Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol

Handle URI:
http://hdl.handle.net/10754/599776
Title:
Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol
Authors:
Liu, Xiang; Bastian, Sabine; Snow, Christopher D.; Brustad, Eric M.; Saleski, Tatyana E.; Xu, Jian-He; Meinhold, Peter; Arnold, Frances H.
Abstract:
We have determined the X-ray crystal structures of the NADH-dependent alcohol dehydrogenase LlAdhA from Lactococcus lactis and its laboratory-evolved variant LlAdhA(RE1) at 1.9Å and 2.5Å resolution, respectively. LlAdhA(RE1), which contains three amino acid mutations (Y50F, I212T, and L264V), was engineered to increase the microbial production of isobutanol (2-methylpropan-1-ol) from isobutyraldehyde (2-methylpropanal). Structural comparison of LlAdhA and LlAdhA(RE1) indicates that the enhanced activity on isobutyraldehyde stems from increases in the protein's active site size, hydrophobicity, and substrate access. Further structure-guided mutagenesis generated a quadruple mutant (Y50F/N110S/I212T/L264V), whose KM for isobutyraldehyde is ∼17-fold lower and catalytic efficiency (kcat/KM) is ∼160-fold higher than wild-type LlAdhA. Combining detailed structural information and directed evolution, we have achieved significant improvements in non-native alcohol dehydrogenase activity that will facilitate the production of next-generation fuels such as isobutanol from renewable resources.
Citation:
Liu X, Bastian S, Snow CD, Brustad EM, Saleski TE, et al. (2013) Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol. Journal of Biotechnology 164: 188–195. Available: http://dx.doi.org/10.1016/j.jbiotec.2012.08.008.
Publisher:
Elsevier BV
Journal:
Journal of Biotechnology
KAUST Grant Number:
KUS-F1-028-03
Issue Date:
Mar-2013
DOI:
10.1016/j.jbiotec.2012.08.008
PubMed ID:
22974724
PubMed Central ID:
PMC3542407
Type:
Article
ISSN:
0168-1656
Sponsors:
This research was sponsored by the Army Research Laboratory and was accomplished under Cooperative Agreement Number W911NF-09-2-0022. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Laboratory or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. X.L. received support from the China Scholarship Council (CSC), E.M.B. was supported by a Ruth L Kirschstein National Research Service Award (1F32-GM087102) from the National Institutes of Health, and C.D.S. was supported by a research fellowship (KUS-F1-028-03) from King Abdullah University of Science and Technology (KAUST). The Molecular Observatory is supported by the Gordon and Betty Moore Foundation, the Beckman Institute and the Sanofi-Aventis Bioengineering Research Program at Caltech.
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Full metadata record

DC FieldValue Language
dc.contributor.authorLiu, Xiangen
dc.contributor.authorBastian, Sabineen
dc.contributor.authorSnow, Christopher D.en
dc.contributor.authorBrustad, Eric M.en
dc.contributor.authorSaleski, Tatyana E.en
dc.contributor.authorXu, Jian-Heen
dc.contributor.authorMeinhold, Peteren
dc.contributor.authorArnold, Frances H.en
dc.date.accessioned2016-02-28T06:09:33Zen
dc.date.available2016-02-28T06:09:33Zen
dc.date.issued2013-03en
dc.identifier.citationLiu X, Bastian S, Snow CD, Brustad EM, Saleski TE, et al. (2013) Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol. Journal of Biotechnology 164: 188–195. Available: http://dx.doi.org/10.1016/j.jbiotec.2012.08.008.en
dc.identifier.issn0168-1656en
dc.identifier.pmid22974724en
dc.identifier.doi10.1016/j.jbiotec.2012.08.008en
dc.identifier.urihttp://hdl.handle.net/10754/599776en
dc.description.abstractWe have determined the X-ray crystal structures of the NADH-dependent alcohol dehydrogenase LlAdhA from Lactococcus lactis and its laboratory-evolved variant LlAdhA(RE1) at 1.9Å and 2.5Å resolution, respectively. LlAdhA(RE1), which contains three amino acid mutations (Y50F, I212T, and L264V), was engineered to increase the microbial production of isobutanol (2-methylpropan-1-ol) from isobutyraldehyde (2-methylpropanal). Structural comparison of LlAdhA and LlAdhA(RE1) indicates that the enhanced activity on isobutyraldehyde stems from increases in the protein's active site size, hydrophobicity, and substrate access. Further structure-guided mutagenesis generated a quadruple mutant (Y50F/N110S/I212T/L264V), whose KM for isobutyraldehyde is ∼17-fold lower and catalytic efficiency (kcat/KM) is ∼160-fold higher than wild-type LlAdhA. Combining detailed structural information and directed evolution, we have achieved significant improvements in non-native alcohol dehydrogenase activity that will facilitate the production of next-generation fuels such as isobutanol from renewable resources.en
dc.description.sponsorshipThis research was sponsored by the Army Research Laboratory and was accomplished under Cooperative Agreement Number W911NF-09-2-0022. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Laboratory or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. X.L. received support from the China Scholarship Council (CSC), E.M.B. was supported by a Ruth L Kirschstein National Research Service Award (1F32-GM087102) from the National Institutes of Health, and C.D.S. was supported by a research fellowship (KUS-F1-028-03) from King Abdullah University of Science and Technology (KAUST). The Molecular Observatory is supported by the Gordon and Betty Moore Foundation, the Beckman Institute and the Sanofi-Aventis Bioengineering Research Program at Caltech.en
dc.publisherElsevier BVen
dc.subjectAlcohol dehydrogenaseen
dc.subjectBiofuelen
dc.subjectCrystal structureen
dc.subjectDirected evolutionen
dc.subjectIsobutyraldehydeen
dc.subjectSite-saturation mutagenesisen
dc.titleStructure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanolen
dc.typeArticleen
dc.identifier.journalJournal of Biotechnologyen
dc.identifier.pmcidPMC3542407en
dc.contributor.institutionDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.en
kaust.grant.numberKUS-F1-028-03en

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