Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with roles in Plant Cell Wall Degradation and Virulence

Handle URI:
http://hdl.handle.net/10754/604367
Title:
Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with roles in Plant Cell Wall Degradation and Virulence
Authors:
Lakshman, Dilip; Roberts, Daniel P.; Garrett, Wesley M.; Natarajan, Savithiry S.; Darwish, Omar; Alkharouf, Nadim; Pain, Arnab ( 0000-0002-1755-2819 ) ; Khan, Farooq; Jambhulkar, Prashant P.; Mitra, Amitava
Abstract:
Rhizoctonia solani AG 4 is a soilborne necrotrophic fungal plant pathogen that causes economically important diseases on agronomic crops worldwide. Here we used a proteomics approach to characterize both intracellular proteins and the secretome of R. solani AG 4 isolate Rs23A under several growth conditions; the secretome being highly important in pathogenesis. From over 500 total secretome and soluble intracellular protein spots from 2-D gels, 457 protein spots were analyzed and 318 proteins positively matched with fungal proteins of known function by comparison with available R. solani genome databases specific for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized to possible cellular locations and functional groups; and for some proteins their putative roles in plant cell wall degradation and virulence. The majority of the secreted proteins were grouped to extracellular regions and contain hydrolase activity.
KAUST Department:
Pathogen Genomics Laboratory
Citation:
Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with roles in Plant Cell Wall Degradation and Virulence. 2016 Journal of Agricultural and Food Chemistry
Publisher:
American Chemical Society (ACS)
Journal:
Journal of Agricultural and Food Chemistry
Issue Date:
28-Mar-2016
DOI:
10.1021/acs.jafc.5b05735
Type:
Article
ISSN:
0021-8561; 1520-5118
Sponsors:
We would like to thank S. Lakshman for a portion of the technical support.
Additional Links:
http://pubs.acs.org/doi/abs/10.1021/acs.jafc.5b05735
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorLakshman, Dilipen
dc.contributor.authorRoberts, Daniel P.en
dc.contributor.authorGarrett, Wesley M.en
dc.contributor.authorNatarajan, Savithiry S.en
dc.contributor.authorDarwish, Omaren
dc.contributor.authorAlkharouf, Nadimen
dc.contributor.authorPain, Arnaben
dc.contributor.authorKhan, Farooqen
dc.contributor.authorJambhulkar, Prashant P.en
dc.contributor.authorMitra, Amitavaen
dc.date.accessioned2016-04-04T13:19:04Zen
dc.date.available2016-04-04T13:19:04Zen
dc.date.issued2016-03-28en
dc.identifier.citationProteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with roles in Plant Cell Wall Degradation and Virulence. 2016 Journal of Agricultural and Food Chemistryen
dc.identifier.issn0021-8561en
dc.identifier.issn1520-5118en
dc.identifier.doi10.1021/acs.jafc.5b05735en
dc.identifier.urihttp://hdl.handle.net/10754/604367en
dc.description.abstractRhizoctonia solani AG 4 is a soilborne necrotrophic fungal plant pathogen that causes economically important diseases on agronomic crops worldwide. Here we used a proteomics approach to characterize both intracellular proteins and the secretome of R. solani AG 4 isolate Rs23A under several growth conditions; the secretome being highly important in pathogenesis. From over 500 total secretome and soluble intracellular protein spots from 2-D gels, 457 protein spots were analyzed and 318 proteins positively matched with fungal proteins of known function by comparison with available R. solani genome databases specific for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized to possible cellular locations and functional groups; and for some proteins their putative roles in plant cell wall degradation and virulence. The majority of the secreted proteins were grouped to extracellular regions and contain hydrolase activity.en
dc.description.sponsorshipWe would like to thank S. Lakshman for a portion of the technical support.en
dc.language.isoenen
dc.publisherAmerican Chemical Society (ACS)en
dc.relation.urlhttp://pubs.acs.org/doi/abs/10.1021/acs.jafc.5b05735en
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.jafc.5b05735.en
dc.titleProteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with roles in Plant Cell Wall Degradation and Virulenceen
dc.typeArticleen
dc.contributor.departmentPathogen Genomics Laboratoryen
dc.identifier.journalJournal of Agricultural and Food Chemistryen
dc.eprint.versionPost-printen
dc.contributor.institutionUSDA-ARS, Beltsville, Maryland 20705, USAen
dc.contributor.institutionComputer and Information Sciences, Towson University, Towson, Maryland 21252en
dc.contributor.institutionDept. of Plant Pathology, MPUA&T, Udaipur PIN 327001, Indiaen
dc.contributor.institutionDept. of Plant Pathology, University of Nebraska, Nebraska, 68583, USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorPain, Arnaben
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