Quantifying the Traction Force of a Single Cell by Aligned Silicon Nanowire Array

Handle URI:
http://hdl.handle.net/10754/599423
Title:
Quantifying the Traction Force of a Single Cell by Aligned Silicon Nanowire Array
Authors:
Li, Zhou; Song, Jinhui; Mantini, Giulia; Lu, Ming-Yen; Fang, Hao; Falconi, Christian; Chen, Lih-Juann; Wang, Zhong Lin
Abstract:
The physical behaviors of stationary cells, such as the morphology, motility, adhesion, anchorage, invasion and metastasis, are likely to be important for governing their biological characteristics. A change in the physical properties of mammalian cells could be an indication of disease. In this paper, we present a silicon-nanowire-array based technique for quantifying the mechanical behavior of single cells representing three distinct groups: normal mammalian cells, benign cells (L929), and malignant cells (HeLa). By culturing the cells on top of NW arrays, the maximum traction forces of two different tumor cells (HeLa, L929) have been measured by quantitatively analyzing the bending of the nanowires. The cancer cell exhibits a larger traction force than the normal cell by ∼20% for a HeLa cell and ∼50% for a L929 cell. The traction forces have been measured for the L929 cells and mechanocytes as a function of culture time. The relationship between cells extending area and their traction force has been investigated. Our study is likely important for studying the mechanical properties of single cells and their migration characteristics, possibly providing a new cellular level diagnostic technique. © 2009 American Chemical Society.
Citation:
Li Z, Song J, Mantini G, Lu M-Y, Fang H, et al. (2009) Quantifying the Traction Force of a Single Cell by Aligned Silicon Nanowire Array. Nano Lett 9: 3575–3580. Available: http://dx.doi.org/10.1021/nl901774m.
Publisher:
American Chemical Society (ACS)
Journal:
Nano Letters
Issue Date:
14-Oct-2009
DOI:
10.1021/nl901774m
PubMed ID:
19824706
Type:
Article
ISSN:
1530-6984; 1530-6992
Sponsors:
Research supported by DARPA (Army/AMCOM/REDSTONE AR, W31P4Q-08-1-0009), BES DOE (DE-FG02-07ER46394), KAUST Global Research Partnership, Emory-Georgia Tech CCNE from NIH, and NSF (DMS 0706436, CMMI 0403671). Z.L. and H.F. are joint students between Georgia Tech and Peking University, and they thank the partial fellowship support by the China Scholarship Council (CSC) (No. 20073020) that supported them to carry out the research at Georgia Tech. M.-Y.L. was on a ROC National Science Council Fellowship for conducting research at Georgia Tech.
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Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Zhouen
dc.contributor.authorSong, Jinhuien
dc.contributor.authorMantini, Giuliaen
dc.contributor.authorLu, Ming-Yenen
dc.contributor.authorFang, Haoen
dc.contributor.authorFalconi, Christianen
dc.contributor.authorChen, Lih-Juannen
dc.contributor.authorWang, Zhong Linen
dc.date.accessioned2016-02-28T05:50:51Zen
dc.date.available2016-02-28T05:50:51Zen
dc.date.issued2009-10-14en
dc.identifier.citationLi Z, Song J, Mantini G, Lu M-Y, Fang H, et al. (2009) Quantifying the Traction Force of a Single Cell by Aligned Silicon Nanowire Array. Nano Lett 9: 3575–3580. Available: http://dx.doi.org/10.1021/nl901774m.en
dc.identifier.issn1530-6984en
dc.identifier.issn1530-6992en
dc.identifier.pmid19824706en
dc.identifier.doi10.1021/nl901774men
dc.identifier.urihttp://hdl.handle.net/10754/599423en
dc.description.abstractThe physical behaviors of stationary cells, such as the morphology, motility, adhesion, anchorage, invasion and metastasis, are likely to be important for governing their biological characteristics. A change in the physical properties of mammalian cells could be an indication of disease. In this paper, we present a silicon-nanowire-array based technique for quantifying the mechanical behavior of single cells representing three distinct groups: normal mammalian cells, benign cells (L929), and malignant cells (HeLa). By culturing the cells on top of NW arrays, the maximum traction forces of two different tumor cells (HeLa, L929) have been measured by quantitatively analyzing the bending of the nanowires. The cancer cell exhibits a larger traction force than the normal cell by ∼20% for a HeLa cell and ∼50% for a L929 cell. The traction forces have been measured for the L929 cells and mechanocytes as a function of culture time. The relationship between cells extending area and their traction force has been investigated. Our study is likely important for studying the mechanical properties of single cells and their migration characteristics, possibly providing a new cellular level diagnostic technique. © 2009 American Chemical Society.en
dc.description.sponsorshipResearch supported by DARPA (Army/AMCOM/REDSTONE AR, W31P4Q-08-1-0009), BES DOE (DE-FG02-07ER46394), KAUST Global Research Partnership, Emory-Georgia Tech CCNE from NIH, and NSF (DMS 0706436, CMMI 0403671). Z.L. and H.F. are joint students between Georgia Tech and Peking University, and they thank the partial fellowship support by the China Scholarship Council (CSC) (No. 20073020) that supported them to carry out the research at Georgia Tech. M.-Y.L. was on a ROC National Science Council Fellowship for conducting research at Georgia Tech.en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleQuantifying the Traction Force of a Single Cell by Aligned Silicon Nanowire Arrayen
dc.typeArticleen
dc.identifier.journalNano Lettersen
dc.contributor.institutionPeking University, Beijing, Chinaen
dc.contributor.institutionGeorgia Institute of Technology, Atlanta, United Statesen
dc.contributor.institutionUniversita degli Studi di Roma Tor Vergata, Rome, Italyen
dc.contributor.institutionNational Tsing Hua University, Hsin-chu, Taiwanen

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