siRNA transfection in larvae of the barnacle Amphibalanus amphitrite

Handle URI:
http://hdl.handle.net/10754/599643
Title:
siRNA transfection in larvae of the barnacle Amphibalanus amphitrite
Authors:
Zhang, G.; He, L.-S.; Wong, Y. H.; Yu, L.; Qian, P.-Y.
Abstract:
RNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in ‘double transfections’, in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. A relatively longer transfection time and more larval cells directly exposed to siRNA might explain the higher efficiency of double transfection experiments.
Citation:
Zhang G, He L-S, Wong YH, Yu L, Qian P-Y (2015) siRNA transfection in larvae of the barnacle Amphibalanus amphitrite. Journal of Experimental Biology 218: 2505–2509. Available: http://dx.doi.org/10.1242/jeb.120113.
Publisher:
The Company of Biologists
Journal:
Journal of Experimental Biology
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
25-Jun-2015
DOI:
10.1242/jeb.120113
PubMed ID:
26113139
Type:
Article
ISSN:
0022-0949; 1477-9145
Sponsors:
This work was supported by grants from China Ocean Mineral Resources Research and Development Association (DY125-15-T-02), the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and the Research Grants Council of the Hong Kong Special Administrative Region (GRF661611 and GRF662413) to P.-Y.Q. as well as the National Natural Science Foundation of China (31460092) to L.-S.H.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, G.en
dc.contributor.authorHe, L.-S.en
dc.contributor.authorWong, Y. H.en
dc.contributor.authorYu, L.en
dc.contributor.authorQian, P.-Y.en
dc.date.accessioned2016-02-28T06:06:32Zen
dc.date.available2016-02-28T06:06:32Zen
dc.date.issued2015-06-25en
dc.identifier.citationZhang G, He L-S, Wong YH, Yu L, Qian P-Y (2015) siRNA transfection in larvae of the barnacle Amphibalanus amphitrite. Journal of Experimental Biology 218: 2505–2509. Available: http://dx.doi.org/10.1242/jeb.120113.en
dc.identifier.issn0022-0949en
dc.identifier.issn1477-9145en
dc.identifier.pmid26113139en
dc.identifier.doi10.1242/jeb.120113en
dc.identifier.urihttp://hdl.handle.net/10754/599643en
dc.description.abstractRNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in ‘double transfections’, in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. A relatively longer transfection time and more larval cells directly exposed to siRNA might explain the higher efficiency of double transfection experiments.en
dc.description.sponsorshipThis work was supported by grants from China Ocean Mineral Resources Research and Development Association (DY125-15-T-02), the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and the Research Grants Council of the Hong Kong Special Administrative Region (GRF661611 and GRF662413) to P.-Y.Q. as well as the National Natural Science Foundation of China (31460092) to L.-S.H.en
dc.publisherThe Company of Biologistsen
dc.titlesiRNA transfection in larvae of the barnacle Amphibalanus amphitriteen
dc.typeArticleen
dc.identifier.journalJournal of Experimental Biologyen
dc.contributor.institutionEnvironmental Science Programs, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, Chinaen
dc.contributor.institutionKAUST Global Collaborative Research Program, Division of Life Science, School of Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, Chinaen
dc.contributor.institutionSanya Institute of Deep-sea Science and Engineering,Chinese AcademyofScience, No. 62,Fenghuang Road, SanyaCity, Hainan Province 572000, China.en
kaust.authorZhang, G.en
kaust.authorHe, L.-S.en
kaust.authorWong, Y. H.en
kaust.authorYu, L.en
kaust.authorQian, P.-Y.en
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en

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