Hydroxylamine-dependent Anaerobic Ammonium Oxidation (Anammox) by “ Candidatus Brocadia sinica”

Handle URI:
http://hdl.handle.net/10754/608607
Title:
Hydroxylamine-dependent Anaerobic Ammonium Oxidation (Anammox) by “ Candidatus Brocadia sinica”
Authors:
Oshiki, Mamoru; Ali, Muhammad ( 0000-0003-3360-1622 ) ; Shinyako-Hata, Kaori; Satoh, Hisashi; Okabe, Satoshi
Abstract:
Although metabolic pathways and associated enzymes of anaerobic ammonium oxidation (anammox) of “Ca. Kuenenia stuttgartiensis” have been studied, those of other anammox bacteria are still poorly understood. NO2- reduction to NO is considered to be the first step in the anammox metabolism of “Ca. K. stuttgartiensis”, however, “Ca. Brocadia” lacks the genes that encode canonical NO-forming nitrite reductases (NirS or NirK) in its genome, which is different from “Ca. K. stuttgartiensis”. Here, we studied the anammox metabolism of “Ca. Brocadia sinica”. 15N-tracer experiments demonstrated that “Ca. B. sinica” cells could reduce NO2- to NH2OH, instead of NO, with as yet unidentified nitrite reductase(s). Furthermore, N2H4 synthesis, downstream reaction of NO2- reduction, was investigated using a purified “Ca. B. sinica” hydrazine synthase (Hzs) and intact cells. Both the “Ca. B. sinica” Hzs and cells utilized NH2OH and NH4+, but not NO and NH4+, for N2H4 synthesis and further oxidized N2H4 to N2 gas. Taken together, the metabolic pathway of “Ca. B. sinica” is NH2OH-dependent and different from the one of “Ca. K. stuttgartiensis”, indicating metabolic diversity of anammox bacteria. This article is protected by copyright. All rights reserved.
KAUST Department:
Water Desalination & Reuse Research Cntr; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Hydroxylamine-dependent Anaerobic Ammonium Oxidation (Anammox) by “ Candidatus Brocadia sinica” 2016 Environmental Microbiology
Publisher:
Wiley-Blackwell
Journal:
Environmental Microbiology
Issue Date:
26-Apr-2016
DOI:
10.1111/1462-2920.13355
Type:
Article
ISSN:
14622912
Sponsors:
This research was supported by the grants from the New Energy and Industrial Technology (NEDO), the Japan Science and Technology Agency (CREST), and The Institute for Fermentation Organization, which were granted to S.O. M.O. was supported by the grants from the Japan Society for the Promotion of Science, the Union Tool Co., the Steel Foundation for Environmental Protection Technology, and Yamaguchi Scholarship foundation. The authors acknowledge M. Waki and Y. Suwa for valuable advices for isotopomer analysis of N2 gas, M. Morikawa for valuable discussions and facilities for protein purification. Furthermore, M.O sincerely appreciates B. Kartal and J.T. Keltjens for valuable discussions, advices and suggestions to the present works.
Additional Links:
http://doi.wiley.com/10.1111/1462-2920.13355
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorOshiki, Mamoruen
dc.contributor.authorAli, Muhammaden
dc.contributor.authorShinyako-Hata, Kaorien
dc.contributor.authorSatoh, Hisashien
dc.contributor.authorOkabe, Satoshien
dc.date.accessioned2016-05-08T14:58:41Zen
dc.date.available2016-05-08T14:58:41Zen
dc.date.issued2016-04-26en
dc.identifier.citationHydroxylamine-dependent Anaerobic Ammonium Oxidation (Anammox) by “ Candidatus Brocadia sinica” 2016 Environmental Microbiologyen
dc.identifier.issn14622912en
dc.identifier.doi10.1111/1462-2920.13355en
dc.identifier.urihttp://hdl.handle.net/10754/608607en
dc.description.abstractAlthough metabolic pathways and associated enzymes of anaerobic ammonium oxidation (anammox) of “Ca. Kuenenia stuttgartiensis” have been studied, those of other anammox bacteria are still poorly understood. NO2- reduction to NO is considered to be the first step in the anammox metabolism of “Ca. K. stuttgartiensis”, however, “Ca. Brocadia” lacks the genes that encode canonical NO-forming nitrite reductases (NirS or NirK) in its genome, which is different from “Ca. K. stuttgartiensis”. Here, we studied the anammox metabolism of “Ca. Brocadia sinica”. 15N-tracer experiments demonstrated that “Ca. B. sinica” cells could reduce NO2- to NH2OH, instead of NO, with as yet unidentified nitrite reductase(s). Furthermore, N2H4 synthesis, downstream reaction of NO2- reduction, was investigated using a purified “Ca. B. sinica” hydrazine synthase (Hzs) and intact cells. Both the “Ca. B. sinica” Hzs and cells utilized NH2OH and NH4+, but not NO and NH4+, for N2H4 synthesis and further oxidized N2H4 to N2 gas. Taken together, the metabolic pathway of “Ca. B. sinica” is NH2OH-dependent and different from the one of “Ca. K. stuttgartiensis”, indicating metabolic diversity of anammox bacteria. This article is protected by copyright. All rights reserved.en
dc.description.sponsorshipThis research was supported by the grants from the New Energy and Industrial Technology (NEDO), the Japan Science and Technology Agency (CREST), and The Institute for Fermentation Organization, which were granted to S.O. M.O. was supported by the grants from the Japan Society for the Promotion of Science, the Union Tool Co., the Steel Foundation for Environmental Protection Technology, and Yamaguchi Scholarship foundation. The authors acknowledge M. Waki and Y. Suwa for valuable advices for isotopomer analysis of N2 gas, M. Morikawa for valuable discussions and facilities for protein purification. Furthermore, M.O sincerely appreciates B. Kartal and J.T. Keltjens for valuable discussions, advices and suggestions to the present works.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://doi.wiley.com/10.1111/1462-2920.13355en
dc.rightsThis is the peer reviewed version of the following article: Oshiki, M., Ali, M., Shinyako-Hata, K., Satoh, H. and Okabe, S. (2016), Hydroxylamine-dependent Anaerobic Ammonium Oxidation (Anammox) by “Candidatus Brocadia sinica”. Environmental Microbiology., which has been published in final form at http://doi.wiley.com/10.1111/1462-2920.13355. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.titleHydroxylamine-dependent Anaerobic Ammonium Oxidation (Anammox) by “ Candidatus Brocadia sinica”en
dc.typeArticleen
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalEnvironmental Microbiologyen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Civil Engineering; National Institute of Technology, Nagaoka College; Nagaoka Niigata 940-8532 Japanen
dc.contributor.institutionDivision of Environmental Engineering, Faculty of Engineering; Hokkaido University; North 13, West-8 Sapporo Hokkaido 060-8628 Japanen
dc.contributor.institutionTokyo Engineering Consultants Co., LTD.en
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAli, Muhammaden
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