Three-Dimensional Protein Fold Determination from Backbone Amide Pseudocontact Shifts Generated by Lanthanide Tags at Multiple Sites

Handle URI:
http://hdl.handle.net/10754/600019
Title:
Three-Dimensional Protein Fold Determination from Backbone Amide Pseudocontact Shifts Generated by Lanthanide Tags at Multiple Sites
Authors:
Yagi, Hiromasa; Pilla, Kala Bharath; Maleckis, Ansis; Graham, Bim; Huber, Thomas; Otting, Gottfried
Abstract:
Site-specific attachment of paramagnetic lanthanide ions to a protein generates pseudocontact shifts (PCS) in the nuclear magnetic resonance (NMR) spectra of the protein that are easily measured as changes in chemical shifts. By labeling the protein with lanthanide tags at four different sites, PCSs are observed for most amide protons and accurate information is obtained about their coordinates in three-dimensional space. The approach is demonstrated with the chaperone ERp29, for which large differences have been reported between X-ray and NMR structures of the C-terminal domain, ERp29-C. The results unambiguously show that the structure of rat ERp29-C in solution is similar to the crystal structure of human ERp29-C. PCSs of backbone amides were the only structural restraints required. Because these can be measured for more dilute protein solutions than other NMR restraints, the approach greatly widens the range of proteins amenable to structural studies in solution. © 2013 Elsevier Ltd. All rights reserved.
Citation:
Yagi H, Pilla KB, Maleckis A, Graham B, Huber T, et al. (2013) Three-Dimensional Protein Fold Determination from Backbone Amide Pseudocontact Shifts Generated by Lanthanide Tags at Multiple Sites. Structure 21: 883–890. Available: http://dx.doi.org/10.1016/j.str.2013.04.001.
Publisher:
Elsevier BV
Journal:
Structure
Issue Date:
Jun-2013
DOI:
10.1016/j.str.2013.04.001
PubMed ID:
23643949
Type:
Article
ISSN:
0969-2126
Sponsors:
We thank Dr. Souren Mkrtchian for the plasmid encoding ERp29 and the supercomputing facility at the King Abdulla University of Science and Technology (KAUST, Saudi Arabia) for providing access to the Blue Gene/P (Shaheen) supercomputer. Financial support by the Australian Research Council, including a Future Fellowship to T.H., is gratefully acknowledged.
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Full metadata record

DC FieldValue Language
dc.contributor.authorYagi, Hiromasaen
dc.contributor.authorPilla, Kala Bharathen
dc.contributor.authorMaleckis, Ansisen
dc.contributor.authorGraham, Bimen
dc.contributor.authorHuber, Thomasen
dc.contributor.authorOtting, Gottfrieden
dc.date.accessioned2016-02-28T06:34:28Zen
dc.date.available2016-02-28T06:34:28Zen
dc.date.issued2013-06en
dc.identifier.citationYagi H, Pilla KB, Maleckis A, Graham B, Huber T, et al. (2013) Three-Dimensional Protein Fold Determination from Backbone Amide Pseudocontact Shifts Generated by Lanthanide Tags at Multiple Sites. Structure 21: 883–890. Available: http://dx.doi.org/10.1016/j.str.2013.04.001.en
dc.identifier.issn0969-2126en
dc.identifier.pmid23643949en
dc.identifier.doi10.1016/j.str.2013.04.001en
dc.identifier.urihttp://hdl.handle.net/10754/600019en
dc.description.abstractSite-specific attachment of paramagnetic lanthanide ions to a protein generates pseudocontact shifts (PCS) in the nuclear magnetic resonance (NMR) spectra of the protein that are easily measured as changes in chemical shifts. By labeling the protein with lanthanide tags at four different sites, PCSs are observed for most amide protons and accurate information is obtained about their coordinates in three-dimensional space. The approach is demonstrated with the chaperone ERp29, for which large differences have been reported between X-ray and NMR structures of the C-terminal domain, ERp29-C. The results unambiguously show that the structure of rat ERp29-C in solution is similar to the crystal structure of human ERp29-C. PCSs of backbone amides were the only structural restraints required. Because these can be measured for more dilute protein solutions than other NMR restraints, the approach greatly widens the range of proteins amenable to structural studies in solution. © 2013 Elsevier Ltd. All rights reserved.en
dc.description.sponsorshipWe thank Dr. Souren Mkrtchian for the plasmid encoding ERp29 and the supercomputing facility at the King Abdulla University of Science and Technology (KAUST, Saudi Arabia) for providing access to the Blue Gene/P (Shaheen) supercomputer. Financial support by the Australian Research Council, including a Future Fellowship to T.H., is gratefully acknowledged.en
dc.publisherElsevier BVen
dc.titleThree-Dimensional Protein Fold Determination from Backbone Amide Pseudocontact Shifts Generated by Lanthanide Tags at Multiple Sitesen
dc.typeArticleen
dc.identifier.journalStructureen
dc.contributor.institutionAustralian National University, Canberra, Australiaen
dc.contributor.institutionMonash Institute of Pharmaceutical Sciences, Melbourne, Australiaen
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