface structures that result, treatments can be envisioned that direct the migration of therapeutic cells to specific locations throughout the body and also to inhibit metastasis of detrimental cells such as circulating tumor cells.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/B978145573146600009Xen
dc.titleCell Surface Enzymatic Engineering-Based Approaches to Improve Cellular Therapiesen
dc.typeBook Chapteren
dc.contributor.departmentBiological and Environmental Sciences and Engineeringen
dc.identifier.journalMicro- and Nanoengineering of the Cell Surfaceen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAbuElela, Aymanen
kaust.authorSakashita, Kosukeen
kaust.authorMerzaban, Jasmeen S.en
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