Cytotoxic effects of nickel nanowires in human fibroblasts

Handle URI:
http://hdl.handle.net/10754/601122
Title:
Cytotoxic effects of nickel nanowires in human fibroblasts
Authors:
Felix Servin, Laura P.; Perez, Jose E. ( 0000-0002-2206-0034 ) ; Contreras, Maria F. ( 0000-0001-6239-5325 ) ; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
The increasing interest in the use of magnetic nanostructures for biomedical applications necessitates rigorous studies to be carried out in order to determine their potential toxicity. This work attempts to elucidate the cytotoxic effects of nickel nanowires (NWs) in human fibroblasts WI-38 by a colorimetric assay (MTT) under two different parameters: NW concentration and exposure time. This was complemented with TEM and confocal images to assess the NWs internalization and to identify any changes in the cell morphology. Ni NWs were fabricated by electrodeposition using porous alumina templates. Energy dispersive X-Ray analysis, scanning electron microscopy and transmission electron microscopy imaging were used for NW characterization. The results showed decreased cell metabolic activity for incubation times longer than 24 hours and no negative effects for exposure times shorter than that. The cytotoxicity effects for human fibroblasts were then compared with those reported for HCT 116 cells, and the findings point out that it is relevant to consider the cellular size. In addition, the present study compares the toxic effects of equivalent amounts of nickel in the form of its salt to those of NWs and shows that the NWs are more toxic than the salts. Internalized NWs were found in vesicles inside of the cells where their presence induced inflammation of the endoplasmic reticulum.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Citation:
Cytotoxic effects of nickel nanowires in human fibroblasts 2016 Toxicology Reports
Publisher:
Elsevier BV
Journal:
Toxicology Reports
Issue Date:
9-Mar-2016
DOI:
10.1016/j.toxrep.2016.03.004
Type:
Article
ISSN:
22147500
Sponsors:
Research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S2214750016300245
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorFelix Servin, Laura P.en
dc.contributor.authorPerez, Jose E.en
dc.contributor.authorContreras, Maria F.en
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2016-03-10T13:14:06Zen
dc.date.available2016-03-10T13:14:06Zen
dc.date.issued2016-03-09en
dc.identifier.citationCytotoxic effects of nickel nanowires in human fibroblasts 2016 Toxicology Reportsen
dc.identifier.issn22147500en
dc.identifier.doi10.1016/j.toxrep.2016.03.004en
dc.identifier.urihttp://hdl.handle.net/10754/601122en
dc.description.abstractThe increasing interest in the use of magnetic nanostructures for biomedical applications necessitates rigorous studies to be carried out in order to determine their potential toxicity. This work attempts to elucidate the cytotoxic effects of nickel nanowires (NWs) in human fibroblasts WI-38 by a colorimetric assay (MTT) under two different parameters: NW concentration and exposure time. This was complemented with TEM and confocal images to assess the NWs internalization and to identify any changes in the cell morphology. Ni NWs were fabricated by electrodeposition using porous alumina templates. Energy dispersive X-Ray analysis, scanning electron microscopy and transmission electron microscopy imaging were used for NW characterization. The results showed decreased cell metabolic activity for incubation times longer than 24 hours and no negative effects for exposure times shorter than that. The cytotoxicity effects for human fibroblasts were then compared with those reported for HCT 116 cells, and the findings point out that it is relevant to consider the cellular size. In addition, the present study compares the toxic effects of equivalent amounts of nickel in the form of its salt to those of NWs and shows that the NWs are more toxic than the salts. Internalized NWs were found in vesicles inside of the cells where their presence induced inflammation of the endoplasmic reticulum.en
dc.description.sponsorshipResearch reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST).en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S2214750016300245en
dc.rightsArchived with thanks to Toxicology Reports. Under a Creative Commons license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectcytotoxicityen
dc.subjectnanotechnologyen
dc.subjectnanowiresen
dc.subjectmagneticen
dc.subjecthuman fibroblastsen
dc.titleCytotoxic effects of nickel nanowires in human fibroblastsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalToxicology Reportsen
dc.eprint.versionPost-printen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorFelix Servin, Laura P.en
kaust.authorPerez, Jose E.en
kaust.authorContreras, Maria F.en
kaust.authorRavasi, Timothyen
kaust.authorKosel, Jürgenen
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