Membrane biofouling characterization: effects of sample preparation procedures on biofilm structure and the microbial community

Handle URI:
http://hdl.handle.net/10754/598795
Title:
Membrane biofouling characterization: effects of sample preparation procedures on biofilm structure and the microbial community
Authors:
Xue, Zheng; Lu, Huijie; Liu, Wen-Tso
Abstract:
Ensuring the quality and reproducibility of results from biofilm structure and microbial community analysis is essential to membrane biofouling studies. This study evaluated the impacts of three sample preparation factors (ie number of buffer rinses, storage time at 4°C, and DNA extraction method) on the downstream analysis of nitrifying biofilms grown on ultrafiltration membranes. Both rinse and storage affected biofilm structure, as suggested by their strong correlation with total biovolume, biofilm thickness, roughness and the spatial distribution of EPS. Significant variations in DNA yields and microbial community diversity were also observed among samples treated by different rinses, storage and DNA extraction methods. For the tested biofilms, two rinses, no storage and DNA extraction with both mechanical and chemical cell lysis from attached biofilm were the optimal sample preparation procedures for obtaining accurate information about biofilm structure, EPS distribution and the microbial community. © 2014 © 2014 Taylor & Francis.
Citation:
Xue Z, Lu H, Liu W-T (2014) Membrane biofouling characterization: effects of sample preparation procedures on biofilm structure and the microbial community. Biofouling 30: 813–821. Available: http://dx.doi.org/10.1080/08927014.2014.935937.
Publisher:
Informa UK Limited
Journal:
Biofouling
Issue Date:
15-Jul-2014
DOI:
10.1080/08927014.2014.935937
PubMed ID:
25115516
Type:
Article
ISSN:
0892-7014; 1029-2454
Sponsors:
This study was supported by King Abdullah University of Science and Technology (KAUST, Thuwal, Saudi Arabia). The authors would like to thank Chris Wright at the University of Illinois at Urbana-Champaign Roy J. Carver Biotechnology Center for performing the Illumina MiSeq sequencing. The flow cells were kindly provided by Dr Peiying Hong and Moustapha Harb at the KAUST Water Desalination Reuse Center.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorXue, Zhengen
dc.contributor.authorLu, Huijieen
dc.contributor.authorLiu, Wen-Tsoen
dc.date.accessioned2016-02-25T13:41:22Zen
dc.date.available2016-02-25T13:41:22Zen
dc.date.issued2014-07-15en
dc.identifier.citationXue Z, Lu H, Liu W-T (2014) Membrane biofouling characterization: effects of sample preparation procedures on biofilm structure and the microbial community. Biofouling 30: 813–821. Available: http://dx.doi.org/10.1080/08927014.2014.935937.en
dc.identifier.issn0892-7014en
dc.identifier.issn1029-2454en
dc.identifier.pmid25115516en
dc.identifier.doi10.1080/08927014.2014.935937en
dc.identifier.urihttp://hdl.handle.net/10754/598795en
dc.description.abstractEnsuring the quality and reproducibility of results from biofilm structure and microbial community analysis is essential to membrane biofouling studies. This study evaluated the impacts of three sample preparation factors (ie number of buffer rinses, storage time at 4°C, and DNA extraction method) on the downstream analysis of nitrifying biofilms grown on ultrafiltration membranes. Both rinse and storage affected biofilm structure, as suggested by their strong correlation with total biovolume, biofilm thickness, roughness and the spatial distribution of EPS. Significant variations in DNA yields and microbial community diversity were also observed among samples treated by different rinses, storage and DNA extraction methods. For the tested biofilms, two rinses, no storage and DNA extraction with both mechanical and chemical cell lysis from attached biofilm were the optimal sample preparation procedures for obtaining accurate information about biofilm structure, EPS distribution and the microbial community. © 2014 © 2014 Taylor & Francis.en
dc.description.sponsorshipThis study was supported by King Abdullah University of Science and Technology (KAUST, Thuwal, Saudi Arabia). The authors would like to thank Chris Wright at the University of Illinois at Urbana-Champaign Roy J. Carver Biotechnology Center for performing the Illumina MiSeq sequencing. The flow cells were kindly provided by Dr Peiying Hong and Moustapha Harb at the KAUST Water Desalination Reuse Center.en
dc.publisherInforma UK Limiteden
dc.subjectbiofilm sample preparationen
dc.subjectbiofoulingen
dc.subjectconfocal microscopyen
dc.subjectextracellular polymeric substancesen
dc.subjectMiseq sequencingen
dc.titleMembrane biofouling characterization: effects of sample preparation procedures on biofilm structure and the microbial communityen
dc.typeArticleen
dc.identifier.journalBiofoulingen
dc.contributor.institutionUniversity of Illinois at Urbana-Champaign, Urbana, United Statesen

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