Nitrogen-fixing bacteria in Mediterranean seagrass (Posidonia oceanica) roots

Handle URI:
http://hdl.handle.net/10754/601136
Title:
Nitrogen-fixing bacteria in Mediterranean seagrass (Posidonia oceanica) roots
Authors:
Garcias Bonet, Neus ( 0000-0001-6469-7167 ) ; Arrieta, Jesus ( 0000-0002-0190-6950 ) ; Duarte, Carlos M. ( 0000-0002-1213-1361 ) ; Marbà, Núria
Abstract:
Biological nitrogen fixation by diazotrophic bacteria in seagrass rhizosphere and leaf epiphytic community is an important source of nitrogen required for plant growth. However, the presence of endophytic diazotrophs remains unclear in seagrass tissues. Here, we assess the presence, diversity and taxonomy of nitrogen-fixing bacteria within surface-sterilized roots of Posidonia oceanica. Moreover, we analyze the nitrogen isotopic signature of seagrass tissues in order to notice atmospheric nitrogen fixation. We detected nitrogen-fixing bacteria by nifH gene amplification in 13 out of the 78 roots sampled, corresponding to 9 locations out of 26 meadows. We detected two different types of bacterial nifH sequences associated with P. oceanica roots, which were closely related to sequences previously isolated from the rhizosphere of a salt marsh cord grass and a putative anaerobe. Nitrogen content of seagrass tissues showed low isotopic signatures in all the sampled meadows, pointing out the atmospheric origin of the assimilated nitrogen by seagrasses. However, this was not related with the presence of endophytic nitrogen fixers, suggesting the nitrogen fixation occurring in rhizosphere and in the epiphytic community could be an important source of nitrogen for P. oceanica. The low diversity of nitrogen-fixing bacteria reported here suggests species-specific relationships between diazotrophs and P. oceanica, revealing possible symbiotic interactions that could play a major role in nitrogen acquisition by seagrasses in oligotrophic environments where they form lush meadows.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Nitrogen-fixing bacteria in Mediterranean seagrass (Posidonia oceanica) roots 2016 Aquatic Botany
Publisher:
Elsevier BV
Journal:
Aquatic Botany
Issue Date:
9-Mar-2016
DOI:
10.1016/j.aquabot.2016.03.002
Type:
Article
ISSN:
03043770
Sponsors:
This study was funded by the projects MEDEICG and ESTRESX of the Spanish Marine Science and Technology Program (CTM2009-07013, CTM2012-32603). N.G.B. was supported by a PhD grant from the Government of Balearic Islands (reference FPI04 43126005Q) and J.M.A. by a contract of the Ramón y Cajal program of the Spanish Ministry of Economy and Competitiveness.
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0304377016300213
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGarcias Bonet, Neusen
dc.contributor.authorArrieta, Jesusen
dc.contributor.authorDuarte, Carlos M.en
dc.contributor.authorMarbà, Núriaen
dc.date.accessioned2016-03-10T13:12:37Zen
dc.date.available2016-03-10T13:12:37Zen
dc.date.issued2016-03-09en
dc.identifier.citationNitrogen-fixing bacteria in Mediterranean seagrass (Posidonia oceanica) roots 2016 Aquatic Botanyen
dc.identifier.issn03043770en
dc.identifier.doi10.1016/j.aquabot.2016.03.002en
dc.identifier.urihttp://hdl.handle.net/10754/601136en
dc.description.abstractBiological nitrogen fixation by diazotrophic bacteria in seagrass rhizosphere and leaf epiphytic community is an important source of nitrogen required for plant growth. However, the presence of endophytic diazotrophs remains unclear in seagrass tissues. Here, we assess the presence, diversity and taxonomy of nitrogen-fixing bacteria within surface-sterilized roots of Posidonia oceanica. Moreover, we analyze the nitrogen isotopic signature of seagrass tissues in order to notice atmospheric nitrogen fixation. We detected nitrogen-fixing bacteria by nifH gene amplification in 13 out of the 78 roots sampled, corresponding to 9 locations out of 26 meadows. We detected two different types of bacterial nifH sequences associated with P. oceanica roots, which were closely related to sequences previously isolated from the rhizosphere of a salt marsh cord grass and a putative anaerobe. Nitrogen content of seagrass tissues showed low isotopic signatures in all the sampled meadows, pointing out the atmospheric origin of the assimilated nitrogen by seagrasses. However, this was not related with the presence of endophytic nitrogen fixers, suggesting the nitrogen fixation occurring in rhizosphere and in the epiphytic community could be an important source of nitrogen for P. oceanica. The low diversity of nitrogen-fixing bacteria reported here suggests species-specific relationships between diazotrophs and P. oceanica, revealing possible symbiotic interactions that could play a major role in nitrogen acquisition by seagrasses in oligotrophic environments where they form lush meadows.en
dc.description.sponsorshipThis study was funded by the projects MEDEICG and ESTRESX of the Spanish Marine Science and Technology Program (CTM2009-07013, CTM2012-32603). N.G.B. was supported by a PhD grant from the Government of Balearic Islands (reference FPI04 43126005Q) and J.M.A. by a contract of the Ramón y Cajal program of the Spanish Ministry of Economy and Competitiveness.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0304377016300213en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Aquatic Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Botany, 9 March 2016. DOI: 10.1016/j.aquabot.2016.03.002en
dc.subjectdiazotrophyen
dc.subjectnitrogen fixationen
dc.subjectseagrass-bacteria interactionen
dc.subjectendophytesen
dc.titleNitrogen-fixing bacteria in Mediterranean seagrass (Posidonia oceanica) rootsen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalAquatic Botanyen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Global Change Research, Institut Mediterrani d'Estudis Avançats, IMEDEA (CSIC-UIB), Esporles, Spainen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorGarcias Bonet, Neusen
kaust.authorArrieta, Jesusen
kaust.authorDuarte, Carlos M.en
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