The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip

Handle URI:
http://hdl.handle.net/10754/600264
Title:
The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip
Authors:
Lin, Ran; Chang, Donald C.; Lee, Yi Kuen
Abstract:
Electroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (C CD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and C CD. © 2012 IEEE.
Citation:
Lin R, Chang DC, Lee Y-K (2012) The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip. 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). Available: http://dx.doi.org/10.1109/nems.2012.6196744.
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
KAUST Grant Number:
SA-C0040; UK-COOI6
Conference/Event name:
7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Issue Date:
Mar-2012
DOI:
10.1109/nems.2012.6196744
Type:
Conference Paper
ISBN:
9781467311243
Sponsors:
The research was sponsored by a grant from Hong Kong Research Grants Council (Grant Ref. No. 615907) and by an award from the King Abdullah University of Science and Technology (KAUST Award No. SA-C0040/UK-COOI6).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorLin, Ranen
dc.contributor.authorChang, Donald C.en
dc.contributor.authorLee, Yi Kuenen
dc.date.accessioned2016-02-28T08:00:17Zen
dc.date.available2016-02-28T08:00:17Zen
dc.date.issued2012-03en
dc.identifier.citationLin R, Chang DC, Lee Y-K (2012) The effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chip. 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). Available: http://dx.doi.org/10.1109/nems.2012.6196744.en
dc.identifier.isbn9781467311243en
dc.identifier.doi10.1109/nems.2012.6196744en
dc.identifier.urihttp://hdl.handle.net/10754/600264en
dc.description.abstractElectroendocytosis (EED) is a pulsed-electric-field (PEF) induced endocytosis, facilitating cells uptake molecules through nanometer-sized EED vesicles. We herein investigate the effect of a chemical inhibitor, Cytochalasin D (CD) on the actin-filaments (F-Actin) behavior of single-cell EED. The CD concentration (C CD) can control the depolymerization of F-actin. A multi-chamber micro cell chip was fabricated to study the EED under different conditions. Large-scale single-cell data demonstrated EED highly depends on both electric field and C CD. © 2012 IEEE.en
dc.description.sponsorshipThe research was sponsored by a grant from Hong Kong Research Grants Council (Grant Ref. No. 615907) and by an award from the King Abdullah University of Science and Technology (KAUST Award No. SA-C0040/UK-COOI6).en
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.subjectActin filamenten
dc.subjectCytochalasin Den
dc.subjectElectroendocytosisen
dc.subjectMicro Cell Chipen
dc.titleThe effect of Cytochalasin D on F-Actin behavior of single-cell electroendocytosis using multi-chamber micro cell chipen
dc.typeConference Paperen
dc.identifier.journal2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)en
dc.conference.date5 March 2012 through 8 March 2012en
dc.conference.name7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012en
dc.conference.locationKyotoen
dc.contributor.institutionBioengineering Graduate Program, HKUST, Kowloon, Hong Kongen
dc.contributor.institutionDept. of Mechanical Engineering, HKUST, Kowloon, Hong Kongen
dc.contributor.institutionDivision of Life Science, HKUST, Kowloon, Hong Kongen
kaust.authorLee, Yi-Kuenen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-COOI6en
kaust.grant.fundedcenterKAUST-HKUST Micro/Nanofluidic Joint Laboratoryen
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