DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips

Handle URI:
http://hdl.handle.net/10754/600249
Title:
DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips
Authors:
Deng, Peigang; Chang, Donald C.; Lee, Yi Kuen; Zhou, Junwei; Li, Gang
Abstract:
Experimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP "phase diagram", which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP "phase diagram". © 2011 IEEE.
Citation:
Deng P, Chang DC, Lee Y-K, Zhou J, Li G (2011) DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips. 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Available: http://dx.doi.org/10.1109/nems.2011.6017304.
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
KAUST Grant Number:
SA-C0040; UK-COOI6
Conference/Event name:
6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Issue Date:
Feb-2011
DOI:
10.1109/nems.2011.6017304
Type:
Conference Paper
ISBN:
9781612847757
Sponsors:
This research is in part on work supported by Award No. SA-C0040/UK-COOI6, made by King Abdullah University of Science and Technology and partially supported by a Hong Kong RGC grant (No. 615907).
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Full metadata record

DC FieldValue Language
dc.contributor.authorDeng, Peigangen
dc.contributor.authorChang, Donald C.en
dc.contributor.authorLee, Yi Kuenen
dc.contributor.authorZhou, Junweien
dc.contributor.authorLi, Gangen
dc.date.accessioned2016-02-28T07:59:59Zen
dc.date.available2016-02-28T07:59:59Zen
dc.date.issued2011-02en
dc.identifier.citationDeng P, Chang DC, Lee Y-K, Zhou J, Li G (2011) DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips. 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Available: http://dx.doi.org/10.1109/nems.2011.6017304.en
dc.identifier.isbn9781612847757en
dc.identifier.doi10.1109/nems.2011.6017304en
dc.identifier.urihttp://hdl.handle.net/10754/600249en
dc.description.abstractExperimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP "phase diagram", which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP "phase diagram". © 2011 IEEE.en
dc.description.sponsorshipThis research is in part on work supported by Award No. SA-C0040/UK-COOI6, made by King Abdullah University of Science and Technology and partially supported by a Hong Kong RGC grant (No. 615907).en
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.subjectbone marrow mesenchymal stem cellen
dc.subjectelectroporationen
dc.subjectEP phase diagramen
dc.subjectmicro electroporation chipen
dc.titleDNA transfection of bone marrow mesenchymal stem cells using micro electroporation chipsen
dc.typeConference Paperen
dc.identifier.journal2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systemsen
dc.conference.date20 February 2011 through 23 February 2011en
dc.conference.name6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011en
dc.conference.locationKaohsiungen
dc.contributor.institutionDepartment of Biology, HKUST, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.institutionStem Cell and Regeneration Program, School of Biomedical Sciences, CUHK, Shatin, NT, Hong Kongen
kaust.authorDeng, Peigangen
kaust.authorLee, Yi-Kuenen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-COOI6en
kaust.grant.fundedcenterKAUST-HKUST Micro/Nanofluidic Joint Laboratoryen
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