Marine-derived collagen biomaterials from echinoderm connective tissues

Handle URI:
http://hdl.handle.net/10754/604714
Title:
Marine-derived collagen biomaterials from echinoderm connective tissues
Authors:
Ferrario, Cinzia ( 0000-0002-1804-4760 ) ; Leggio, Livio ( 0000-0002-4599-4972 ) ; Leone, Roberta ( 0000-0003-2034-943X ) ; Di Benedetto, Cristiano ( 0000-0002-8662-7044 ) ; Guidetti, Luca ( 0000-0003-2671-811X ) ; Coccè, Valentina ( 0000-0002-9451-9529 ) ; Ascagni, Miriam ( 0000-0001-5458-269X ) ; Bonasoro, Francesco ( 0000-0002-1559-0707 ) ; La Porta, Caterina A.M. ( 0000-0002-3010-8966 ) ; Candia Carnevali, M. Daniela ( 0000-0002-0215-8843 ) ; Sugni, Michela ( 0000-0002-4574-5802 )
Abstract:
The use of marine collagens is a hot topic in the field of tissue engineering. Echinoderms possess unique connective tissues (Mutable Collagenous Tissues, MCTs) which can represent an innovative source of collagen to develop collagen barrier-membranes for Guided Tissue Regeneration (GTR). In the present work we used MCTs from different echinoderm models (sea urchin, starfish and sea cucumber) to produce echinoderm-derived collagen membranes (EDCMs). Commercial membranes for GTR or soluble/reassembled (fibrillar) bovine collagen substrates were used as controls. The three EDCMs were similar among each other in terms of structure and mechanical performances and were much thinner and mechanically more resistant than the commercial membranes. Number of fibroblasts seeded on sea-urchin membranes were comparable to the bovine collagen substrates. Cell morphology on all EDCMs was similar to that of structurally comparable (reassembled) bovine collagen substrates. Overall, echinoderms, and sea urchins particularly, are alternative collagen sources to produce efficient GTR membranes. Sea urchins display a further advantage in terms of eco-sustainability by recycling tissues from food wastes.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Marine-derived collagen biomaterials from echinoderm connective tissues 2016 Marine Environmental Research
Publisher:
Elsevier BV
Journal:
Marine Environmental Research
Issue Date:
31-Mar-2016
DOI:
10.1016/j.marenvres.2016.03.007
Type:
Article
ISSN:
01411136
Sponsors:
This research was funded by Young Researcher Grant (University of Milan, PI: Dr. Michela Sugni). We are grateful to the Marine Protected Area of Portofino (GE, Italy) for permission to collect experimental animals and to the scuba diver Dr. Dario Fassini for helping during the collection. We would like to deeply thank Dr. Paolo Tremolada for his support with statistical analyses, Andrea Spalletti and Silvia Colombo for helping in cell culture experiments, Prof. Iain Wilkie for mechanical equipment use and Dr. Dario Fassini for welcome comments on mechanical tests. We also thank Dr. Elena Canciani for providing commercial membranes used in this work.
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0141113616300320
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorFerrario, Cinziaen
dc.contributor.authorLeggio, Livioen
dc.contributor.authorLeone, Robertaen
dc.contributor.authorDi Benedetto, Cristianoen
dc.contributor.authorGuidetti, Lucaen
dc.contributor.authorCoccè, Valentinaen
dc.contributor.authorAscagni, Miriamen
dc.contributor.authorBonasoro, Francescoen
dc.contributor.authorLa Porta, Caterina A.M.en
dc.contributor.authorCandia Carnevali, M. Danielaen
dc.contributor.authorSugni, Michelaen
dc.date.accessioned2016-04-07T09:09:34Zen
dc.date.available2016-04-07T09:09:34Zen
dc.date.issued2016-03-31en
dc.identifier.citationMarine-derived collagen biomaterials from echinoderm connective tissues 2016 Marine Environmental Researchen
dc.identifier.issn01411136en
dc.identifier.doi10.1016/j.marenvres.2016.03.007en
dc.identifier.urihttp://hdl.handle.net/10754/604714en
dc.description.abstractThe use of marine collagens is a hot topic in the field of tissue engineering. Echinoderms possess unique connective tissues (Mutable Collagenous Tissues, MCTs) which can represent an innovative source of collagen to develop collagen barrier-membranes for Guided Tissue Regeneration (GTR). In the present work we used MCTs from different echinoderm models (sea urchin, starfish and sea cucumber) to produce echinoderm-derived collagen membranes (EDCMs). Commercial membranes for GTR or soluble/reassembled (fibrillar) bovine collagen substrates were used as controls. The three EDCMs were similar among each other in terms of structure and mechanical performances and were much thinner and mechanically more resistant than the commercial membranes. Number of fibroblasts seeded on sea-urchin membranes were comparable to the bovine collagen substrates. Cell morphology on all EDCMs was similar to that of structurally comparable (reassembled) bovine collagen substrates. Overall, echinoderms, and sea urchins particularly, are alternative collagen sources to produce efficient GTR membranes. Sea urchins display a further advantage in terms of eco-sustainability by recycling tissues from food wastes.en
dc.description.sponsorshipThis research was funded by Young Researcher Grant (University of Milan, PI: Dr. Michela Sugni). We are grateful to the Marine Protected Area of Portofino (GE, Italy) for permission to collect experimental animals and to the scuba diver Dr. Dario Fassini for helping during the collection. We would like to deeply thank Dr. Paolo Tremolada for his support with statistical analyses, Andrea Spalletti and Silvia Colombo for helping in cell culture experiments, Prof. Iain Wilkie for mechanical equipment use and Dr. Dario Fassini for welcome comments on mechanical tests. We also thank Dr. Elena Canciani for providing commercial membranes used in this work.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0141113616300320en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Marine Environmental Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Marine Environmental Research, 31 March 2016. DOI: 10.1016/j.marenvres.2016.03.007en
dc.subject“Blue” biotechnologyen
dc.subjectalternative biomaterialen
dc.subjectmarine collagenen
dc.subjectechinodermsen
dc.subjectMutable Collagenous Tissuesen
dc.subjecthuman fibroblast cultureen
dc.subjectGuided Tissue Regenerationen
dc.subjectenvironmental impacten
dc.subjectby-product valorisationen
dc.titleMarine-derived collagen biomaterials from echinoderm connective tissuesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMarine Environmental Researchen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Biosciences, University of Milan, via Celoria, 26, 20133, Milan, Italyen
dc.contributor.institutionFondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Maxillofacial and Dental Unit, Department of Biomedical, Surgical and Dental Sciences, University of Milan, via Commenda, 10, 20122, Milan, Italyen
dc.contributor.institutionCenter for Complexity and Biosystems, University of Milan, via Celoria, 16, 20133, Milan, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDi Benedetto, Cristianoen
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