Membrane Protrusion Coarsening and Nanotubulation within Giant Unilamellar Vesicles

Handle URI:
http://hdl.handle.net/10754/598797
Title:
Membrane Protrusion Coarsening and Nanotubulation within Giant Unilamellar Vesicles
Authors:
Węgrzyn, Ilona; Jeffries, Gavin D. M.; Nagel, Birgit; Katterle, Martin; Gerrard, Simon R.; Brown, Tom; Orwar, Owe; Jesorka, Aldo
Abstract:
Hydrophobic side groups on a stimuli-responsive polymer, encapsulated within a single giant unilamellar vesicle, enable membrane attachment during compartment formation at elevated temperatures. We thermally modulated the vesicle through implementation of an IR laser via an optical fiber, enabling localized directed heating. Polymer-membrane interactions were monitored using confocal imaging techniques as subsequent membrane protrusions occurred and lipid nanotubes formed in response to the polymer hydrogel contraction. These nanotubes, bridging the vesicle membrane to the contracting hydrogel, were retained on the surface of the polymer compartment, where they were transformed into smaller vesicles in a process reminiscent of cellular endocytosis. This development of a synthetic vesicle system containing a stimuli-responsive polymer could lead to a new platform for studying inter/intramembrane transport through lipid nanotubes. © 2011 American Chemical Society.
Citation:
Węgrzyn I, Jeffries GDM, Nagel B, Katterle M, Gerrard SR, et al. (2011) Membrane Protrusion Coarsening and Nanotubulation within Giant Unilamellar Vesicles. Journal of the American Chemical Society 133: 18046–18049. Available: http://dx.doi.org/10.1021/ja207536a.
Publisher:
American Chemical Society (ACS)
Journal:
Journal of the American Chemical Society
Issue Date:
16-Nov-2011
DOI:
10.1021/ja207536a
PubMed ID:
21978148
Type:
Article
ISSN:
0002-7863; 1520-5126
Sponsors:
This research was supported by the Knut and Alice Wallenberg Foundation, the Swedish Research Council (VR), the Swedish Strategic Research Foundation (SSF), the European Research Council (ERC), and the Nordforsk Network for Dynamic Bio-membrane Research. S. R. G. acknowledges financial support from KAUST.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorWęgrzyn, Ilonaen
dc.contributor.authorJeffries, Gavin D. M.en
dc.contributor.authorNagel, Birgiten
dc.contributor.authorKatterle, Martinen
dc.contributor.authorGerrard, Simon R.en
dc.contributor.authorBrown, Tomen
dc.contributor.authorOrwar, Oween
dc.contributor.authorJesorka, Aldoen
dc.date.accessioned2016-02-25T13:41:25Zen
dc.date.available2016-02-25T13:41:25Zen
dc.date.issued2011-11-16en
dc.identifier.citationWęgrzyn I, Jeffries GDM, Nagel B, Katterle M, Gerrard SR, et al. (2011) Membrane Protrusion Coarsening and Nanotubulation within Giant Unilamellar Vesicles. Journal of the American Chemical Society 133: 18046–18049. Available: http://dx.doi.org/10.1021/ja207536a.en
dc.identifier.issn0002-7863en
dc.identifier.issn1520-5126en
dc.identifier.pmid21978148en
dc.identifier.doi10.1021/ja207536aen
dc.identifier.urihttp://hdl.handle.net/10754/598797en
dc.description.abstractHydrophobic side groups on a stimuli-responsive polymer, encapsulated within a single giant unilamellar vesicle, enable membrane attachment during compartment formation at elevated temperatures. We thermally modulated the vesicle through implementation of an IR laser via an optical fiber, enabling localized directed heating. Polymer-membrane interactions were monitored using confocal imaging techniques as subsequent membrane protrusions occurred and lipid nanotubes formed in response to the polymer hydrogel contraction. These nanotubes, bridging the vesicle membrane to the contracting hydrogel, were retained on the surface of the polymer compartment, where they were transformed into smaller vesicles in a process reminiscent of cellular endocytosis. This development of a synthetic vesicle system containing a stimuli-responsive polymer could lead to a new platform for studying inter/intramembrane transport through lipid nanotubes. © 2011 American Chemical Society.en
dc.description.sponsorshipThis research was supported by the Knut and Alice Wallenberg Foundation, the Swedish Research Council (VR), the Swedish Strategic Research Foundation (SSF), the European Research Council (ERC), and the Nordforsk Network for Dynamic Bio-membrane Research. S. R. G. acknowledges financial support from KAUST.en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleMembrane Protrusion Coarsening and Nanotubulation within Giant Unilamellar Vesiclesen
dc.typeArticleen
dc.identifier.journalJournal of the American Chemical Societyen
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen
dc.contributor.institutionFraunhofer-Institut fur Biomedizinische Technik - IBMT, St Ingbert, Germanyen
dc.contributor.institutionUniversity of Southampton, Southampton, United Kingdomen

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