Modeling Stem/Progenitor Cell-Induced Neovascularization and Oxygenation Around Solid Implants

Handle URI:
http://hdl.handle.net/10754/598861
Title:
Modeling Stem/Progenitor Cell-Induced Neovascularization and Oxygenation Around Solid Implants
Authors:
Jain, Harsh Vardhan; Moldovan, Nicanor I.; Byrne, Helen M.
Abstract:
Tissue engineering constructs and other solid implants with biomedical applications, such as drug delivery devices or bioartificial organs, need oxygen (O(2)) to function properly. To understand better the vascular integration of such devices, we recently developed a novel model sensor containing O(2)-sensitive crystals, consisting of a polymeric capsule limited by a nanoporous filter. The sensor was implanted in mice with hydrogel alone (control) or hydrogel embedded with mouse CD117/c-kit+ bone marrow progenitor cells in order to stimulate peri-implant neovascularization. The sensor provided local partial O(2) pressure (pO(2)) using noninvasive electron paramagnetic resonance signal measurements. A consistently higher level of peri-implant oxygenation was observed in the cell-treatment case than in the control over a 10-week period. To provide a mechanistic explanation of these experimental observations, we present in this article a mathematical model, formulated as a system of coupled partial differential equations, that simulates peri-implant vascularization. In the control case, vascularization is considered to be the result of a foreign body reaction, while in the cell-treatment case, adipogenesis in response to paracrine stimuli produced by the stem cells is assumed to induce neovascularization. The model is validated by fitting numerical predictions of local pO(2) to measurements from the implanted sensor. The model is then used to investigate further the potential for using stem cell treatment to enhance the vascular integration of biomedical implants. We thus demonstrate how mathematical modeling combined with experimentation can be used to infer how vasculature develops around biomedical implants in control and stem cell-treated cases.
Citation:
Jain HV, Moldovan NI, Byrne HM (2012) Modeling Stem/Progenitor Cell-Induced Neovascularization and Oxygenation Around Solid Implants. Tissue Engineering Part C: Methods 18: 487–495. Available: http://dx.doi.org/10.1089/ten.tec.2011.0452.
Publisher:
Mary Ann Liebert Inc
Journal:
Tissue Engineering Part C: Methods
KAUST Grant Number:
KUK-C1-013-04
Issue Date:
Jul-2012
DOI:
10.1089/ten.tec.2011.0452
PubMed ID:
22224628
PubMed Central ID:
PMC3400277
Type:
Article
ISSN:
1937-3384; 1937-3392
Sponsors:
The authors thank Drs. Markus Owen and Holger Perfahl for many helpful discussions. This work was made possible in part by a U.S. partnering award from the Biotechnology and Biological Sciences Research Council (Grant BB/G530484/1), which facilitates collaboration between the University of Nottingham and the Mathematical Biosciences Institute. H.V.J. acknowledges support from the MBI and the National Science Foundation (Grant 0635561). N.I.M. was supported by National Institutes of Health Grants R01 HL065983 and R01 HL096524. This publication is based on work supported in part by Award No. KUK-C1-013-04, made by King Abdullah University of Science and Technology (KAUST).
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Full metadata record

DC FieldValue Language
dc.contributor.authorJain, Harsh Vardhanen
dc.contributor.authorMoldovan, Nicanor I.en
dc.contributor.authorByrne, Helen M.en
dc.date.accessioned2016-02-25T13:42:37Zen
dc.date.available2016-02-25T13:42:37Zen
dc.date.issued2012-07en
dc.identifier.citationJain HV, Moldovan NI, Byrne HM (2012) Modeling Stem/Progenitor Cell-Induced Neovascularization and Oxygenation Around Solid Implants. Tissue Engineering Part C: Methods 18: 487–495. Available: http://dx.doi.org/10.1089/ten.tec.2011.0452.en
dc.identifier.issn1937-3384en
dc.identifier.issn1937-3392en
dc.identifier.pmid22224628en
dc.identifier.doi10.1089/ten.tec.2011.0452en
dc.identifier.urihttp://hdl.handle.net/10754/598861en
dc.description.abstractTissue engineering constructs and other solid implants with biomedical applications, such as drug delivery devices or bioartificial organs, need oxygen (O(2)) to function properly. To understand better the vascular integration of such devices, we recently developed a novel model sensor containing O(2)-sensitive crystals, consisting of a polymeric capsule limited by a nanoporous filter. The sensor was implanted in mice with hydrogel alone (control) or hydrogel embedded with mouse CD117/c-kit+ bone marrow progenitor cells in order to stimulate peri-implant neovascularization. The sensor provided local partial O(2) pressure (pO(2)) using noninvasive electron paramagnetic resonance signal measurements. A consistently higher level of peri-implant oxygenation was observed in the cell-treatment case than in the control over a 10-week period. To provide a mechanistic explanation of these experimental observations, we present in this article a mathematical model, formulated as a system of coupled partial differential equations, that simulates peri-implant vascularization. In the control case, vascularization is considered to be the result of a foreign body reaction, while in the cell-treatment case, adipogenesis in response to paracrine stimuli produced by the stem cells is assumed to induce neovascularization. The model is validated by fitting numerical predictions of local pO(2) to measurements from the implanted sensor. The model is then used to investigate further the potential for using stem cell treatment to enhance the vascular integration of biomedical implants. We thus demonstrate how mathematical modeling combined with experimentation can be used to infer how vasculature develops around biomedical implants in control and stem cell-treated cases.en
dc.description.sponsorshipThe authors thank Drs. Markus Owen and Holger Perfahl for many helpful discussions. This work was made possible in part by a U.S. partnering award from the Biotechnology and Biological Sciences Research Council (Grant BB/G530484/1), which facilitates collaboration between the University of Nottingham and the Mathematical Biosciences Institute. H.V.J. acknowledges support from the MBI and the National Science Foundation (Grant 0635561). N.I.M. was supported by National Institutes of Health Grants R01 HL065983 and R01 HL096524. This publication is based on work supported in part by Award No. KUK-C1-013-04, made by King Abdullah University of Science and Technology (KAUST).en
dc.publisherMary Ann Liebert Incen
dc.subject.meshImplants, Experimentalen
dc.subject.meshNeovascularization, Physiologicen
dc.subject.meshModels, Theoreticalen
dc.titleModeling Stem/Progenitor Cell-Induced Neovascularization and Oxygenation Around Solid Implantsen
dc.typeArticleen
dc.identifier.journalTissue Engineering Part C: Methodsen
dc.identifier.pmcidPMC3400277en
dc.contributor.institutionMathematical Biosciences Institute, The Ohio State University, Columbus, OH 43210, USA. hjain@mbi.osu.eduen
kaust.grant.numberKUK-C1-013-04en

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