The Influence of Bioreactor Geometry and the Mechanical Environment on Engineered Tissues

Handle URI:
http://hdl.handle.net/10754/599920
Title:
The Influence of Bioreactor Geometry and the Mechanical Environment on Engineered Tissues
Authors:
Osborne, J. M.; O’Dea, R. D.; Whiteley, J. P.; Byrne, H. M.; Waters, S. L.
Abstract:
A three phase model for the growth of a tissue construct within a perfusion bioreactor is examined. The cell population (and attendant extracellular matrix), culture medium, and porous scaffold are treated as distinct phases. The bioreactor system is represented by a two-dimensional channel containing a cell-seeded rigid porous scaffold (tissue construct), which is perfused with a culture medium. Through the prescription of appropriate functional forms for cell proliferation and extracellular matrix deposition rates, the model is used to compare the influence of cell density-, pressure-, and culture medium shear stress-regulated growth on the composition of the engineered tissue. The governing equations are derived in O'Dea et al. "A Three Phase Model for Tissue Construct Growth in a Perfusion Bioreactor," Math. Med. Biol., in which the long-wavelength limit was exploited to aid analysis; here, finite element methods are used to construct two-dimensional solutions to the governing equations and to investigate thoroughly their behavior. Comparison of the total tissue yield and averaged pressures, velocities, and shear stress demonstrates that quantitative agreement between the two-dimensional and long-wavelength approximation solutions is obtained for channel aspect ratios of order 10 -2 and that much of the qualitative behavior of the model is captured in the long-wavelength limit, even for relatively large channel aspect ratios. However, we demonstrate that in order to capture accurately the effect of mechanotransduction mechanisms on tissue construct growth, spatial effects in at least two dimensions must be included due to the inherent spatial variation of mechanical stimuli relevant to perfusion bioreactors, most notably, fluid shear stress, a feature not captured in the long-wavelength limit. Copyright © 2010 by ASME.
Citation:
Osborne JM, O’Dea RD, Whiteley JP, Byrne HM, Waters SL (2010) The Influence of Bioreactor Geometry and the Mechanical Environment on Engineered Tissues. J Biomech Eng 132: 051006. Available: http://dx.doi.org/10.1115/1.4001160.
Publisher:
ASME International
Journal:
Journal of Biomechanical Engineering
KAUST Grant Number:
KUK-C1-013-04
Issue Date:
2010
DOI:
10.1115/1.4001160
PubMed ID:
20459207
Type:
Article
ISSN:
0148-0731
Sponsors:
J.M.O. is supported by the EPSRC/BBSRC funded OCISB Project No. BB/D020190/1, and much of this work was undertaken while funded under a LSI DTC studentship. R.D.O. is supported by an EPSRC Ph.D. studentship. J.P.W. is supported by Award No. KUK-C1-013-04, made by King Abdullah University of Science and Technology (KAUST). S.L.W. gratefully acknowledges funding from the EPSRC in the form of an Advanced Research Fellowship.
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorOsborne, J. M.en
dc.contributor.authorO’Dea, R. D.en
dc.contributor.authorWhiteley, J. P.en
dc.contributor.authorByrne, H. M.en
dc.contributor.authorWaters, S. L.en
dc.date.accessioned2016-02-28T06:32:28Zen
dc.date.available2016-02-28T06:32:28Zen
dc.date.issued2010en
dc.identifier.citationOsborne JM, O’Dea RD, Whiteley JP, Byrne HM, Waters SL (2010) The Influence of Bioreactor Geometry and the Mechanical Environment on Engineered Tissues. J Biomech Eng 132: 051006. Available: http://dx.doi.org/10.1115/1.4001160.en
dc.identifier.issn0148-0731en
dc.identifier.pmid20459207en
dc.identifier.doi10.1115/1.4001160en
dc.identifier.urihttp://hdl.handle.net/10754/599920en
dc.description.abstractA three phase model for the growth of a tissue construct within a perfusion bioreactor is examined. The cell population (and attendant extracellular matrix), culture medium, and porous scaffold are treated as distinct phases. The bioreactor system is represented by a two-dimensional channel containing a cell-seeded rigid porous scaffold (tissue construct), which is perfused with a culture medium. Through the prescription of appropriate functional forms for cell proliferation and extracellular matrix deposition rates, the model is used to compare the influence of cell density-, pressure-, and culture medium shear stress-regulated growth on the composition of the engineered tissue. The governing equations are derived in O'Dea et al. "A Three Phase Model for Tissue Construct Growth in a Perfusion Bioreactor," Math. Med. Biol., in which the long-wavelength limit was exploited to aid analysis; here, finite element methods are used to construct two-dimensional solutions to the governing equations and to investigate thoroughly their behavior. Comparison of the total tissue yield and averaged pressures, velocities, and shear stress demonstrates that quantitative agreement between the two-dimensional and long-wavelength approximation solutions is obtained for channel aspect ratios of order 10 -2 and that much of the qualitative behavior of the model is captured in the long-wavelength limit, even for relatively large channel aspect ratios. However, we demonstrate that in order to capture accurately the effect of mechanotransduction mechanisms on tissue construct growth, spatial effects in at least two dimensions must be included due to the inherent spatial variation of mechanical stimuli relevant to perfusion bioreactors, most notably, fluid shear stress, a feature not captured in the long-wavelength limit. Copyright © 2010 by ASME.en
dc.description.sponsorshipJ.M.O. is supported by the EPSRC/BBSRC funded OCISB Project No. BB/D020190/1, and much of this work was undertaken while funded under a LSI DTC studentship. R.D.O. is supported by an EPSRC Ph.D. studentship. J.P.W. is supported by Award No. KUK-C1-013-04, made by King Abdullah University of Science and Technology (KAUST). S.L.W. gratefully acknowledges funding from the EPSRC in the form of an Advanced Research Fellowship.en
dc.publisherASME Internationalen
dc.titleThe Influence of Bioreactor Geometry and the Mechanical Environment on Engineered Tissuesen
dc.typeArticleen
dc.identifier.journalJournal of Biomechanical Engineeringen
dc.contributor.institutionUniversity of Oxford, Oxford, United Kingdomen
dc.contributor.institutionUniversity of Nottingham, Nottingham, United Kingdomen
kaust.grant.numberKUK-C1-013-04en
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