Handle URI:
http://hdl.handle.net/10754/599358
Title:
Shear-Induced Membrane Fusion in Viscous Solutions
Authors:
Kogan, Maxim; Feng, Bobo; Nordén, Bengt; Rocha, Sandra; Beke-Somfai, Tamás
Abstract:
Large unilamellar lipid vesicles do not normally fuse under fluid shear stress. They might deform and open pores to relax the tension to which they are exposed, but membrane fusion occurring solely due to shear stress has not yet been reported. We present evidence that shear forces in a viscous solution can induce lipid bilayer fusion. The fusion of 1,2-dioleoyl-sn-glycero-3- phosphocholine (DOPC) liposomes is observed in Couette flow with shear rates above 3000 s-1 provided that the medium is viscous enough. Liposome samples, prepared at different viscosities using a 0-50 wt % range of sucrose concentration, were studied by dynamic light scattering, lipid fusion assays using Förster resonance energy transfer (FRET), and linear dichroism (LD) spectroscopy. Liposomes in solutions with 40 wt % (or more) sucrose showed lipid fusion under shear forces. These results support the hypothesis that under suitable conditions lipid membranes may fuse in response to mechanical-force- induced stress. © 2014 American Chemical Society.
Citation:
Kogan M, Feng B, Nordén B, Rocha S, Beke-Somfai T (2014) Shear-Induced Membrane Fusion in Viscous Solutions. Langmuir 30: 4875–4878. Available: http://dx.doi.org/10.1021/la404857r.
Publisher:
American Chemical Society (ACS)
Journal:
Langmuir
KAUST Grant Number:
KUK-11-008-23
Issue Date:
6-May-2014
DOI:
10.1021/la404857r
PubMed ID:
24758573
Type:
Article
ISSN:
0743-7463; 1520-5827
Sponsors:
This work was supported by King Abdullah University of Science and Technology (KAUST) (grant KUK-11-008-23), the European Research Council (EC-2008 AdG 227700-SUMO), and the Swedish Research Council - Linnaeus grant SUPRA 349-2007-8680.
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Full metadata record

DC FieldValue Language
dc.contributor.authorKogan, Maximen
dc.contributor.authorFeng, Boboen
dc.contributor.authorNordén, Bengten
dc.contributor.authorRocha, Sandraen
dc.contributor.authorBeke-Somfai, Tamásen
dc.date.accessioned2016-02-28T06:05:29Zen
dc.date.available2016-02-28T06:05:29Zen
dc.date.issued2014-05-06en
dc.identifier.citationKogan M, Feng B, Nordén B, Rocha S, Beke-Somfai T (2014) Shear-Induced Membrane Fusion in Viscous Solutions. Langmuir 30: 4875–4878. Available: http://dx.doi.org/10.1021/la404857r.en
dc.identifier.issn0743-7463en
dc.identifier.issn1520-5827en
dc.identifier.pmid24758573en
dc.identifier.doi10.1021/la404857ren
dc.identifier.urihttp://hdl.handle.net/10754/599358en
dc.description.abstractLarge unilamellar lipid vesicles do not normally fuse under fluid shear stress. They might deform and open pores to relax the tension to which they are exposed, but membrane fusion occurring solely due to shear stress has not yet been reported. We present evidence that shear forces in a viscous solution can induce lipid bilayer fusion. The fusion of 1,2-dioleoyl-sn-glycero-3- phosphocholine (DOPC) liposomes is observed in Couette flow with shear rates above 3000 s-1 provided that the medium is viscous enough. Liposome samples, prepared at different viscosities using a 0-50 wt % range of sucrose concentration, were studied by dynamic light scattering, lipid fusion assays using Förster resonance energy transfer (FRET), and linear dichroism (LD) spectroscopy. Liposomes in solutions with 40 wt % (or more) sucrose showed lipid fusion under shear forces. These results support the hypothesis that under suitable conditions lipid membranes may fuse in response to mechanical-force- induced stress. © 2014 American Chemical Society.en
dc.description.sponsorshipThis work was supported by King Abdullah University of Science and Technology (KAUST) (grant KUK-11-008-23), the European Research Council (EC-2008 AdG 227700-SUMO), and the Swedish Research Council - Linnaeus grant SUPRA 349-2007-8680.en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleShear-Induced Membrane Fusion in Viscous Solutionsen
dc.typeArticleen
dc.identifier.journalLangmuiren
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen
kaust.grant.numberKUK-11-008-23en

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