Novel lncRNAs in myogenesis: a miR-31 overlapping transcript controls myoblast differentiation.

Handle URI:
http://hdl.handle.net/10754/599009
Title:
Novel lncRNAs in myogenesis: a miR-31 overlapping transcript controls myoblast differentiation.
Authors:
Ballarino, Monica; Cazzella, Valentina; D'Andrea, Daniel; Grassi, Luigi; Bisceglie, Lavinia; Cipriano, Andrea; Santini, Tiziana; Pinnarò, Chiara; Morlando, Mariangela; Tramontano, Anna; Bozzoni, Irene
Abstract:
Transcriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.
Citation:
Ballarino M, Cazzella V, D’Andrea D, Grassi L, Bisceglie L, et al. (2014) Novel lncRNAs in myogenesis: a miR-31 overlapping transcript controls myoblast differentiation. . Mol Cell Biol 35: MCB.01394–14. Available: http://dx.doi.org/10.1128/MCB.01394-14.
Publisher:
American Society for Microbiology
Journal:
Molecular and Cellular Biology
Issue Date:
15-Dec-2014
DOI:
10.1128/MCB.01394-14
PubMed ID:
25512605
PubMed Central ID:
PMC4301723
Type:
Article
ISSN:
0270-7306; 1098-5549
Sponsors:
This work was partially supported by grants from ERC (ERC-2013-AdG340172-MUNCODD), Telethon (GGP11149), the Epigen-Epigenomics Flagship Project, Parent Project Italia, and PRIN to I.B. and KAUST and PRIN to A.T.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorBallarino, Monicaen
dc.contributor.authorCazzella, Valentinaen
dc.contributor.authorD'Andrea, Danielen
dc.contributor.authorGrassi, Luigien
dc.contributor.authorBisceglie, Laviniaen
dc.contributor.authorCipriano, Andreaen
dc.contributor.authorSantini, Tizianaen
dc.contributor.authorPinnarò, Chiaraen
dc.contributor.authorMorlando, Mariangelaen
dc.contributor.authorTramontano, Annaen
dc.contributor.authorBozzoni, Ireneen
dc.date.accessioned2016-02-25T13:51:06Zen
dc.date.available2016-02-25T13:51:06Zen
dc.date.issued2014-12-15en
dc.identifier.citationBallarino M, Cazzella V, D’Andrea D, Grassi L, Bisceglie L, et al. (2014) Novel lncRNAs in myogenesis: a miR-31 overlapping transcript controls myoblast differentiation. . Mol Cell Biol 35: MCB.01394–14. Available: http://dx.doi.org/10.1128/MCB.01394-14.en
dc.identifier.issn0270-7306en
dc.identifier.issn1098-5549en
dc.identifier.pmid25512605en
dc.identifier.doi10.1128/MCB.01394-14en
dc.identifier.urihttp://hdl.handle.net/10754/599009en
dc.description.abstractTranscriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.en
dc.description.sponsorshipThis work was partially supported by grants from ERC (ERC-2013-AdG340172-MUNCODD), Telethon (GGP11149), the Epigen-Epigenomics Flagship Project, Parent Project Italia, and PRIN to I.B. and KAUST and PRIN to A.T.en
dc.publisherAmerican Society for Microbiologyen
dc.titleNovel lncRNAs in myogenesis: a miR-31 overlapping transcript controls myoblast differentiation.en
dc.typeArticleen
dc.identifier.journalMolecular and Cellular Biologyen
dc.identifier.pmcidPMC4301723en
dc.contributor.institutionDept. of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Rome, Italy.en
dc.contributor.institutionDept. of Physics, Sapienza University of Rome, Rome, Italy.en
dc.contributor.institutionCenter for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.en
dc.contributor.institutionDept. of Physics, Sapienza University of Rome, Rome, Italy Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.en
dc.contributor.institutionDept. of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Rome, Italy Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy Institute Pasteur Fondazione Cenci-Bolognetti and IBPM, Sapienza University of Rome, Rome, Italy irene.bozzoni@uniroma1.it.en

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.