Protein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imaging

Handle URI:
http://hdl.handle.net/10754/603622
Title:
Protein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imaging
Authors:
Croissant, Jonas G.; Zhang, Dingyuan; Alsaiari, Shahad K.; Lu, Jie; Deng, Lin ( 0000-0001-8954-5610 ) ; Tamanoi, Fuyuhiko; Zink, Jeffrey I.; Khashab, Niveen M. ( 0000-0003-2728-0666 )
Abstract:
Functional nanocarriers capable of transporting high drug contents without premature leakage and to controllably deliver several drugs are needed for better cancer treatments. To address this clinical need, gold cluster bovine serum albumin (AuNC@BSA) nanogates were engineered on mesoporous silica nanoparticles (MSN) for high drug loadings and co-delivery of two different anticancer drugs. The first drug, gemcitabine (GEM, 40 wt%), was loaded in positively-charged ammonium-functionalized MSN (MSN-NH3+). The second drug, doxorubicin (DOX, 32 wt%), was bound with negatively-charged AuNC@BSA electrostatically-attached onto MSN-NH3+, affording highly loaded pH-responsive MSN-AuNC@BSA nanocarriers. The co-delivery of DOX and GEM was achieved for the first time via an inorganic nanocarrier, possessing a zero-premature leakage behavior as well as drug loading capacities seven times higher than polymersome NPs. Besides, unlike the majority of strategies used to cap the pores of MSN, AuNC@BSA nanogates are biotools and were applied for targeted red nuclear staining and in-vivo tumor imaging. The straightforward non-covalent combination of MSN and gold-protein cluster bioconjugates thus leads to a simple, yet multifunctional nanotheranostic for the next generation of cancer treatments.
KAUST Department:
Smart Hybrid Materials Laboratory; Advanced Membranes and Porous Materials Center (AMPMC)
Citation:
Protein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imaging 2016 Journal of Controlled Release
Publisher:
Elsevier BV
Journal:
Journal of Controlled Release
Issue Date:
23-Mar-2016
DOI:
10.1016/j.jconrel.2016.03.030
Type:
Article
ISSN:
01683659
Sponsors:
The authors gratefully acknowledge King Abdullah University of Science and Technology (KAUST) and the University of California, Los Angeles for the support of this work. NIH grant CA133697. Authors thank Dr. Chong H. Chang for the ICP-AES measurements.
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S016836591630164X
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Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorCroissant, Jonas G.en
dc.contributor.authorZhang, Dingyuanen
dc.contributor.authorAlsaiari, Shahad K.en
dc.contributor.authorLu, Jieen
dc.contributor.authorDeng, Linen
dc.contributor.authorTamanoi, Fuyuhikoen
dc.contributor.authorZink, Jeffrey I.en
dc.contributor.authorKhashab, Niveen M.en
dc.date.accessioned2016-03-24T13:14:27Zen
dc.date.available2016-03-24T13:14:27Zen
dc.date.issued2016-03-23en
dc.identifier.citationProtein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imaging 2016 Journal of Controlled Releaseen
dc.identifier.issn01683659en
dc.identifier.doi10.1016/j.jconrel.2016.03.030en
dc.identifier.urihttp://hdl.handle.net/10754/603622en
dc.description.abstractFunctional nanocarriers capable of transporting high drug contents without premature leakage and to controllably deliver several drugs are needed for better cancer treatments. To address this clinical need, gold cluster bovine serum albumin (AuNC@BSA) nanogates were engineered on mesoporous silica nanoparticles (MSN) for high drug loadings and co-delivery of two different anticancer drugs. The first drug, gemcitabine (GEM, 40 wt%), was loaded in positively-charged ammonium-functionalized MSN (MSN-NH3+). The second drug, doxorubicin (DOX, 32 wt%), was bound with negatively-charged AuNC@BSA electrostatically-attached onto MSN-NH3+, affording highly loaded pH-responsive MSN-AuNC@BSA nanocarriers. The co-delivery of DOX and GEM was achieved for the first time via an inorganic nanocarrier, possessing a zero-premature leakage behavior as well as drug loading capacities seven times higher than polymersome NPs. Besides, unlike the majority of strategies used to cap the pores of MSN, AuNC@BSA nanogates are biotools and were applied for targeted red nuclear staining and in-vivo tumor imaging. The straightforward non-covalent combination of MSN and gold-protein cluster bioconjugates thus leads to a simple, yet multifunctional nanotheranostic for the next generation of cancer treatments.en
dc.description.sponsorshipThe authors gratefully acknowledge King Abdullah University of Science and Technology (KAUST) and the University of California, Los Angeles for the support of this work. NIH grant CA133697. Authors thank Dr. Chong H. Chang for the ICP-AES measurements.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S016836591630164Xen
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Journal of Controlled Release. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Controlled Release, 23 March 2016. DOI: 10.1016/j.jconrel.2016.03.030en
dc.subjectMesoporous silica nanoparticlesen
dc.subjectGold nanoclustersen
dc.subjectBovine serum albuminen
dc.subjectMulti-drug deliveryen
dc.subjectCanceren
dc.titleProtein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imagingen
dc.typeArticleen
dc.contributor.departmentSmart Hybrid Materials Laboratoryen
dc.contributor.departmentAdvanced Membranes and Porous Materials Center (AMPMC)en
dc.identifier.journalJournal of Controlled Releaseen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Chemistry and Biochemistry, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, California, USAen
dc.contributor.institutionDepartment of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, California, USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorCroissant, Jonas G.en
kaust.authorZhang, Dingyuanen
kaust.authorAlsaiari, Shahad K.en
kaust.authorDeng, Linen
kaust.authorKhashab, Niveen M.en
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