First study on gene expression of cement proteins and potential adhesion-related genes of a membranous-based barnacle as revealed from Next-Generation Sequencing technology

Handle URI:
http://hdl.handle.net/10754/600218
Title:
First study on gene expression of cement proteins and potential adhesion-related genes of a membranous-based barnacle as revealed from Next-Generation Sequencing technology
Authors:
Lin, Hsiu Chin; Wong, Yue Him; Tsang, Ling Ming; Chu, Ka Hou; Qian, Pei Yuan; Chan, Benny K K
Abstract:
This is the first study applying Next-Generation Sequencing (NGS) technology to survey the kinds, expression location, and pattern of adhesion-related genes in a membranous-based barnacle. A total of 77,528,326 and 59,244,468 raw sequence reads of total RNA were generated from the prosoma and the basis of Tetraclita japonica formosana, respectively. In addition, 55,441 and 67,774 genes were further assembled and analyzed. The combined sequence data from both body parts generates a total of 79,833 genes of which 47.7% were shared. Homologues of barnacle cement proteins - CP-19K, -52K, and -100K - were found and all were dominantly expressed at the basis where the cement gland complex is located. This is the main area where transcripts of cement proteins and other potential adhesion-related genes were detected. The absence of another common barnacle cement protein, CP-20K, in the adult transcriptome suggested a possible life-stage restricted gene function and/or a different mechanism in adhesion between membranous-based and calcareous-based barnacles. © 2013 © 2013 Taylor & Francis.
Citation:
Lin H-C, Wong YH, Tsang LM, Chu KH, Qian P-Y, et al. (2013) First study on gene expression of cement proteins and potential adhesion-related genes of a membranous-based barnacle as revealed from Next-Generation Sequencing technology. Biofouling 30: 169–181. Available: http://dx.doi.org/10.1080/08927014.2013.853051.
Publisher:
Informa UK Limited
Journal:
Biofouling
Issue Date:
12-Dec-2013
DOI:
10.1080/08927014.2013.853051
PubMed ID:
24329402
Type:
Article
ISSN:
0892-7014; 1029-2454
Sponsors:
The authors would like to thank P.-C. Tsai (Academia Sinica) for her assistance in specimen collection, and Genomics BioSci & Tech Company in Taiwan for assisting in specimen processing and data analyses. The authors would also like to thank the three anonymous reviewers for their constructive comments. This work was supported by Academia Sinica Career Development Award [AS-98-CDA-L15]; and National Science Council [Grant NSC-99-2621-B-001-007-MY3] to BKK Chan.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorLin, Hsiu Chinen
dc.contributor.authorWong, Yue Himen
dc.contributor.authorTsang, Ling Mingen
dc.contributor.authorChu, Ka Houen
dc.contributor.authorQian, Pei Yuanen
dc.contributor.authorChan, Benny K Ken
dc.date.accessioned2016-02-28T07:59:16Zen
dc.date.available2016-02-28T07:59:16Zen
dc.date.issued2013-12-12en
dc.identifier.citationLin H-C, Wong YH, Tsang LM, Chu KH, Qian P-Y, et al. (2013) First study on gene expression of cement proteins and potential adhesion-related genes of a membranous-based barnacle as revealed from Next-Generation Sequencing technology. Biofouling 30: 169–181. Available: http://dx.doi.org/10.1080/08927014.2013.853051.en
dc.identifier.issn0892-7014en
dc.identifier.issn1029-2454en
dc.identifier.pmid24329402en
dc.identifier.doi10.1080/08927014.2013.853051en
dc.identifier.urihttp://hdl.handle.net/10754/600218en
dc.description.abstractThis is the first study applying Next-Generation Sequencing (NGS) technology to survey the kinds, expression location, and pattern of adhesion-related genes in a membranous-based barnacle. A total of 77,528,326 and 59,244,468 raw sequence reads of total RNA were generated from the prosoma and the basis of Tetraclita japonica formosana, respectively. In addition, 55,441 and 67,774 genes were further assembled and analyzed. The combined sequence data from both body parts generates a total of 79,833 genes of which 47.7% were shared. Homologues of barnacle cement proteins - CP-19K, -52K, and -100K - were found and all were dominantly expressed at the basis where the cement gland complex is located. This is the main area where transcripts of cement proteins and other potential adhesion-related genes were detected. The absence of another common barnacle cement protein, CP-20K, in the adult transcriptome suggested a possible life-stage restricted gene function and/or a different mechanism in adhesion between membranous-based and calcareous-based barnacles. © 2013 © 2013 Taylor & Francis.en
dc.description.sponsorshipThe authors would like to thank P.-C. Tsai (Academia Sinica) for her assistance in specimen collection, and Genomics BioSci & Tech Company in Taiwan for assisting in specimen processing and data analyses. The authors would also like to thank the three anonymous reviewers for their constructive comments. This work was supported by Academia Sinica Career Development Award [AS-98-CDA-L15]; and National Science Council [Grant NSC-99-2621-B-001-007-MY3] to BKK Chan.en
dc.publisherInforma UK Limiteden
dc.subjectcement glanden
dc.subjectcement proteinsen
dc.subjectfoulingen
dc.subjectIllumina sequencingen
dc.subjecttranscriptomeen
dc.titleFirst study on gene expression of cement proteins and potential adhesion-related genes of a membranous-based barnacle as revealed from Next-Generation Sequencing technologyen
dc.typeArticleen
dc.identifier.journalBiofoulingen
dc.contributor.institutionBiodiversity Research Center, Academia Sinica, Taipei 115, Taiwanen
dc.contributor.institutionInstitute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwanen
dc.contributor.institutionSimon F.S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kongen
kaust.authorWong, Yue Himen
kaust.authorQian, Pei-Yuanen
kaust.grant.programKAUST Global Collaborative Research Programen

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.