Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface

Handle URI:
http://hdl.handle.net/10754/599364
Title:
Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface
Authors:
Capriotti, Anna Laura; Caracciolo, Giulio; Cavaliere, Chiara; Crescenzi, Carlo; Pozzi, Daniela; Laganà, Aldo
Abstract:
The knowledge about the interaction between plasma proteins and nanocarriers employed for in vivo delivery is fundamental to understand their biodistribution. Protein adsorption onto nanoparticle surface (protein corona) is strongly affected by vector surface characteristics. In general, the primary interaction is thought to be electrostatic, thus surface charge of carrier is supposed to play a central role in protein adsorption. Because protein corona composition can be critical in modifying the interactive surface that is recognized by cells, characterizing its formation onto lipid particles may serve as a fundamental predictive model for the in vivo efficiency of a lipidic vector. In the present work, protein coronas adsorbed onto three differently charged cationic liposome formulations were compared by a shotgun proteomic approach based on nano-liquid chromatography-high-resolution mass spectrometry. About 130 proteins were identified in each corona, with only small differences between the different cationic liposome formulations. However, this study could be useful for the future controlled design of colloidal drug carriers and possibly in the controlled creation of biocompatible surfaces of other devices that come into contact with proteins into body fluids. © 2011 Springer-Verlag.
Citation:
Capriotti AL, Caracciolo G, Cavaliere C, Crescenzi C, Pozzi D, et al. (2011) Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface. Analytical and Bioanalytical Chemistry 401: 1195–1202. Available: http://dx.doi.org/10.1007/s00216-011-5188-8.
Publisher:
Springer Nature
Journal:
Analytical and Bioanalytical Chemistry
KAUST Grant Number:
KUK-F1-036-32
Issue Date:
2-Jul-2011
DOI:
10.1007/s00216-011-5188-8
PubMed ID:
21725631
Type:
Article
ISSN:
1618-2642; 1618-2650
Sponsors:
This publication is based on work supported by Award No. KUK-F1-036-32, made by King Abdullah University of Science and Technology (KAUST).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorCapriotti, Anna Lauraen
dc.contributor.authorCaracciolo, Giulioen
dc.contributor.authorCavaliere, Chiaraen
dc.contributor.authorCrescenzi, Carloen
dc.contributor.authorPozzi, Danielaen
dc.contributor.authorLaganà, Aldoen
dc.date.accessioned2016-02-28T06:05:38Zen
dc.date.available2016-02-28T06:05:38Zen
dc.date.issued2011-07-02en
dc.identifier.citationCapriotti AL, Caracciolo G, Cavaliere C, Crescenzi C, Pozzi D, et al. (2011) Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface. Analytical and Bioanalytical Chemistry 401: 1195–1202. Available: http://dx.doi.org/10.1007/s00216-011-5188-8.en
dc.identifier.issn1618-2642en
dc.identifier.issn1618-2650en
dc.identifier.pmid21725631en
dc.identifier.doi10.1007/s00216-011-5188-8en
dc.identifier.urihttp://hdl.handle.net/10754/599364en
dc.description.abstractThe knowledge about the interaction between plasma proteins and nanocarriers employed for in vivo delivery is fundamental to understand their biodistribution. Protein adsorption onto nanoparticle surface (protein corona) is strongly affected by vector surface characteristics. In general, the primary interaction is thought to be electrostatic, thus surface charge of carrier is supposed to play a central role in protein adsorption. Because protein corona composition can be critical in modifying the interactive surface that is recognized by cells, characterizing its formation onto lipid particles may serve as a fundamental predictive model for the in vivo efficiency of a lipidic vector. In the present work, protein coronas adsorbed onto three differently charged cationic liposome formulations were compared by a shotgun proteomic approach based on nano-liquid chromatography-high-resolution mass spectrometry. About 130 proteins were identified in each corona, with only small differences between the different cationic liposome formulations. However, this study could be useful for the future controlled design of colloidal drug carriers and possibly in the controlled creation of biocompatible surfaces of other devices that come into contact with proteins into body fluids. © 2011 Springer-Verlag.en
dc.description.sponsorshipThis publication is based on work supported by Award No. KUK-F1-036-32, made by King Abdullah University of Science and Technology (KAUST).en
dc.publisherSpringer Natureen
dc.subjectCationic liposomesen
dc.subjectMass spectrometryen
dc.subjectNanoparticlesen
dc.subjectPlasmaen
dc.subjectProtein coronaen
dc.titleShotgun proteomic analytical approach for studying proteins adsorbed onto liposome surfaceen
dc.typeArticleen
dc.identifier.journalAnalytical and Bioanalytical Chemistryen
dc.contributor.institutionUniversita degli Studi di Roma La Sapienza, Rome, Italyen
dc.contributor.institutionUniversita di Salerno, Salerno, Italyen
kaust.grant.numberKUK-F1-036-32en

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