Electroporation of micro-droplet encapsulated HeLa cells in oil phase

Handle URI:
http://hdl.handle.net/10754/600252
Title:
Electroporation of micro-droplet encapsulated HeLa cells in oil phase
Authors:
Xiao, Kang; Zhang, Mengying; Chen, Shuyu; Wang, Limu; Chang, Donald Choy; Wen, Weijia
Abstract:
Electroporation (EP) is a method widely used to introduce foreign genes, drugs or dyes into cells by permeabilizing the plasma membrane with an external electric field. A variety of microfluidic EP devices have been reported so far. However, further integration of prior and posterior EP processes turns out to be very complicated, mainly due to the difficulty of developing an efficient method for precise manipulation of cells in microfluidics. In this study, by means of a T-junction structure within a delicate microfluidic device, we encapsulated HeLa cells in micro-droplet of poration medium in oil phase before EP, which has two advantages: (i) precise control of cell-encapsulating droplets in oil phase is much easier than the control of cell populations or individuals in aqueous buffers; (ii) this can minimize the electrochemical reactions on the electrodes. Finally, we successfully introduced fluorescent dyes into the micro-droplet encapsulated HeLa cells in oil phase. Our results reflected a novel way to realize the integrated biomicrofluidic system for EP. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Citation:
Xiao K, Zhang M, Chen S, Wang L, Chang DC, et al. (2010) Electroporation of micro-droplet encapsulated HeLa cells in oil phase. ELECTROPHORESIS 31: 3175–3180. Available: http://dx.doi.org/10.1002/elps.201000155.
Publisher:
Wiley-Blackwell
Journal:
ELECTROPHORESIS
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
27-Aug-2010
DOI:
10.1002/elps.201000155
PubMed ID:
20803502
Type:
Article
ISSN:
0173-0835
Sponsors:
The authors acknowledge the financial support provided by the Hong Kong Research Grants Council Grant No. HKUST 660207. This article is based on the work partially supported by Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST). The authors thank Dr. Chih-Kuan Tung and Jinbo Wu for their discussions with them.
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorXiao, Kangen
dc.contributor.authorZhang, Mengyingen
dc.contributor.authorChen, Shuyuen
dc.contributor.authorWang, Limuen
dc.contributor.authorChang, Donald Choyen
dc.contributor.authorWen, Weijiaen
dc.date.accessioned2016-02-28T08:00:03Zen
dc.date.available2016-02-28T08:00:03Zen
dc.date.issued2010-08-27en
dc.identifier.citationXiao K, Zhang M, Chen S, Wang L, Chang DC, et al. (2010) Electroporation of micro-droplet encapsulated HeLa cells in oil phase. ELECTROPHORESIS 31: 3175–3180. Available: http://dx.doi.org/10.1002/elps.201000155.en
dc.identifier.issn0173-0835en
dc.identifier.pmid20803502en
dc.identifier.doi10.1002/elps.201000155en
dc.identifier.urihttp://hdl.handle.net/10754/600252en
dc.description.abstractElectroporation (EP) is a method widely used to introduce foreign genes, drugs or dyes into cells by permeabilizing the plasma membrane with an external electric field. A variety of microfluidic EP devices have been reported so far. However, further integration of prior and posterior EP processes turns out to be very complicated, mainly due to the difficulty of developing an efficient method for precise manipulation of cells in microfluidics. In this study, by means of a T-junction structure within a delicate microfluidic device, we encapsulated HeLa cells in micro-droplet of poration medium in oil phase before EP, which has two advantages: (i) precise control of cell-encapsulating droplets in oil phase is much easier than the control of cell populations or individuals in aqueous buffers; (ii) this can minimize the electrochemical reactions on the electrodes. Finally, we successfully introduced fluorescent dyes into the micro-droplet encapsulated HeLa cells in oil phase. Our results reflected a novel way to realize the integrated biomicrofluidic system for EP. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.en
dc.description.sponsorshipThe authors acknowledge the financial support provided by the Hong Kong Research Grants Council Grant No. HKUST 660207. This article is based on the work partially supported by Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST). The authors thank Dr. Chih-Kuan Tung and Jinbo Wu for their discussions with them.en
dc.publisherWiley-Blackwellen
dc.subjectElectroporationen
dc.subjectMicro-dropletsen
dc.subjectMicrofluidicsen
dc.titleElectroporation of micro-droplet encapsulated HeLa cells in oil phaseen
dc.typeArticleen
dc.identifier.journalELECTROPHORESISen
dc.contributor.institutionDepartment of Biology, Hong Kong University of Science and Technology, Kowloon, Hong Kongen
dc.contributor.institutionNanoscience and Nanotechnology Program, Hong Kong University of Science and Technology, Kowloon, Hong Kongen
kaust.authorWen, Weijiaen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.fundedcenterKAUST-HKUST Micro/Nanofluidic Joint Laboratoryen

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