Improved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trapping

Handle URI:
http://hdl.handle.net/10754/600255
Title:
Improved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trapping
Authors:
Li, Ming; Li, Shunbo; Cao, Wenbin; Li, Weihua; Wen, Weijia; Alici, Gursel
Abstract:
This article presents a dielectrophoresis (DEP)-based microfluidic device with the three-dimensional (3D) microelectrode configuration for concentrating and separating particles in a continuous throughflow. The 3D electrode structure, where microelectrode array are patterned on both the top and bottom surfaces of the microchannel, is composed of three units: focusing, aligning and trapping. As particles flowing through the microfluidic channel, they are firstly focused and aligned by the funnel-shaped and parallel electrode array, respectively, before being captured at the trapping unit due to negative DEP force. For a mixture of two particle populations of different sizes or dielectric properties, with a careful selection of suspending medium and applied field, the population exhibits stronger negative DEP manipulated by the microelectrode array and, therefore, separated from the other population which is easily carried away toward the outlet due to hydrodynamic force. The functionality of the proposed microdevice was verified by concentrating different-sized polystyrene (PS) microparticles and yeast cells dynamically flowing in the microchannel. Moreover, separation based on size and dielectric properties was achieved by sorting PS microparticles, and isolating 5 μm PS particles from yeast cells, respectively. The performance of the proposed micro-concentrator and separator was also studied, including the threshold voltage at which particles begin to be trapped, variation of cell-trapping efficiency with respect to the applied voltage and flow rate, and the efficiency of separation experiments. The proposed microdevice has various advantages, including multi-functionality, improved manipulation efficiency and throughput, easy fabrication and operation, etc., which shows a great potential for biological, chemical and medical applications. © 2012 Springer-Verlag Berlin Heidelberg.
Citation:
Li M, Li S, Cao W, Li W, Wen W, et al. (2012) Improved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trapping. Microfluid Nanofluid 14: 527–539. Available: http://dx.doi.org/10.1007/s10404-012-1071-y.
Publisher:
Springer Nature
Journal:
Microfluidics and Nanofluidics
Issue Date:
18-Oct-2012
DOI:
10.1007/s10404-012-1071-y
Type:
Article
ISSN:
1613-4982; 1613-4990
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Mingen
dc.contributor.authorLi, Shunboen
dc.contributor.authorCao, Wenbinen
dc.contributor.authorLi, Weihuaen
dc.contributor.authorWen, Weijiaen
dc.contributor.authorAlici, Gurselen
dc.date.accessioned2016-02-28T08:00:07Zen
dc.date.available2016-02-28T08:00:07Zen
dc.date.issued2012-10-18en
dc.identifier.citationLi M, Li S, Cao W, Li W, Wen W, et al. (2012) Improved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trapping. Microfluid Nanofluid 14: 527–539. Available: http://dx.doi.org/10.1007/s10404-012-1071-y.en
dc.identifier.issn1613-4982en
dc.identifier.issn1613-4990en
dc.identifier.doi10.1007/s10404-012-1071-yen
dc.identifier.urihttp://hdl.handle.net/10754/600255en
dc.description.abstractThis article presents a dielectrophoresis (DEP)-based microfluidic device with the three-dimensional (3D) microelectrode configuration for concentrating and separating particles in a continuous throughflow. The 3D electrode structure, where microelectrode array are patterned on both the top and bottom surfaces of the microchannel, is composed of three units: focusing, aligning and trapping. As particles flowing through the microfluidic channel, they are firstly focused and aligned by the funnel-shaped and parallel electrode array, respectively, before being captured at the trapping unit due to negative DEP force. For a mixture of two particle populations of different sizes or dielectric properties, with a careful selection of suspending medium and applied field, the population exhibits stronger negative DEP manipulated by the microelectrode array and, therefore, separated from the other population which is easily carried away toward the outlet due to hydrodynamic force. The functionality of the proposed microdevice was verified by concentrating different-sized polystyrene (PS) microparticles and yeast cells dynamically flowing in the microchannel. Moreover, separation based on size and dielectric properties was achieved by sorting PS microparticles, and isolating 5 μm PS particles from yeast cells, respectively. The performance of the proposed micro-concentrator and separator was also studied, including the threshold voltage at which particles begin to be trapped, variation of cell-trapping efficiency with respect to the applied voltage and flow rate, and the efficiency of separation experiments. The proposed microdevice has various advantages, including multi-functionality, improved manipulation efficiency and throughput, easy fabrication and operation, etc., which shows a great potential for biological, chemical and medical applications. © 2012 Springer-Verlag Berlin Heidelberg.en
dc.publisherSpringer Natureen
dc.subject3D electrodesen
dc.subjectDielectrophoresisen
dc.subjectMicrofluidicsen
dc.subjectParticle concentrationen
dc.subjectParticle separationen
dc.titleImproved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trappingen
dc.typeArticleen
dc.identifier.journalMicrofluidics and Nanofluidicsen
dc.contributor.institutionSchool of Mechanical, Materials and Mechatronic Engineering, University of Wollongong, Wollongong, NSW 2522, Australiaen
dc.contributor.institutionDepartment of Physics, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
kaust.authorCao, Wenbinen
kaust.authorWen, Weijiaen
kaust.grant.fundedcenterKAUST-HKUST Micro/Nanofluidic Joint Laboratoryen
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