On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis

Handle URI:
http://hdl.handle.net/10754/599071
Title:
On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis
Authors:
Li, Shunbo; Ye, Ziran; Hui, Yu Sanna; Gao, Yibo; Jiang, Yusheng; Wen, Weijia
Abstract:
© 2015 AIP Publishing LLC. Electrodeless dielectrophoresis is the best choice to achieve preconcentration of nanoparticles and biomolecules due to its simple, robust, and easy implementation. We designed a simple chip with microchannels and nano-slits in between and then studied the trapping of DNA in high conductive medium and low conductive medium, corresponding to positive and negative dielectrophoresis (DEP), respectively. It is very important to investigate the trapping in media with different conductivities since one always has to deal with the sample solutions with different conductivities. The trapping process was analyzed by the fluorescent intensity changes. The results showed that DNA could be trapped at the nano-slit in both high and low conductive media in a lower electric field strength (10 V/cm) compared to the existing methods. This is a significant improvement to suppress the Joule heating effect in DEP related experiments. Our work may give insight to researchers for DNA trapping by a simple and low cost device in the Lab-on-a-Chip system.
Citation:
Li S, Ye Z, Hui YS, Gao Y, Jiang Y, et al. (2015) On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis. Biomicrofluidics 9: 054115. Available: http://dx.doi.org/10.1063/1.4932177.
Publisher:
AIP Publishing
Journal:
Biomicrofluidics
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
Sep-2015
DOI:
10.1063/1.4932177
PubMed ID:
26487901
PubMed Central ID:
PMC4592423
Type:
Article
ISSN:
1932-1058
Sponsors:
The authors would like to thank Mr. Weipeng Zhang from Environmental Science Program, HKUST for providing DNA and the fruitful discussions. The authors acknowledge the financial support provided by the Hong Kong Research Grants Council (Grant Nos. HKUST604710 and 605411) and National Natural Science Foundation of China (Grant No. 11290165). This work was partially supported by Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Shunboen
dc.contributor.authorYe, Ziranen
dc.contributor.authorHui, Yu Sannaen
dc.contributor.authorGao, Yiboen
dc.contributor.authorJiang, Yushengen
dc.contributor.authorWen, Weijiaen
dc.date.accessioned2016-02-25T13:52:19Zen
dc.date.available2016-02-25T13:52:19Zen
dc.date.issued2015-09en
dc.identifier.citationLi S, Ye Z, Hui YS, Gao Y, Jiang Y, et al. (2015) On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis. Biomicrofluidics 9: 054115. Available: http://dx.doi.org/10.1063/1.4932177.en
dc.identifier.issn1932-1058en
dc.identifier.pmid26487901en
dc.identifier.doi10.1063/1.4932177en
dc.identifier.urihttp://hdl.handle.net/10754/599071en
dc.description.abstract© 2015 AIP Publishing LLC. Electrodeless dielectrophoresis is the best choice to achieve preconcentration of nanoparticles and biomolecules due to its simple, robust, and easy implementation. We designed a simple chip with microchannels and nano-slits in between and then studied the trapping of DNA in high conductive medium and low conductive medium, corresponding to positive and negative dielectrophoresis (DEP), respectively. It is very important to investigate the trapping in media with different conductivities since one always has to deal with the sample solutions with different conductivities. The trapping process was analyzed by the fluorescent intensity changes. The results showed that DNA could be trapped at the nano-slit in both high and low conductive media in a lower electric field strength (10 V/cm) compared to the existing methods. This is a significant improvement to suppress the Joule heating effect in DEP related experiments. Our work may give insight to researchers for DNA trapping by a simple and low cost device in the Lab-on-a-Chip system.en
dc.description.sponsorshipThe authors would like to thank Mr. Weipeng Zhang from Environmental Science Program, HKUST for providing DNA and the fruitful discussions. The authors acknowledge the financial support provided by the Hong Kong Research Grants Council (Grant Nos. HKUST604710 and 605411) and National Natural Science Foundation of China (Grant No. 11290165). This work was partially supported by Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST).en
dc.publisherAIP Publishingen
dc.titleOn-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresisen
dc.typeArticleen
dc.identifier.journalBiomicrofluidicsen
dc.identifier.pmcidPMC4592423en
dc.contributor.institutionDepartment of Physics and Nano Science and Technology Program, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.institutionSchool of Chemistry, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, United Kingdomen
dc.contributor.institutionEnvironmental Science Program, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.institutionCollege of Communication Engineering, Chongqing University, Chongqing 400044, Chinaen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
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