Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids

Handle URI:
http://hdl.handle.net/10754/600112
Title:
Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids
Authors:
Dash, Swagatika; Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Zhang, Yu; Qian, Pei Yuan
Abstract:
Biofouling is ubiquitous in marine environments, and the barnacle Balanus amphitrite is one of the most recalcitrant and aggressive biofoulers in tropical waters. Several natural antifoulants that were claimed to be non-toxic have been isolated in recent years, although the mechanism by which they inhibit fouling is yet to be investigated. Poly-ether B has shown promise in the non-toxic inhibition of larval barnacle attachment. Hence, in this study, multiplex two-dimensional electrophoresis (2-DE) was applied in conjunction with mass spectrometry to investigate the effects of poly-ether B on barnacle larvae at the molecular level. The cyprid proteome response to poly-ether B treatment was analyzed at the total proteome and phosphoproteome levels, with 65 protein and 19 phosphoprotein spots found to be up- or down-regulated. The proteins were found to be related to energy-metabolism, oxidative stress, and molecular chaperones, thus indicating that poly-ether B may interfere with the redox-regulatory mechanisms governing the settlement of barnacle larvae. The results of this study demonstrate the usefulness of the proteomic technique in revealing the working mechanisms of antifouling compounds. © 2012 Copyright Taylor and Francis Group, LLC.
Citation:
Dash S, Chandramouli KH, Zhang Y, Qian P-Y (2012) Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids . Biofouling 28: 405–415. Available: http://dx.doi.org/10.1080/08927014.2012.679731.
Publisher:
Informa UK Limited
Journal:
Biofouling
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
Apr-2012
DOI:
10.1080/08927014.2012.679731
PubMed ID:
22519465
Type:
Article
ISSN:
0892-7014; 1029-2454
Sponsors:
The authors thank Ms Cherry Kwan for proof reading this manuscript, Dr Wu Huixiang for her kind help with the barnacle culture, Dr Wang Yong for help in the preparation of the figures and Dr Ying Xu for her helpful suggestions. The study was supported by an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology (KAUST), a grant from the China Ocean Mineral Resources Research and Development Association (DY125-15-T-02), and two grants (N_HKUST602/09 and AoE/P-393 04/04-2-II) from the Hong Kong Research Grants Council (RGC) awarded to PY Qian.
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorDash, Swagatikaen
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorZhang, Yuen
dc.contributor.authorQian, Pei Yuanen
dc.date.accessioned2016-02-28T07:59:08Zen
dc.date.available2016-02-28T07:59:08Zen
dc.date.issued2012-04en
dc.identifier.citationDash S, Chandramouli KH, Zhang Y, Qian P-Y (2012) Effects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cyprids . Biofouling 28: 405–415. Available: http://dx.doi.org/10.1080/08927014.2012.679731.en
dc.identifier.issn0892-7014en
dc.identifier.issn1029-2454en
dc.identifier.pmid22519465en
dc.identifier.doi10.1080/08927014.2012.679731en
dc.identifier.urihttp://hdl.handle.net/10754/600112en
dc.description.abstractBiofouling is ubiquitous in marine environments, and the barnacle Balanus amphitrite is one of the most recalcitrant and aggressive biofoulers in tropical waters. Several natural antifoulants that were claimed to be non-toxic have been isolated in recent years, although the mechanism by which they inhibit fouling is yet to be investigated. Poly-ether B has shown promise in the non-toxic inhibition of larval barnacle attachment. Hence, in this study, multiplex two-dimensional electrophoresis (2-DE) was applied in conjunction with mass spectrometry to investigate the effects of poly-ether B on barnacle larvae at the molecular level. The cyprid proteome response to poly-ether B treatment was analyzed at the total proteome and phosphoproteome levels, with 65 protein and 19 phosphoprotein spots found to be up- or down-regulated. The proteins were found to be related to energy-metabolism, oxidative stress, and molecular chaperones, thus indicating that poly-ether B may interfere with the redox-regulatory mechanisms governing the settlement of barnacle larvae. The results of this study demonstrate the usefulness of the proteomic technique in revealing the working mechanisms of antifouling compounds. © 2012 Copyright Taylor and Francis Group, LLC.en
dc.description.sponsorshipThe authors thank Ms Cherry Kwan for proof reading this manuscript, Dr Wu Huixiang for her kind help with the barnacle culture, Dr Wang Yong for help in the preparation of the figures and Dr Ying Xu for her helpful suggestions. The study was supported by an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology (KAUST), a grant from the China Ocean Mineral Resources Research and Development Association (DY125-15-T-02), and two grants (N_HKUST602/09 and AoE/P-393 04/04-2-II) from the Hong Kong Research Grants Council (RGC) awarded to PY Qian.en
dc.publisherInforma UK Limiteden
dc.subject2DEen
dc.subjectantifoulingen
dc.subjectbarnacleen
dc.subjectpoly-etheren
dc.subjectproteomeen
dc.titleEffects of poly-ether B on proteome and phosphoproteome expression in biofouling Balanus amphitrite cypridsen
dc.typeArticleen
dc.identifier.journalBiofoulingen
dc.contributor.institutionHong Kong University of Science and Technology, Hong Kong, Chinaen
kaust.authorDash, Swagatikaen
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorZhang, Yuen
kaust.authorQian, Pei-Yuanen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.programKAUST Global Collaborative Research Programen
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