Reference and counter electrode positions affect electrochemical characterization of bioanodes in different bioelectrochemical systems

Handle URI:
http://hdl.handle.net/10754/599480
Title:
Reference and counter electrode positions affect electrochemical characterization of bioanodes in different bioelectrochemical systems
Authors:
Zhang, Fang; Liu, Jia; Ivanov, Ivan; Hatzell, Marta C.; Yang, Wulin; Ahn, Yongtae; Logan, Bruce E.
Abstract:
The placement of the reference electrode (RE) in various bioelectrochemical systems is often varied to accommodate different reactor configurations. While the effect of the RE placement is well understood from a strictly electrochemistry perspective, there are impacts on exoelectrogenic biofilms in engineered systems that have not been adequately addressed. Varying distances between the working electrode (WE) and the RE, or the RE and the counter electrode (CE) in microbial fuel cells (MFCs) can alter bioanode characteristics. With well-spaced anode and cathode distances in an MFC, increasing the distance between the RE and anode (WE) altered bioanode cyclic voltammograms (CVs) due to the uncompensated ohmic drop. Electrochemical impedance spectra (EIS) also changed with RE distances, resulting in a calculated increase in anode resistance that varied between 17 and 31Ω (-0.2V). While WE potentials could be corrected with ohmic drop compensation during the CV tests, they could not be automatically corrected by the potentiostat in the EIS tests. The electrochemical characteristics of bioanodes were altered by their acclimation to different anode potentials that resulted from varying the distance between the RE and the CE (cathode). These differences were true changes in biofilm characteristics because the CVs were electrochemically independent of conditions resulting from changing CE to RE distances. Placing the RE outside of the current path enabled accurate bioanode characterization using CVs and EIS due to negligible ohmic resistances (0.4Ω). It is therefore concluded for bioelectrochemical systems that when possible, the RE should be placed outside the current path and near the WE, as this will result in more accurate representation of bioanode characteristics. © 2014 Wiley Periodicals, Inc.
Citation:
Zhang F, Liu J, Ivanov I, Hatzell MC, Yang W, et al. (2014) Reference and counter electrode positions affect electrochemical characterization of bioanodes in different bioelectrochemical systems. Biotechnology and Bioengineering 111: 1931–1939. Available: http://dx.doi.org/10.1002/bit.25253.
Publisher:
Wiley-Blackwell
Journal:
Biotechnology and Bioengineering
KAUST Grant Number:
KUS-I1-003-13
Issue Date:
16-Jun-2014
DOI:
10.1002/bit.25253
PubMed ID:
24729040
Type:
Article
ISSN:
0006-3592
Sponsors:
Contract grant sponsor: Award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST)
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Fangen
dc.contributor.authorLiu, Jiaen
dc.contributor.authorIvanov, Ivanen
dc.contributor.authorHatzell, Marta C.en
dc.contributor.authorYang, Wulinen
dc.contributor.authorAhn, Yongtaeen
dc.contributor.authorLogan, Bruce E.en
dc.date.accessioned2016-02-28T05:51:54Zen
dc.date.available2016-02-28T05:51:54Zen
dc.date.issued2014-06-16en
dc.identifier.citationZhang F, Liu J, Ivanov I, Hatzell MC, Yang W, et al. (2014) Reference and counter electrode positions affect electrochemical characterization of bioanodes in different bioelectrochemical systems. Biotechnology and Bioengineering 111: 1931–1939. Available: http://dx.doi.org/10.1002/bit.25253.en
dc.identifier.issn0006-3592en
dc.identifier.pmid24729040en
dc.identifier.doi10.1002/bit.25253en
dc.identifier.urihttp://hdl.handle.net/10754/599480en
dc.description.abstractThe placement of the reference electrode (RE) in various bioelectrochemical systems is often varied to accommodate different reactor configurations. While the effect of the RE placement is well understood from a strictly electrochemistry perspective, there are impacts on exoelectrogenic biofilms in engineered systems that have not been adequately addressed. Varying distances between the working electrode (WE) and the RE, or the RE and the counter electrode (CE) in microbial fuel cells (MFCs) can alter bioanode characteristics. With well-spaced anode and cathode distances in an MFC, increasing the distance between the RE and anode (WE) altered bioanode cyclic voltammograms (CVs) due to the uncompensated ohmic drop. Electrochemical impedance spectra (EIS) also changed with RE distances, resulting in a calculated increase in anode resistance that varied between 17 and 31Ω (-0.2V). While WE potentials could be corrected with ohmic drop compensation during the CV tests, they could not be automatically corrected by the potentiostat in the EIS tests. The electrochemical characteristics of bioanodes were altered by their acclimation to different anode potentials that resulted from varying the distance between the RE and the CE (cathode). These differences were true changes in biofilm characteristics because the CVs were electrochemically independent of conditions resulting from changing CE to RE distances. Placing the RE outside of the current path enabled accurate bioanode characterization using CVs and EIS due to negligible ohmic resistances (0.4Ω). It is therefore concluded for bioelectrochemical systems that when possible, the RE should be placed outside the current path and near the WE, as this will result in more accurate representation of bioanode characteristics. © 2014 Wiley Periodicals, Inc.en
dc.description.sponsorshipContract grant sponsor: Award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST)en
dc.publisherWiley-Blackwellen
dc.subjectBioanodeen
dc.subjectBioelectrochemical systemsen
dc.subjectCyclic voltammetryen
dc.subjectElectrochemical impedance spectroscopyen
dc.subjectReference electrode placementen
dc.subjectUncompensated ohmic dropen
dc.titleReference and counter electrode positions affect electrochemical characterization of bioanodes in different bioelectrochemical systemsen
dc.typeArticleen
dc.identifier.journalBiotechnology and Bioengineeringen
dc.contributor.institutionDepartment of Civil and Environmental Engineering; Penn State University; 212 Sackett Building University Park Pennsylvania 16802en
dc.contributor.institutionDepartment of Energy Engineering; Gyeongnam National University of Science and Technology; Dongjin-ro 33 Jinju Gyeongnam 660-758 Koreaen
kaust.grant.numberKUS-I1-003-13en

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