Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

Handle URI:
http://hdl.handle.net/10754/604744
Title:
Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea
Authors:
Ettoumi, Besma; Chouchane, Habib; Guesmi, Amel; Mahjoubi, Mouna; Brusetti, Lorenzo; Neifar, Mohamed; Borin, Sara; Daffonchio, Daniele ( 0000-0003-0947-925X ) ; Cherif, Ameur
Abstract:
In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n = 49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n = 22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea 2016 Microbiological Research
Publisher:
Elsevier BV
Journal:
Microbiological Research
Issue Date:
1-Apr-2016
DOI:
10.1016/j.micres.2016.03.006
Type:
Article
ISSN:
09445013
Sponsors:
This work was performed in the frame of the CIESM-SUB programme (CIESM Sur-Un- Bateau Cruise I). Pr. Laura Giuliano, who made possible this multidisciplinary research, Pr. Giorgio Budillon, the Chief Scientist of the expedition, the captains Angelo Barca and crews of the R/V Universitatis, are gratefully acknowledged. We thank Dr. Mirko Magagnini for his help in samples collection. This work was partly supported by the Ministry of High Education and Scientific Research of the Tunisian Republic, Grant LR MBA206 and LR11ES31, and the European Union in the ambit of project ULIXES (FP7-KBBE-2010-4, CP-FP-SICA under grant agreement no. 266473).
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0944501316300817
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorEttoumi, Besmaen
dc.contributor.authorChouchane, Habiben
dc.contributor.authorGuesmi, Amelen
dc.contributor.authorMahjoubi, Mounaen
dc.contributor.authorBrusetti, Lorenzoen
dc.contributor.authorNeifar, Mohameden
dc.contributor.authorBorin, Saraen
dc.contributor.authorDaffonchio, Danieleen
dc.contributor.authorCherif, Ameuren
dc.date.accessioned2016-04-07T13:55:36Zen
dc.date.available2016-04-07T13:55:36Zen
dc.date.issued2016-04-01en
dc.identifier.citationDiversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea 2016 Microbiological Researchen
dc.identifier.issn09445013en
dc.identifier.doi10.1016/j.micres.2016.03.006en
dc.identifier.urihttp://hdl.handle.net/10754/604744en
dc.description.abstractIn the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n = 49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n = 22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.en
dc.description.sponsorshipThis work was performed in the frame of the CIESM-SUB programme (CIESM Sur-Un- Bateau Cruise I). Pr. Laura Giuliano, who made possible this multidisciplinary research, Pr. Giorgio Budillon, the Chief Scientist of the expedition, the captains Angelo Barca and crews of the R/V Universitatis, are gratefully acknowledged. We thank Dr. Mirko Magagnini for his help in samples collection. This work was partly supported by the Ministry of High Education and Scientific Research of the Tunisian Republic, Grant LR MBA206 and LR11ES31, and the European Union in the ambit of project ULIXES (FP7-KBBE-2010-4, CP-FP-SICA under grant agreement no. 266473).en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0944501316300817en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microbiological Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microbiological Research, 1 April 2016. DOI: 10.1016/j.micres.2016.03.006en
dc.subjectDeep sea sedimentsen
dc.subjectActinobacteriaen
dc.subjectARISA fingerprintingen
dc.subjectBOX-PCRen
dc.subjectbiosurfactanten
dc.titleDiversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Seaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMicrobiological Researchen
dc.eprint.versionPost-printen
dc.contributor.institutionLaboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, 2092 Tunis, Tunisiaen
dc.contributor.institutionDepartment of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, 20133 Milan, Italyen
dc.contributor.institutionUniv. Manouba, ISBST, BVBGR-LR11ES31, Biotechpole of Sidi Thabet, 2020 Ariana, Tunisiaen
dc.contributor.institutionFaculty of Science and Technology, Free University of Bozen/Bolzano, Bolzano, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDaffonchio, Danieleen
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