Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae

Handle URI:
http://hdl.handle.net/10754/599337
Title:
Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae
Authors:
Barbrook, Adrian C.; Dorrell, Richard G.; Burrows, Jennifer; Plenderleith, Lindsey J.; Nisbet, R. Ellen R.; Howe, Christopher J.
Abstract:
Although transcription and transcript processing in the chloroplasts of plants have been extensively characterised, the RNA metabolism of other chloroplast lineages across the eukaryotes remains poorly understood. In this paper, we use RT-PCR to study transcription and transcript processing in the chloroplasts of Amphidinium carterae, a model peridinin-containing dinoflagellate. These organisms have a highly unusual chloroplast genome, with genes located on multiple small 'minicircle' elements, and a number of idiosyncratic features of RNA metabolism including transcription via a rolling circle mechanism, and 3′ terminal polyuridylylation of transcripts. We demonstrate that transcription occurs in A. carterae via a rolling circle mechanism, as previously shown in the dinoflagellate Heterocapsa, and present evidence for the production of both polycistronic and monocistronic transcripts from A. carterae minicircles, including several regions containing ORFs previously not known to be expressed. We demonstrate the presence of both polyuridylylated and non-polyuridylylated transcripts in A. carterae, and show that polycistronic transcripts can be terminally polyuridylylated. We present a model for RNA metabolism in dinoflagellate chloroplasts where long polycistronic precursors are processed to form mature transcripts. Terminal polyuridylylation may mark transcripts with the correct 3′ end. © 2012 Springer Science+Business Media B.V.
Citation:
Barbrook AC, Dorrell RG, Burrows J, Plenderleith LJ, Nisbet RER, et al. (2012) Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae. Plant Molecular Biology 79: 347–357. Available: http://dx.doi.org/10.1007/s11103-012-9916-z.
Publisher:
Springer Nature
Journal:
Plant Molecular Biology
Issue Date:
5-May-2012
DOI:
10.1007/s11103-012-9916-z
PubMed ID:
22562591
Type:
Article
ISSN:
0167-4412; 1573-5028
Sponsors:
We thank the Leverhulme Trust (F/09 641/F), the Isaac Newton Trust (University of Cambridge), and the King Abdullah University of Science and Technology for financial support. R.G.D. was supported by a BBSRC PhD studentship, and C.J.H. by a University of South Australia Distinguished Researcher Award. We thank the anonymous referees for their helpful comments on the drafting of the manuscript.
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Full metadata record

DC FieldValue Language
dc.contributor.authorBarbrook, Adrian C.en
dc.contributor.authorDorrell, Richard G.en
dc.contributor.authorBurrows, Jenniferen
dc.contributor.authorPlenderleith, Lindsey J.en
dc.contributor.authorNisbet, R. Ellen R.en
dc.contributor.authorHowe, Christopher J.en
dc.date.accessioned2016-02-28T05:49:24Zen
dc.date.available2016-02-28T05:49:24Zen
dc.date.issued2012-05-05en
dc.identifier.citationBarbrook AC, Dorrell RG, Burrows J, Plenderleith LJ, Nisbet RER, et al. (2012) Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae. Plant Molecular Biology 79: 347–357. Available: http://dx.doi.org/10.1007/s11103-012-9916-z.en
dc.identifier.issn0167-4412en
dc.identifier.issn1573-5028en
dc.identifier.pmid22562591en
dc.identifier.doi10.1007/s11103-012-9916-zen
dc.identifier.urihttp://hdl.handle.net/10754/599337en
dc.description.abstractAlthough transcription and transcript processing in the chloroplasts of plants have been extensively characterised, the RNA metabolism of other chloroplast lineages across the eukaryotes remains poorly understood. In this paper, we use RT-PCR to study transcription and transcript processing in the chloroplasts of Amphidinium carterae, a model peridinin-containing dinoflagellate. These organisms have a highly unusual chloroplast genome, with genes located on multiple small 'minicircle' elements, and a number of idiosyncratic features of RNA metabolism including transcription via a rolling circle mechanism, and 3′ terminal polyuridylylation of transcripts. We demonstrate that transcription occurs in A. carterae via a rolling circle mechanism, as previously shown in the dinoflagellate Heterocapsa, and present evidence for the production of both polycistronic and monocistronic transcripts from A. carterae minicircles, including several regions containing ORFs previously not known to be expressed. We demonstrate the presence of both polyuridylylated and non-polyuridylylated transcripts in A. carterae, and show that polycistronic transcripts can be terminally polyuridylylated. We present a model for RNA metabolism in dinoflagellate chloroplasts where long polycistronic precursors are processed to form mature transcripts. Terminal polyuridylylation may mark transcripts with the correct 3′ end. © 2012 Springer Science+Business Media B.V.en
dc.description.sponsorshipWe thank the Leverhulme Trust (F/09 641/F), the Isaac Newton Trust (University of Cambridge), and the King Abdullah University of Science and Technology for financial support. R.G.D. was supported by a BBSRC PhD studentship, and C.J.H. by a University of South Australia Distinguished Researcher Award. We thank the anonymous referees for their helpful comments on the drafting of the manuscript.en
dc.publisherSpringer Natureen
dc.subjectChloroplasten
dc.subjectMinicirclesen
dc.subjectPolyUen
dc.subjectProtisten
dc.subjectTranscript processingen
dc.titlePolyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carteraeen
dc.typeArticleen
dc.identifier.journalPlant Molecular Biologyen
dc.contributor.institutionUniversity of Cambridge, Cambridge, United Kingdomen
dc.contributor.institutionUniversity of South Australia, Adelaide, Australiaen

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