Why has reversal of the actin-myosin cross-bridge cycle not been observed experimentally?

Handle URI:
http://hdl.handle.net/10754/600194
Title:
Why has reversal of the actin-myosin cross-bridge cycle not been observed experimentally?
Authors:
Loiselle, D. S.; Tran, K.; Crampin, E. J.; Curtin, N. A.
Abstract:
We trace the history of attempts to determine whether the experimentally observed diminution of metabolic energy expenditure when muscles lengthen during active contraction is consistent with reversibility of biochemical reactions and, in particular, with the regeneration of ATP. We note that this scientific endeavor has something of a parallel flavor to it, with both early and more recent experiments exploiting both isolated muscle preparations and exercising human subjects. In tracing this history from the late 19th century to the present, it becomes clear that energy can be (at least transiently) stored in a muscle undergoing an eccentric contraction but that this is unlikely to be due to the regeneration of ATP. A recently developed, thermodynamically constrained model of the cross-bridge cycle provides additional insight into this conclusion. Copyright © 2010 the American Physiological Society.
Citation:
Loiselle DS, Tran K, Crampin EJ, Curtin NA (2010) Why has reversal of the actin-myosin cross-bridge cycle not been observed experimentally? Journal of Applied Physiology 108: 1465–1471. Available: http://dx.doi.org/10.1152/japplphysiol.01198.2009.
Publisher:
American Physiological Society
Journal:
Journal of Applied Physiology
KAUST Grant Number:
KUK-C1-013-04
Issue Date:
4-Feb-2010
DOI:
10.1152/japplphysiol.01198.2009
PubMed ID:
20133436
Type:
Article
ISSN:
8750-7587; 1522-1601
Sponsors:
This work was supported by Grant UOA0607 from the Royal Society of New Zealand Marsden Fund. This publication is based on work (E.J. Crampin) supported in part by Award No KUK-C1-013-04, made by King Abdullah Univ. of Science and Technology (KAUST).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorLoiselle, D. S.en
dc.contributor.authorTran, K.en
dc.contributor.authorCrampin, E. J.en
dc.contributor.authorCurtin, N. A.en
dc.date.accessioned2016-02-28T06:44:56Zen
dc.date.available2016-02-28T06:44:56Zen
dc.date.issued2010-02-04en
dc.identifier.citationLoiselle DS, Tran K, Crampin EJ, Curtin NA (2010) Why has reversal of the actin-myosin cross-bridge cycle not been observed experimentally? Journal of Applied Physiology 108: 1465–1471. Available: http://dx.doi.org/10.1152/japplphysiol.01198.2009.en
dc.identifier.issn8750-7587en
dc.identifier.issn1522-1601en
dc.identifier.pmid20133436en
dc.identifier.doi10.1152/japplphysiol.01198.2009en
dc.identifier.urihttp://hdl.handle.net/10754/600194en
dc.description.abstractWe trace the history of attempts to determine whether the experimentally observed diminution of metabolic energy expenditure when muscles lengthen during active contraction is consistent with reversibility of biochemical reactions and, in particular, with the regeneration of ATP. We note that this scientific endeavor has something of a parallel flavor to it, with both early and more recent experiments exploiting both isolated muscle preparations and exercising human subjects. In tracing this history from the late 19th century to the present, it becomes clear that energy can be (at least transiently) stored in a muscle undergoing an eccentric contraction but that this is unlikely to be due to the regeneration of ATP. A recently developed, thermodynamically constrained model of the cross-bridge cycle provides additional insight into this conclusion. Copyright © 2010 the American Physiological Society.en
dc.description.sponsorshipThis work was supported by Grant UOA0607 from the Royal Society of New Zealand Marsden Fund. This publication is based on work (E.J. Crampin) supported in part by Award No KUK-C1-013-04, made by King Abdullah Univ. of Science and Technology (KAUST).en
dc.publisherAmerican Physiological Societyen
dc.subjectEccentric contractionen
dc.subjectMuscle thermodynamicsen
dc.subjectNegative worken
dc.subjectStretchen
dc.titleWhy has reversal of the actin-myosin cross-bridge cycle not been observed experimentally?en
dc.typeArticleen
dc.identifier.journalJournal of Applied Physiologyen
dc.contributor.institutionUniversity of Auckland, Auckland, New Zealanden
dc.contributor.institutionDepartment of Engineering Science, Auckland, New Zealanden
dc.contributor.institutionNational Heart and Lung Institute, London, United Kingdomen
kaust.grant.numberKUK-C1-013-04en

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