Short Oligonucleotides Aligned in Stretched Humid Matrix: Secondary DNA Structure in Poly(vinyl alcohol) Environment

Handle URI:
http://hdl.handle.net/10754/599360
Title:
Short Oligonucleotides Aligned in Stretched Humid Matrix: Secondary DNA Structure in Poly(vinyl alcohol) Environment
Authors:
Hanczyc, Piotr; Åkerman, Björn; Nordén, Bengt
Abstract:
We report that short, synthetic, double- as well as single-stranded DNA can be aligned in stretched humid poly(vinyl alcohol) (PVA) matrix, and the secondary structure (nucleobase orientation) can be characterized with linear dichroism (LD) spectroscopy. Oligonucleotides of lengths varying between 10 (3.4 nm) and 60 bases (20.4 nm) were investigated with respect to structural properties in the gel-like polymer environment. The DNA conformation as a function of relative humidity reveals a strong dependence of helical structure of DNA on PVA hydration level, results of relevance for nanotechnical studies of DNA-based supramolecular systems. Also, the PVA gel could provide possibilities to test models for nucleic acid interactions and distribution in cell contexts, including structural stability of genetic material in the cell and PVA-packaging for gene delivery. A method by which duplex oligonucleotides, with sequences designed to provide specific binding sites, become amenable to polarized-light spectroscopy opens up new possibilities for studying structure in DNA complexes with small adduct molecules as well as proteins. © 2012 American Chemical Society.
Citation:
Hanczyc P, Åkerman B, Nordén B (2012) Short Oligonucleotides Aligned in Stretched Humid Matrix: Secondary DNA Structure in Poly(vinyl alcohol) Environment. Langmuir 28: 6662–6669. Available: http://dx.doi.org/10.1021/la300960q.
Publisher:
American Chemical Society (ACS)
Journal:
Langmuir
Issue Date:
24-Apr-2012
DOI:
10.1021/la300960q
PubMed ID:
22452613
Type:
Article
ISSN:
0743-7463; 1520-5827
Sponsors:
We thank Bobo Feng for discussions and useful comments on the manuscript. The research was supported by grants from the King Abdullah University of Science and Technology (KAUST) and the European Research Council (ERC) to B.N.
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Full metadata record

DC FieldValue Language
dc.contributor.authorHanczyc, Piotren
dc.contributor.authorÅkerman, Björnen
dc.contributor.authorNordén, Bengten
dc.date.accessioned2016-02-28T06:05:32Zen
dc.date.available2016-02-28T06:05:32Zen
dc.date.issued2012-04-24en
dc.identifier.citationHanczyc P, Åkerman B, Nordén B (2012) Short Oligonucleotides Aligned in Stretched Humid Matrix: Secondary DNA Structure in Poly(vinyl alcohol) Environment. Langmuir 28: 6662–6669. Available: http://dx.doi.org/10.1021/la300960q.en
dc.identifier.issn0743-7463en
dc.identifier.issn1520-5827en
dc.identifier.pmid22452613en
dc.identifier.doi10.1021/la300960qen
dc.identifier.urihttp://hdl.handle.net/10754/599360en
dc.description.abstractWe report that short, synthetic, double- as well as single-stranded DNA can be aligned in stretched humid poly(vinyl alcohol) (PVA) matrix, and the secondary structure (nucleobase orientation) can be characterized with linear dichroism (LD) spectroscopy. Oligonucleotides of lengths varying between 10 (3.4 nm) and 60 bases (20.4 nm) were investigated with respect to structural properties in the gel-like polymer environment. The DNA conformation as a function of relative humidity reveals a strong dependence of helical structure of DNA on PVA hydration level, results of relevance for nanotechnical studies of DNA-based supramolecular systems. Also, the PVA gel could provide possibilities to test models for nucleic acid interactions and distribution in cell contexts, including structural stability of genetic material in the cell and PVA-packaging for gene delivery. A method by which duplex oligonucleotides, with sequences designed to provide specific binding sites, become amenable to polarized-light spectroscopy opens up new possibilities for studying structure in DNA complexes with small adduct molecules as well as proteins. © 2012 American Chemical Society.en
dc.description.sponsorshipWe thank Bobo Feng for discussions and useful comments on the manuscript. The research was supported by grants from the King Abdullah University of Science and Technology (KAUST) and the European Research Council (ERC) to B.N.en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleShort Oligonucleotides Aligned in Stretched Humid Matrix: Secondary DNA Structure in Poly(vinyl alcohol) Environmenten
dc.typeArticleen
dc.identifier.journalLangmuiren
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen
dc.contributor.institutionPolitechnika Wroclawska, Wroclaw, Polanden
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