Trace methane oxidation and the methane dependency of sulfate reduction in anaerobic granular sludge

Handle URI:
http://hdl.handle.net/10754/600053
Title:
Trace methane oxidation and the methane dependency of sulfate reduction in anaerobic granular sludge
Authors:
Meulepas, Roel J.W.; Jagersma, Christian G.; Zhang, Yu; Petrillo, Michele; Cai, Hengzhe; Buisman, Cees J.N.; Stams, Alfons J.M.; Lens, Piet N.L.
Abstract:
This study investigates the oxidation of labeled methane (CH4) and the CH4 dependence of sulfate reduction in three types of anaerobic granular sludge. In all samples, 13C-labeled CH4 was anaerobically oxidized to 13C-labeled CO2, while net endogenous CH4 production was observed. Labeled-CH4 oxidation rates followed CH4 production rates, and the presence of sulfate hampered both labeled-CH4 oxidation and methanogenesis. Labeled-CH4 oxidation was therefore linked to methanogenesis. This process is referred to as trace CH4 oxidation and has been demonstrated in methanogenic pure cultures. This study shows that the ratio between labeled-CH4 oxidation and methanogenesis is positively affected by the CH4 partial pressure and that this ratio is in methanogenic granular sludge more than 40 times higher than that in pure cultures of methanogens. The CH4 partial pressure also positively affected sulfate reduction and negatively affected methanogenesis: a repression of methanogenesis at elevated CH4 partial pressures confers an advantage to sulfate reducers that compete with methanogens for common substrates, formed from endogenous material. The oxidation of labeled CH 4 and the CH4 dependence of sulfate reduction are thus not necessarily evidence of anaerobic oxidation of CH4 coupled to sulfate reduction. © 2010 Federation of European Microbiological Societies.
Citation:
Meulepas RJW, Jagersma CG, Zhang Y, Petrillo M, Cai H, et al. (2010) Trace methane oxidation and the methane dependency of sulfate reduction in anaerobic granular sludge. FEMS Microbiology Ecology 72: 261–271. Available: http://dx.doi.org/10.1111/j.1574-6941.2010.00849.x.
Publisher:
Oxford University Press (OUP)
Journal:
FEMS Microbiology Ecology
Issue Date:
May-2010
DOI:
10.1111/j.1574-6941.2010.00849.x
PubMed ID:
20337708
Type:
Article
ISSN:
0168-6496; 1574-6941
Sponsors:
This work was part of the Anaerobic Methane Oxidation forSulfate Reduction project (AMethOx for SuRe, numberEETK03044) supported by the Dutch ministries of Economicalaffairs, Education, culture and science and Environmentand special planning as part their EET (Economie, Ecologie,Technologie) program. The research was cofunded by KingAbdullah University of Science and Technology through theSOWACOR project.
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorMeulepas, Roel J.W.en
dc.contributor.authorJagersma, Christian G.en
dc.contributor.authorZhang, Yuen
dc.contributor.authorPetrillo, Micheleen
dc.contributor.authorCai, Hengzheen
dc.contributor.authorBuisman, Cees J.N.en
dc.contributor.authorStams, Alfons J.M.en
dc.contributor.authorLens, Piet N.L.en
dc.date.accessioned2016-02-28T06:35:07Zen
dc.date.available2016-02-28T06:35:07Zen
dc.date.issued2010-05en
dc.identifier.citationMeulepas RJW, Jagersma CG, Zhang Y, Petrillo M, Cai H, et al. (2010) Trace methane oxidation and the methane dependency of sulfate reduction in anaerobic granular sludge. FEMS Microbiology Ecology 72: 261–271. Available: http://dx.doi.org/10.1111/j.1574-6941.2010.00849.x.en
dc.identifier.issn0168-6496en
dc.identifier.issn1574-6941en
dc.identifier.pmid20337708en
dc.identifier.doi10.1111/j.1574-6941.2010.00849.xen
dc.identifier.urihttp://hdl.handle.net/10754/600053en
dc.description.abstractThis study investigates the oxidation of labeled methane (CH4) and the CH4 dependence of sulfate reduction in three types of anaerobic granular sludge. In all samples, 13C-labeled CH4 was anaerobically oxidized to 13C-labeled CO2, while net endogenous CH4 production was observed. Labeled-CH4 oxidation rates followed CH4 production rates, and the presence of sulfate hampered both labeled-CH4 oxidation and methanogenesis. Labeled-CH4 oxidation was therefore linked to methanogenesis. This process is referred to as trace CH4 oxidation and has been demonstrated in methanogenic pure cultures. This study shows that the ratio between labeled-CH4 oxidation and methanogenesis is positively affected by the CH4 partial pressure and that this ratio is in methanogenic granular sludge more than 40 times higher than that in pure cultures of methanogens. The CH4 partial pressure also positively affected sulfate reduction and negatively affected methanogenesis: a repression of methanogenesis at elevated CH4 partial pressures confers an advantage to sulfate reducers that compete with methanogens for common substrates, formed from endogenous material. The oxidation of labeled CH 4 and the CH4 dependence of sulfate reduction are thus not necessarily evidence of anaerobic oxidation of CH4 coupled to sulfate reduction. © 2010 Federation of European Microbiological Societies.en
dc.description.sponsorshipThis work was part of the Anaerobic Methane Oxidation forSulfate Reduction project (AMethOx for SuRe, numberEETK03044) supported by the Dutch ministries of Economicalaffairs, Education, culture and science and Environmentand special planning as part their EET (Economie, Ecologie,Technologie) program. The research was cofunded by KingAbdullah University of Science and Technology through theSOWACOR project.en
dc.publisherOxford University Press (OUP)en
dc.subjectAnaerobic granular sludgeen
dc.subjectReversed methanogenesisen
dc.subjectSulfate reductionen
dc.subjectTrace methane oxidationen
dc.titleTrace methane oxidation and the methane dependency of sulfate reduction in anaerobic granular sludgeen
dc.typeArticleen
dc.identifier.journalFEMS Microbiology Ecologyen
dc.contributor.institutionWageningen University and Research Centre, Wageningen, Netherlandsen
dc.contributor.institutionUNESCO-IHE Institute for Water Education, Delft, Netherlandsen

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