Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite

Handle URI:
http://hdl.handle.net/10754/600111
Title:
Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite
Authors:
Zhang, Yu; Xu, Ying; Arellano, Shawn M.; Xiao, Kang; Qian, Pei Yuan
Abstract:
The barnacle Balanus amphitrite (=Amphibalanus amphitrite) is a major marine biofouling invertebrate worldwide. It has a complex life cycle during which the larva (called a nauplius) molts six times before transforming into the cyprid stage. The cyprid stage in B. amphitrite is the critical stage for the larval decision to attach and metamorphose. In this study, proteome and phosphoproteome alterations during cyprid development/aging and upon treatment with the antifouling agent butenolide were examined with a two-dimensional electrophoresis (2-DE) multiplexed fluorescent staining approach. Optimized protein separation strategies, including solution-phase isoelectric fractionation and narrow-pH-range 2-DE, were used in a proteomic analysis. Our results show that the differential regulation of the target proteins is highly dynamic on the levels of both protein expression and posttranslational modification. Two groups of proteins, stress-associated and energy metabolism-related proteins, are differentially expressed during cyprid development. Comparison of the control and treatment groups suggests that butenolide exerts its effects by sustaining the expression levels of these proteins. Altogether, our data suggest that proteins involved in stress regulation and energy metabolism play crucial roles in regulating larval attachment and metamorphosis of B. amphitrite. © 2010 American Chemical Society.
Citation:
Zhang Y, Xu Y, Arellano SM, Xiao K, Qian P-Y (2010) Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite . Journal of Proteome Research 9: 3146–3157. Available: http://dx.doi.org/10.1021/pr1000384.
Publisher:
American Chemical Society (ACS)
Journal:
Journal of Proteome Research
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
4-Jun-2010
DOI:
10.1021/pr1000384
PubMed ID:
20397722
Type:
Article
ISSN:
1535-3893; 1535-3907
Sponsors:
We thank Dr. Kondethimmanahalli Chandramouli for conducting sample fractionation and data analysis, Prof. Donald Choy Chang for providing the Sensor-C3 stably expressed HeLa cells (S3), Dr. Huoming Zhang for proof-reading the manuscript, and the rest of the laboratory team for their constructive comments on this work. This work was supported by a grant from the China Ocean Mineral Resources Research and Development Association (COMRRDA06/07.Sc02), an award (SA-C0040/UK-C0016) of the King Abdullah University of Science and Technology, and the RGC grants (N-HKUST602/09, 662408, and AoE/P-04/04-II) of the government of the Hong Kong Special Administrative Region to PY Qian.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Yuen
dc.contributor.authorXu, Yingen
dc.contributor.authorArellano, Shawn M.en
dc.contributor.authorXiao, Kangen
dc.contributor.authorQian, Pei Yuanen
dc.date.accessioned2016-02-28T07:59:06Zen
dc.date.available2016-02-28T07:59:06Zen
dc.date.issued2010-06-04en
dc.identifier.citationZhang Y, Xu Y, Arellano SM, Xiao K, Qian P-Y (2010) Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite . Journal of Proteome Research 9: 3146–3157. Available: http://dx.doi.org/10.1021/pr1000384.en
dc.identifier.issn1535-3893en
dc.identifier.issn1535-3907en
dc.identifier.pmid20397722en
dc.identifier.doi10.1021/pr1000384en
dc.identifier.urihttp://hdl.handle.net/10754/600111en
dc.description.abstractThe barnacle Balanus amphitrite (=Amphibalanus amphitrite) is a major marine biofouling invertebrate worldwide. It has a complex life cycle during which the larva (called a nauplius) molts six times before transforming into the cyprid stage. The cyprid stage in B. amphitrite is the critical stage for the larval decision to attach and metamorphose. In this study, proteome and phosphoproteome alterations during cyprid development/aging and upon treatment with the antifouling agent butenolide were examined with a two-dimensional electrophoresis (2-DE) multiplexed fluorescent staining approach. Optimized protein separation strategies, including solution-phase isoelectric fractionation and narrow-pH-range 2-DE, were used in a proteomic analysis. Our results show that the differential regulation of the target proteins is highly dynamic on the levels of both protein expression and posttranslational modification. Two groups of proteins, stress-associated and energy metabolism-related proteins, are differentially expressed during cyprid development. Comparison of the control and treatment groups suggests that butenolide exerts its effects by sustaining the expression levels of these proteins. Altogether, our data suggest that proteins involved in stress regulation and energy metabolism play crucial roles in regulating larval attachment and metamorphosis of B. amphitrite. © 2010 American Chemical Society.en
dc.description.sponsorshipWe thank Dr. Kondethimmanahalli Chandramouli for conducting sample fractionation and data analysis, Prof. Donald Choy Chang for providing the Sensor-C3 stably expressed HeLa cells (S3), Dr. Huoming Zhang for proof-reading the manuscript, and the rest of the laboratory team for their constructive comments on this work. This work was supported by a grant from the China Ocean Mineral Resources Research and Development Association (COMRRDA06/07.Sc02), an award (SA-C0040/UK-C0016) of the King Abdullah University of Science and Technology, and the RGC grants (N-HKUST602/09, 662408, and AoE/P-04/04-II) of the government of the Hong Kong Special Administrative Region to PY Qian.en
dc.publisherAmerican Chemical Society (ACS)en
dc.subject2-DEen
dc.subjectbarnacleen
dc.subjectIEF sample prefractionationen
dc.subjectlarval developmenten
dc.subjectlarval settlementen
dc.subjectphosphoproteomeen
dc.subjectproteomeen
dc.titleComparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitriteen
dc.typeArticleen
dc.identifier.journalJournal of Proteome Researchen
dc.contributor.institutionHong Kong University of Science and Technology, Hong Kong, Chinaen
kaust.authorZhang, Yuen
kaust.authorXu, Yingen
kaust.authorArellano, Shawn M.en
kaust.authorXiao, Kangen
kaust.authorQian, Pei-Yuanen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.programKAUST Global Collaborative Research Programen

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