Response of cyprid specific genes to natural settlement cues in the barnacle Balanus (=Amphibalanus) amphitrite

Handle URI:
http://hdl.handle.net/10754/600221
Title:
Response of cyprid specific genes to natural settlement cues in the barnacle Balanus (=Amphibalanus) amphitrite
Authors:
Li, Honglei; Thiyagarajan, Vengatesen; Qian, Pei Yuan
Abstract:
Quantitative real-time PCR was used to further our understanding of the molecular processes involved in the attachment and metamorphosis of larval barnacles. We report the effects of natural settlement cues (microbial biofilms and conspecific settlement-inducing factor) on the expression profiles of six barnacle cyprid specific (bcs) genes in cyprids of the barnacle Balanus (=Amphibalanus) amphitrite Darwin. Genes bcs-1 to bcs-5 all showed marked decreases in their expression between initial cyprid attachment and the completion of metamorphosis, whereas bcs-6 showed significant up-regulation. Generally, settlement cues exerted no significant effect on the decreasing trend of bcs-1 to bcs-5 expression during attachment and metamorphosis. However, the expression of bcs-6 increased prior to cyprid attachment in response to both settlement cues. This elevated expression of bcs-6 gene indicates the importance and key regulatory role of this specific gene to larval attachment and metamorphosis in this barnacle species. © 2010 Elsevier B.V. All rights reserved.
Citation:
Li H, Thiyagarajan V, Qian P-Y (2010) Response of cyprid specific genes to natural settlement cues in the barnacle Balanus (=Amphibalanus) amphitrite. Journal of Experimental Marine Biology and Ecology 389: 45–52. Available: http://dx.doi.org/10.1016/j.jembe.2010.03.019.
Publisher:
Elsevier BV
Journal:
Journal of Experimental Marine Biology and Ecology
KAUST Grant Number:
SA-00040; UK-00016
Issue Date:
Jun-2010
DOI:
10.1016/j.jembe.2010.03.019
Type:
Article
ISSN:
0022-0981
Sponsors:
We would like to thank several anonymous referees for their constructive comments on the previous version of this manuscript, particularly on data analysis. This study was supported by an award (SA-00040/UK-00016) of the King Abdullah University of Science and Technology, and the RGC grants (N-HKUST602/09, 662408, and AoE/ P-04/011-II) of the government of the Hong Kong Special Administrative Region to PY Qian. [SS]
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Hongleien
dc.contributor.authorThiyagarajan, Vengatesenen
dc.contributor.authorQian, Pei Yuanen
dc.date.accessioned2016-02-28T07:59:20Zen
dc.date.available2016-02-28T07:59:20Zen
dc.date.issued2010-06en
dc.identifier.citationLi H, Thiyagarajan V, Qian P-Y (2010) Response of cyprid specific genes to natural settlement cues in the barnacle Balanus (=Amphibalanus) amphitrite. Journal of Experimental Marine Biology and Ecology 389: 45–52. Available: http://dx.doi.org/10.1016/j.jembe.2010.03.019.en
dc.identifier.issn0022-0981en
dc.identifier.doi10.1016/j.jembe.2010.03.019en
dc.identifier.urihttp://hdl.handle.net/10754/600221en
dc.description.abstractQuantitative real-time PCR was used to further our understanding of the molecular processes involved in the attachment and metamorphosis of larval barnacles. We report the effects of natural settlement cues (microbial biofilms and conspecific settlement-inducing factor) on the expression profiles of six barnacle cyprid specific (bcs) genes in cyprids of the barnacle Balanus (=Amphibalanus) amphitrite Darwin. Genes bcs-1 to bcs-5 all showed marked decreases in their expression between initial cyprid attachment and the completion of metamorphosis, whereas bcs-6 showed significant up-regulation. Generally, settlement cues exerted no significant effect on the decreasing trend of bcs-1 to bcs-5 expression during attachment and metamorphosis. However, the expression of bcs-6 increased prior to cyprid attachment in response to both settlement cues. This elevated expression of bcs-6 gene indicates the importance and key regulatory role of this specific gene to larval attachment and metamorphosis in this barnacle species. © 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipWe would like to thank several anonymous referees for their constructive comments on the previous version of this manuscript, particularly on data analysis. This study was supported by an award (SA-00040/UK-00016) of the King Abdullah University of Science and Technology, and the RGC grants (N-HKUST602/09, 662408, and AoE/ P-04/011-II) of the government of the Hong Kong Special Administrative Region to PY Qian. [SS]en
dc.publisherElsevier BVen
dc.titleResponse of cyprid specific genes to natural settlement cues in the barnacle Balanus (=Amphibalanus) amphitriteen
dc.typeArticleen
dc.identifier.journalJournal of Experimental Marine Biology and Ecologyen
dc.contributor.institutionSchool of Biological Sciences, The University of Hong Kong, Pok Fu Lam Road, Hong Kongen
kaust.authorLi, Hongleien
kaust.authorQian, Pei-Yuanen
kaust.grant.numberSA-00040en
kaust.grant.numberUK-00016en
kaust.grant.programKAUST Global Collaborative Research Programen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.