Immune-Modulatory Genomic Properties Differentiate Gut Microbiotas of Infants with and without Eczema

Handle URI:
http://hdl.handle.net/10754/606956
Title:
Immune-Modulatory Genomic Properties Differentiate Gut Microbiotas of Infants with and without Eczema
Authors:
Yap, Gaik Chin; Liu, Wen Tso; Oh, Seungdae; Hong, Pei-Ying ( 0000-0002-4474-6600 ) ; Huang, Chiung Hui; Aw, Marion; Shek, Lynette; Wah, Bee
Abstract:
Background: The gastrointestinal tract is the primary site of interaction between the host immune system and microorganisms. Studies have suggested that selective microbial targets may influence the development of the allergic diseases. But the difference in functional gene composition remains unknown. We aim to assess the structural and functional gene composition of stool microbiota of infants with eczema and their matched (for age, gender, mode of delivery, feeding) controls at the age of 1 month. Methods: Twelve children with eczema and their controls were selected from the placebo arm of a birth cohort of at-risk infants participating in a randomized double-blind trial on the protective effects of supplemental probiotics in early life on allergic outcomes. The four were caesarean delivery followed by formula feeding (eczema = 2 and healthy control = 2) and the eight were vaginal delivery followed by partial breast feeding mixed with formula feeding (eczema = 4 and healthy control = 4). Bacterial genomic DNA were extracted from fecal samples and prepared for Illumina Miseq and Hiseq sequencing. Data analysis such as sequence quality check, contigs assembly and gene annotation were carried out for the DNA sequences obtained from Miseq and Hiseq sequencing. Results: Phylogenetic analysis of metagenomic sequences revealed that four phyla dominated both microbial communities: Proteobacteria (54% and 63% for healthy and eczema communities, respectively), Firmicutes (26% and 18%), Actinobacteria (13% and 8%), Bacteroidetes (7% and 8%). Comparative metagenomic analysis showed that immune-regulatory TCAAGCTTGA motifs were significantly enriched in healthy communities, many of which were encoded by Bifidobacterium (38% of the total motifs in the healthy communities). Draft genomes of five Bifidobacterium species (B. longum, B. bifidum, B. breve, B. dentium, and B. pseudocatenulatum ) were recovered from metagenomic datasets. The B. longum BFN-121- 2 genome encoded more TCAAGCTTGA motifs (4.2 copies per 1 million genome sequence) than other Bifidobacterium genomes and was significantly overrepresented (P < 0.05) in the healthy communities. Conclusions: Our results report distinct immune-modulatory genomic properties of gut microbiotas in healthy infants as compared to children with eczema and provide new insights into potential roles of gut microbiotas in affecting human immune homeostasis.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Springer Science + Business Media
Journal:
World Allergy Organization Journal
Conference/Event name:
XXIV World Allergy Congress 2015
Issue Date:
14-Oct-2015
DOI:
10.1186/s40413-016-0096-1
Type:
Presentation
Additional Links:
http://waojournal.biomedcentral.com/articles/10.1186/s40413-016-0096-1
Appears in Collections:
Presentations; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorYap, Gaik Chinen
dc.contributor.authorLiu, Wen Tsoen
dc.contributor.authorOh, Seungdaeen
dc.contributor.authorHong, Pei-Yingen
dc.contributor.authorHuang, Chiung Huien
dc.contributor.authorAw, Marionen
dc.contributor.authorShek, Lynetteen
dc.contributor.authorWah, Beeen
dc.date.accessioned2016-04-25T13:39:15Zen
dc.date.available2016-04-25T13:39:15Zen
dc.date.issued2015-10-14en
dc.identifier.doi10.1186/s40413-016-0096-1en
dc.identifier.urihttp://hdl.handle.net/10754/606956en
dc.description.abstractBackground: The gastrointestinal tract is the primary site of interaction between the host immune system and microorganisms. Studies have suggested that selective microbial targets may influence the development of the allergic diseases. But the difference in functional gene composition remains unknown. We aim to assess the structural and functional gene composition of stool microbiota of infants with eczema and their matched (for age, gender, mode of delivery, feeding) controls at the age of 1 month. Methods: Twelve children with eczema and their controls were selected from the placebo arm of a birth cohort of at-risk infants participating in a randomized double-blind trial on the protective effects of supplemental probiotics in early life on allergic outcomes. The four were caesarean delivery followed by formula feeding (eczema = 2 and healthy control = 2) and the eight were vaginal delivery followed by partial breast feeding mixed with formula feeding (eczema = 4 and healthy control = 4). Bacterial genomic DNA were extracted from fecal samples and prepared for Illumina Miseq and Hiseq sequencing. Data analysis such as sequence quality check, contigs assembly and gene annotation were carried out for the DNA sequences obtained from Miseq and Hiseq sequencing. Results: Phylogenetic analysis of metagenomic sequences revealed that four phyla dominated both microbial communities: Proteobacteria (54% and 63% for healthy and eczema communities, respectively), Firmicutes (26% and 18%), Actinobacteria (13% and 8%), Bacteroidetes (7% and 8%). Comparative metagenomic analysis showed that immune-regulatory TCAAGCTTGA motifs were significantly enriched in healthy communities, many of which were encoded by Bifidobacterium (38% of the total motifs in the healthy communities). Draft genomes of five Bifidobacterium species (B. longum, B. bifidum, B. breve, B. dentium, and B. pseudocatenulatum ) were recovered from metagenomic datasets. The B. longum BFN-121- 2 genome encoded more TCAAGCTTGA motifs (4.2 copies per 1 million genome sequence) than other Bifidobacterium genomes and was significantly overrepresented (P < 0.05) in the healthy communities. Conclusions: Our results report distinct immune-modulatory genomic properties of gut microbiotas in healthy infants as compared to children with eczema and provide new insights into potential roles of gut microbiotas in affecting human immune homeostasis.en
dc.publisherSpringer Science + Business Mediaen
dc.relation.urlhttp://waojournal.biomedcentral.com/articles/10.1186/s40413-016-0096-1en
dc.titleImmune-Modulatory Genomic Properties Differentiate Gut Microbiotas of Infants with and without Eczemaen
dc.typePresentationen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalWorld Allergy Organization Journalen
dc.conference.date14-17 October 2015en
dc.conference.nameXXIV World Allergy Congress 2015en
dc.conference.locationSeoul, Koreaen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionUniversity of Illinois at Urbana-Champaign, USAen
dc.contributor.institutionNational University Health System, Singaporeen
dc.contributor.institutionNational University of Singapore, Singaporeen
kaust.authorHong, Pei Yingen
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