Please mind the gap – Visual census and cryptic biodiversity assessment at central Red Sea coral reefs

Handle URI:
http://hdl.handle.net/10754/608644
Title:
Please mind the gap – Visual census and cryptic biodiversity assessment at central Red Sea coral reefs
Authors:
Pearman, John K. ( 0000-0002-2237-9723 ) ; Anlauf, Holger; Irigoien, Xabier ( 0000-0002-5411-6741 ) ; Carvalho, Susana ( 0000-0003-1300-1953 )
Abstract:
Coral reefs harbor the most diverse assemblages in the ocean, however, a large proportion of the diversity is cryptic and, therefore, undetected by standard visual census techniques. Cryptic and exposed communities differ considerably in species composition and ecological function. This study compares three different coral reef assessment protocols: i) visual benthic reef surveys: ii) visual census of Autonomous Reef Monitoring Structures (ARMS) plates; and iii) metabarcoding techniques of the ARMS (including sessile, 106–500 μm and 500–2000 μm size fractions), that target the cryptic and exposed communities of three reefs in the central Red Sea. Visual census showed a dominance of Cnidaria (Anthozoa) and Rhodophyta on the reef substrate, while Porifera, Bryozoa and Rhodophyta were the most abundant groups on the ARMS plates. Metabarcoding, targeting the 18S rRNA gene, significantly increased estimates of the species diversity (p < 0.001); revealing that Annelida were generally the dominant phyla (in terms of reads) of all fractions and reefs. Furthermore, metabarcoding detected microbial eukaryotic groups such as Syndiniophyceae, Mamiellophyceae and Bacillariophyceae as relevant components of the sessile fraction. ANOSIM analysis showed that the three reef sites showed no differences based on the visual census data. Metabarcoding showed a higher sensitivity for identifying differences between reef communities at smaller geographic scales than standard visual census techniques as significant differences in the assemblages were observed amongst the reefs. Comparison of the techniques showed no similar patterns for the visual techniques while the metabarcoding of the ARMS showed similar patterns amongst fractions. Establishing ARMS as a standard tool in reef monitoring will not only advance our understanding of local processes and ecological community response to environmental changes, as different faunal components will provide complementary information but also improve the estimates of biodiversity in coral reef benthic communities. This study lays the foundations for further studies looking at integrating traditional reef survey methodologies with complementary approaches, such as metabarcoding, which investigate other components of the reef community.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Please mind the gap – Visual census and cryptic biodiversity assessment at central Red Sea coral reefs 2016, 118:20 Marine Environmental Research
Publisher:
Elsevier BV
Journal:
Marine Environmental Research
Issue Date:
26-Apr-2016
DOI:
10.1016/j.marenvres.2016.04.011
Type:
Article
ISSN:
01411136
Sponsors:
The authors would like to thank the skippers and crew from CMOR, KAUST who enabled the sampling to be performed. The authors are also indebted to the help given during sampling and laboratory work from Eva Aylagas Martinez and Miguel Viegas, as well as Saskia Kürten for undertaking the nutrient analysis. We would also like to thank Ute Langner for the production of the map. Furthermore, the authors would like to acknowledge the assistance provide in the improvement of this manuscript from the two reviewers. The research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST). This study was undertaken within the framework of the project DEVOTES (EU - ENV.2012.6.2–3 - Grant agreement 308392; http://www.devotes-project.eu).
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0141113616300617
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPearman, John K.en
dc.contributor.authorAnlauf, Holgeren
dc.contributor.authorIrigoien, Xabieren
dc.contributor.authorCarvalho, Susanaen
dc.date.accessioned2016-05-09T07:51:37Zen
dc.date.available2016-05-09T07:51:37Zen
dc.date.issued2016-04-26en
dc.identifier.citationPlease mind the gap – Visual census and cryptic biodiversity assessment at central Red Sea coral reefs 2016, 118:20 Marine Environmental Researchen
dc.identifier.issn01411136en
dc.identifier.doi10.1016/j.marenvres.2016.04.011en
dc.identifier.urihttp://hdl.handle.net/10754/608644en
dc.description.abstractCoral reefs harbor the most diverse assemblages in the ocean, however, a large proportion of the diversity is cryptic and, therefore, undetected by standard visual census techniques. Cryptic and exposed communities differ considerably in species composition and ecological function. This study compares three different coral reef assessment protocols: i) visual benthic reef surveys: ii) visual census of Autonomous Reef Monitoring Structures (ARMS) plates; and iii) metabarcoding techniques of the ARMS (including sessile, 106–500 μm and 500–2000 μm size fractions), that target the cryptic and exposed communities of three reefs in the central Red Sea. Visual census showed a dominance of Cnidaria (Anthozoa) and Rhodophyta on the reef substrate, while Porifera, Bryozoa and Rhodophyta were the most abundant groups on the ARMS plates. Metabarcoding, targeting the 18S rRNA gene, significantly increased estimates of the species diversity (p < 0.001); revealing that Annelida were generally the dominant phyla (in terms of reads) of all fractions and reefs. Furthermore, metabarcoding detected microbial eukaryotic groups such as Syndiniophyceae, Mamiellophyceae and Bacillariophyceae as relevant components of the sessile fraction. ANOSIM analysis showed that the three reef sites showed no differences based on the visual census data. Metabarcoding showed a higher sensitivity for identifying differences between reef communities at smaller geographic scales than standard visual census techniques as significant differences in the assemblages were observed amongst the reefs. Comparison of the techniques showed no similar patterns for the visual techniques while the metabarcoding of the ARMS showed similar patterns amongst fractions. Establishing ARMS as a standard tool in reef monitoring will not only advance our understanding of local processes and ecological community response to environmental changes, as different faunal components will provide complementary information but also improve the estimates of biodiversity in coral reef benthic communities. This study lays the foundations for further studies looking at integrating traditional reef survey methodologies with complementary approaches, such as metabarcoding, which investigate other components of the reef community.en
dc.description.sponsorshipThe authors would like to thank the skippers and crew from CMOR, KAUST who enabled the sampling to be performed. The authors are also indebted to the help given during sampling and laboratory work from Eva Aylagas Martinez and Miguel Viegas, as well as Saskia Kürten for undertaking the nutrient analysis. We would also like to thank Ute Langner for the production of the map. Furthermore, the authors would like to acknowledge the assistance provide in the improvement of this manuscript from the two reviewers. The research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST). This study was undertaken within the framework of the project DEVOTES (EU - ENV.2012.6.2–3 - Grant agreement 308392; http://www.devotes-project.eu).en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0141113616300617en
dc.rightsArchived with thanks to Marine Environmental Research, Under a Creative Commons license, http://creativecommons.org/licenses/by/4.0/en
dc.subjectAutonomous reef monitoring structuresen
dc.subjectBiodiversity surveysen
dc.subjectMetabarcodingen
dc.subjectCoral reefsen
dc.subjectRed Seaen
dc.subjectMarine ecologyen
dc.subjectBenthosen
dc.subjectMonitoringen
dc.titlePlease mind the gap – Visual census and cryptic biodiversity assessment at central Red Sea coral reefsen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMarine Environmental Researchen
dc.eprint.versionPost-printen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorPearman, John K.en
kaust.authorAnlauf, Holgeren
kaust.authorIrigoien, Xabieren
kaust.authorCarvalho, Susanaen
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