Delta-proteobacterial SAR324 group in hydrothermal plumes on the South Mid-Atlantic Ridge

Handle URI:
http://hdl.handle.net/10754/600988
Title:
Delta-proteobacterial SAR324 group in hydrothermal plumes on the South Mid-Atlantic Ridge
Authors:
Cao, Huiluo; Dong, Chunming; Bougouffa, Salim ( 0000-0001-9218-6452 ) ; Li, Jiangtao; Zhang, Weipeng; Shao, Zongze; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Qian, Pei-Yuan
Abstract:
In the dark ocean, the SAR324 group of Delta-proteobacteria has been associated with a chemolithotrophic lifestyle. However, their electron transport chain for energy generation and information system has not yet been well characterized. In the present study, four SAR324 draft genomes were extracted from metagenomes sampled from hydrothermal plumes in the South Mid-Atlantic Ridge. We describe novel electron transport chain components in the SAR324 group, particularly the alternative complex III, which is involved in energy generation. Moreover, we propose that the C-type cytochrome, for example the C553, may play a novel role in electron transfer, adding to our knowledge regarding the energy generation process in the SAR324 cluster. The central carbon metabolism in the described SAR324 genomes exhibits several new features other than methanotrophy e.g. aromatic compound degradation. This suggests that methane oxidation may not be the main central carbon metabolism component in SAR324 cluster bacteria. The reductive acetyl-CoA pathway may potentially be essential in carbon fixation due to the absence of components from the Calvin-Benson cycle. Our study provides insight into the role of recombination events in shaping the genome of the SAR324 group based on a larger number of repeat regions observed, which has been overlooked thus far.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Citation:
Delta-proteobacterial SAR324 group in hydrothermal plumes on the South Mid-Atlantic Ridge 2016, 6:22842 Scientific Reports
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
8-Mar-2016
DOI:
10.1038/srep22842
Type:
Article
ISSN:
2045-2322
Sponsors:
This study was supported by grants from the National Basic Research Program of China (973 Program, No: 2012CB417304) and the China Ocean Mineral Resources R & D Association (COMRRDA/12.SC02) (DY125-15-R-01) to Pei-Yuan Qian, and the State Key Laboratory of Marine Geology, Tongji University (MGK1401) to Huiluo Cao. We thank the R/V “Da-Yang Yi-Hao” crews for their assistance with the sample collections. Salim Bougouffa was supported by a SABIC Postdoctoral Fellowship. Vladimir Bajic was supported by a KAUST Base Research Fund.
Additional Links:
http://www.nature.com/articles/srep22842
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorCao, Huiluoen
dc.contributor.authorDong, Chunmingen
dc.contributor.authorBougouffa, Salimen
dc.contributor.authorLi, Jiangtaoen
dc.contributor.authorZhang, Weipengen
dc.contributor.authorShao, Zongzeen
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2016-03-09T13:21:54Zen
dc.date.available2016-03-09T13:21:54Zen
dc.date.issued2016-03-08en
dc.identifier.citationDelta-proteobacterial SAR324 group in hydrothermal plumes on the South Mid-Atlantic Ridge 2016, 6:22842 Scientific Reportsen
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep22842en
dc.identifier.urihttp://hdl.handle.net/10754/600988en
dc.description.abstractIn the dark ocean, the SAR324 group of Delta-proteobacteria has been associated with a chemolithotrophic lifestyle. However, their electron transport chain for energy generation and information system has not yet been well characterized. In the present study, four SAR324 draft genomes were extracted from metagenomes sampled from hydrothermal plumes in the South Mid-Atlantic Ridge. We describe novel electron transport chain components in the SAR324 group, particularly the alternative complex III, which is involved in energy generation. Moreover, we propose that the C-type cytochrome, for example the C553, may play a novel role in electron transfer, adding to our knowledge regarding the energy generation process in the SAR324 cluster. The central carbon metabolism in the described SAR324 genomes exhibits several new features other than methanotrophy e.g. aromatic compound degradation. This suggests that methane oxidation may not be the main central carbon metabolism component in SAR324 cluster bacteria. The reductive acetyl-CoA pathway may potentially be essential in carbon fixation due to the absence of components from the Calvin-Benson cycle. Our study provides insight into the role of recombination events in shaping the genome of the SAR324 group based on a larger number of repeat regions observed, which has been overlooked thus far.en
dc.description.sponsorshipThis study was supported by grants from the National Basic Research Program of China (973 Program, No: 2012CB417304) and the China Ocean Mineral Resources R & D Association (COMRRDA/12.SC02) (DY125-15-R-01) to Pei-Yuan Qian, and the State Key Laboratory of Marine Geology, Tongji University (MGK1401) to Huiluo Cao. We thank the R/V “Da-Yang Yi-Hao” crews for their assistance with the sample collections. Salim Bougouffa was supported by a SABIC Postdoctoral Fellowship. Vladimir Bajic was supported by a KAUST Base Research Fund.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/articles/srep22842en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleDelta-proteobacterial SAR324 group in hydrothermal plumes on the South Mid-Atlantic Ridgeen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, Chinaen
dc.contributor.institutionKey Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State of Oceanic Administration, Xiamen, Chinaen
dc.contributor.institutionState Key Laboratory of Marine Geology, Tongji University, Shanghai, Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorBougouffa, Salimen
kaust.authorBajic, Vladimir B.en
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