Microbial oil-degradation under mild hydrostatic pressure (10 MPa): which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria?

Handle URI:
http://hdl.handle.net/10754/603958
Title:
Microbial oil-degradation under mild hydrostatic pressure (10 MPa): which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria?
Authors:
Scoma, Alberto; Barbato, Marta; Hernandez-Sanabria, Emma; Mapelli, Francesca; Daffonchio, Daniele ( 0000-0003-0947-925X ) ; Borin, Sara; Boon, Nico
Abstract:
Oil spills represent an overwhelming carbon input to the marine environment that immediately impacts the sea surface ecosystem. Microbial communities degrading the oil fraction that eventually sinks to the seafloor must also deal with hydrostatic pressure, which linearly increases with depth. Piezosensitive hydrocarbonoclastic bacteria are ideal candidates to elucidate impaired pathways following oil spills at low depth. In the present paper, we tested two strains of the ubiquitous Alcanivorax genus, namely A. jadensis KS_339 and A. dieselolei KS_293, which is known to rapidly grow after oil spills. Strains were subjected to atmospheric and mild pressure (0.1, 5 and 10 MPa, corresponding to a depth of 0, 500 and 1000 m, respectively) providing n-dodecane as sole carbon source. Pressures equal to 5 and 10 MPa significantly lowered growth yields of both strains. However, in strain KS_293 grown at 10 MPa CO2 production per cell was not affected, cell integrity was preserved and PO43− uptake increased. Analysis of its transcriptome revealed that 95% of its genes were downregulated. Increased transcription involved protein synthesis, energy generation and respiration pathways. Interplay between these factors may play a key role in shaping the structure of microbial communities developed after oil spills at low depth and limit their bioremediation potential.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Microbial oil-degradation under mild hydrostatic pressure (10 MPa): which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria? 2016, 6:23526 Scientific Reports
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
29-Mar-2016
DOI:
10.1038/srep23526
Type:
Article
ISSN:
2045-2322
Sponsors:
This work was funded by FP-7 project Kill Spill (No. 312139, “Integrated Biotechnological Solutions for Combating Marine Oil Spills”). The authors thank the support of King Abdullah University of Science and Technology (baseline research funds to D.D.). F.M. was supported by Università degli Studi di Milano, DeFENS, European Social Found (FSE) and Regione Lombardia (contract “Dote Ricerca”). Mr. Benjamin Buysschaert and Ms. Nicole Hahn are kindly acknowledged for their help with flow cytometry analyses.
Additional Links:
http://www.nature.com/articles/srep23526
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorScoma, Albertoen
dc.contributor.authorBarbato, Martaen
dc.contributor.authorHernandez-Sanabria, Emmaen
dc.contributor.authorMapelli, Francescaen
dc.contributor.authorDaffonchio, Danieleen
dc.contributor.authorBorin, Saraen
dc.contributor.authorBoon, Nicoen
dc.date.accessioned2016-03-30T07:41:58Zen
dc.date.available2016-03-30T07:41:58Zen
dc.date.issued2016-03-29en
dc.identifier.citationMicrobial oil-degradation under mild hydrostatic pressure (10 MPa): which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria? 2016, 6:23526 Scientific Reportsen
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep23526en
dc.identifier.urihttp://hdl.handle.net/10754/603958en
dc.description.abstractOil spills represent an overwhelming carbon input to the marine environment that immediately impacts the sea surface ecosystem. Microbial communities degrading the oil fraction that eventually sinks to the seafloor must also deal with hydrostatic pressure, which linearly increases with depth. Piezosensitive hydrocarbonoclastic bacteria are ideal candidates to elucidate impaired pathways following oil spills at low depth. In the present paper, we tested two strains of the ubiquitous Alcanivorax genus, namely A. jadensis KS_339 and A. dieselolei KS_293, which is known to rapidly grow after oil spills. Strains were subjected to atmospheric and mild pressure (0.1, 5 and 10 MPa, corresponding to a depth of 0, 500 and 1000 m, respectively) providing n-dodecane as sole carbon source. Pressures equal to 5 and 10 MPa significantly lowered growth yields of both strains. However, in strain KS_293 grown at 10 MPa CO2 production per cell was not affected, cell integrity was preserved and PO43− uptake increased. Analysis of its transcriptome revealed that 95% of its genes were downregulated. Increased transcription involved protein synthesis, energy generation and respiration pathways. Interplay between these factors may play a key role in shaping the structure of microbial communities developed after oil spills at low depth and limit their bioremediation potential.en
dc.description.sponsorshipThis work was funded by FP-7 project Kill Spill (No. 312139, “Integrated Biotechnological Solutions for Combating Marine Oil Spills”). The authors thank the support of King Abdullah University of Science and Technology (baseline research funds to D.D.). F.M. was supported by Università degli Studi di Milano, DeFENS, European Social Found (FSE) and Regione Lombardia (contract “Dote Ricerca”). Mr. Benjamin Buysschaert and Ms. Nicole Hahn are kindly acknowledged for their help with flow cytometry analyses.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/articles/srep23526en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleMicrobial oil-degradation under mild hydrostatic pressure (10 MPa): which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria?en
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionLaboratory of Microbial Ecology and Technology (LabMET), University of Gent, Coupure Links 653, B 9000 Gent, Belgiumen
dc.contributor.institutionDepartment of Food, Environmental and Nutritional Sciences (DeFENS), University of Milano, Via Celoria 2, 20133, Milano, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDaffonchio, Danieleen
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