miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria

Handle URI:
http://hdl.handle.net/10754/600684
Title:
miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria
Authors:
Liew, Yi Jin ( 0000-0003-2553-8870 ) ; Ryu, Tae Woo; Aranda, Manuel ( 0000-0001-6673-016X ) ; Ravasi, Timothy ( 0000-0002-9950-465X )
Abstract:
MicroRNAs (miRNAs) are small regulatory RNAs that are involved in many biological process in eukaryotes. They play a crucial role in modulating genetic expression of their targets, which makes them integral components of transcriptional regulatory networks. As sponges (phylum Porifera) are commonly considered the most basal metazoan, the in-depth capture of miRNAs from these organisms provides additional clues to the evolution of miRNA families in metazoans. Here, we identified the core proteins involved in the biogenesis of miRNAs, and obtained evidence for bona fide miRNA sequences for two marine sponges Stylissa carteri and Xestospongia testudinaria (11 and 19 respectively). Our analysis identified several miRNAs that are conserved amongst demosponges, and revealed that all of the novel miRNAs identified in these two species are specific to the class Demospongiae.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC); KAUST Environmental Epigenetic Program (KEEP)
Citation:
miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria 2016, 11 (2):e0149080 PLOS ONE
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
12-Feb-2016
DOI:
10.1371/journal.pone.0149080
Type:
Article
ISSN:
1932-6203
Sponsors:
We thank Annika Haywood, Feras Lafi and Holly Bream for extracting the RNA used for sequencing, as well as two anonymous reviewers for their invaluable suggestions. This project was supported by the King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0149080
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Reef Genomics, part of the Global Ocean Genome Project

Full metadata record

DC FieldValue Language
dc.contributor.authorLiew, Yi Jinen
dc.contributor.authorRyu, Tae Wooen
dc.contributor.authorAranda, Manuelen
dc.contributor.authorRavasi, Timothyen
dc.date.accessioned2016-03-07T07:55:23Zen
dc.date.available2016-03-07T07:55:23Zen
dc.date.issued2016-02-12en
dc.identifier.citationmiRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria 2016, 11 (2):e0149080 PLOS ONEen
dc.identifier.issn1932-6203en
dc.identifier.doi10.1371/journal.pone.0149080en
dc.identifier.urihttp://hdl.handle.net/10754/600684en
dc.description.abstractMicroRNAs (miRNAs) are small regulatory RNAs that are involved in many biological process in eukaryotes. They play a crucial role in modulating genetic expression of their targets, which makes them integral components of transcriptional regulatory networks. As sponges (phylum Porifera) are commonly considered the most basal metazoan, the in-depth capture of miRNAs from these organisms provides additional clues to the evolution of miRNA families in metazoans. Here, we identified the core proteins involved in the biogenesis of miRNAs, and obtained evidence for bona fide miRNA sequences for two marine sponges Stylissa carteri and Xestospongia testudinaria (11 and 19 respectively). Our analysis identified several miRNAs that are conserved amongst demosponges, and revealed that all of the novel miRNAs identified in these two species are specific to the class Demospongiae.en
dc.description.sponsorshipWe thank Annika Haywood, Feras Lafi and Holly Bream for extracting the RNA used for sequencing, as well as two anonymous reviewers for their invaluable suggestions. This project was supported by the King Abdullah University of Science and Technology (KAUST).en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0149080en
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://creativecommons.org/licenses/by/4.0/en
dc.titlemiRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinariaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentKAUST Environmental Epigenetic Program (KEEP)en
dc.identifier.journalPLoS ONEen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorLiew, Yi Jinen
kaust.authorRyu, Tae Wooen
kaust.authorAranda, Manuelen
kaust.authorRavasi, Timothyen
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