Inclusion of gold nanoparticles in meso-porous silicon for the SERS analysis of cell adhesion on nano-structured surfaces

Handle URI:
http://hdl.handle.net/10754/603715
Title:
Inclusion of gold nanoparticles in meso-porous silicon for the SERS analysis of cell adhesion on nano-structured surfaces
Authors:
Coluccio, M.L. ( 0000-0002-9987-2348 ) ; De Vitis, S.; Strumbo, G.; Candeloro, P.; Perozziello, G.; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 ) ; Gentile, F.
Abstract:
The study and the comprehension of the mechanism of cell adhesion and cell interaction with a substrate is a key point when biology and medicine meet engineering. This is the case of several biomedical applications, from regenerative medicine and tissue engineering to lab on chip and many others, in which the realization of the appropriate artificial surface allows the control of cell adhesion and proliferation. In this context, we aimed to design and develop a fabrication method of mesoporous (MeP) silicon substrates, doped with gold nanoparticles, in which we combine the capability of porous surfaces to support cell adhesion with the SERS capabilities of gold nanoparticles, to understand the chemical mechanisms of cell/surface interaction. MeP Si surfaces were realized by anodization of a Si wafer, creating the device for cell adhesion and growth. Gold nanoparticles were deposited on porous silicon by an electroless technique. We thus obtained devices with superior SERS capabilities, whereby cell activity may be controlled using Raman spectroscopy. MCF-7 breast cancer cells were cultured on the described substrates and SERS maps revealing the different expression and distribution of adhesion molecules were obtained by Raman spectroscopic analyses.
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Citation:
Inclusion of gold nanoparticles in meso-porous silicon for the SERS analysis of cell adhesion on nano-structured surfaces 2016 Microelectronic Engineering
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
25-Mar-2016
DOI:
10.1016/j.mee.2016.03.045
Type:
Article
ISSN:
01679317
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0167931716301575
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorColuccio, M.L.en
dc.contributor.authorDe Vitis, S.en
dc.contributor.authorStrumbo, G.en
dc.contributor.authorCandeloro, P.en
dc.contributor.authorPerozziello, G.en
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.contributor.authorGentile, F.en
dc.date.accessioned2016-03-27T12:30:22Zen
dc.date.available2016-03-27T12:30:22Zen
dc.date.issued2016-03-25en
dc.identifier.citationInclusion of gold nanoparticles in meso-porous silicon for the SERS analysis of cell adhesion on nano-structured surfaces 2016 Microelectronic Engineeringen
dc.identifier.issn01679317en
dc.identifier.doi10.1016/j.mee.2016.03.045en
dc.identifier.urihttp://hdl.handle.net/10754/603715en
dc.description.abstractThe study and the comprehension of the mechanism of cell adhesion and cell interaction with a substrate is a key point when biology and medicine meet engineering. This is the case of several biomedical applications, from regenerative medicine and tissue engineering to lab on chip and many others, in which the realization of the appropriate artificial surface allows the control of cell adhesion and proliferation. In this context, we aimed to design and develop a fabrication method of mesoporous (MeP) silicon substrates, doped with gold nanoparticles, in which we combine the capability of porous surfaces to support cell adhesion with the SERS capabilities of gold nanoparticles, to understand the chemical mechanisms of cell/surface interaction. MeP Si surfaces were realized by anodization of a Si wafer, creating the device for cell adhesion and growth. Gold nanoparticles were deposited on porous silicon by an electroless technique. We thus obtained devices with superior SERS capabilities, whereby cell activity may be controlled using Raman spectroscopy. MCF-7 breast cancer cells were cultured on the described substrates and SERS maps revealing the different expression and distribution of adhesion molecules were obtained by Raman spectroscopic analyses.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0167931716301575en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, 25 March 2016. DOI: 10.1016/j.mee.2016.03.045en
dc.subjectNano-topographyen
dc.subjectCell surface interactionen
dc.subjectElectroless depositionen
dc.subjectGold nanoparticlesen
dc.subjectSERSen
dc.titleInclusion of gold nanoparticles in meso-porous silicon for the SERS analysis of cell adhesion on nano-structured surfacesen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalMicroelectronic Engineeringen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University Magna Graecia, Catanzaro, Italyen
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University Federico II, Naples, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDi Fabrizio, Enzo M.en
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