Meiotic genes and sexual reproduction in the green algal class Trebouxiophyceae (Chlorophyta)

Handle URI:
http://hdl.handle.net/10754/598794
Title:
Meiotic genes and sexual reproduction in the green algal class Trebouxiophyceae (Chlorophyta)
Authors:
Fučíková, Karolina; Pažoutová, Marie; Rindi, Fabio
Abstract:
© 2015 Phycological Society of America. Sexual reproduction is widespread in eukaryotes and is well documented in chlorophytan green algae. In this lineage, however, the Trebouxiophyceae represent a striking exception: in contrast to its relatives Chlorophyceae and Ulvophyceae this group appears to be mostly asexual, as fertilization has been rarely observed. Assessments of sexual reproduction in the Trebouxiophyceae have been based on microscopic observation of gametes fusing. New genomic data offer now the opportunity to check for the presence of meiotic genes, which represent an indirect evidence of a sexual life cycle. Using genomic and transcriptomic data for 12 taxa spanning the phylogenetic breadth of the class, we tried to clarify whether genuine asexuality or cryptic sexuality is the most likely case for the numerous putatively asexual trebouxiophytes. On the basis of these data and a bibliographic review, we conclude that the view of trebouxiophytes as primarily asexual is incorrect. In contrast to the limited number of reports of fertilization, meiotic genes were found in all genomes and transcriptomes examined, even in species presumed asexual. In the taxa examined the totality or majority of the genes were present, Helicosporidium and Auxenochlorella being the only partial exceptions (only four genes present). The evidence of sex provided by the meiotic genes is phylogenetically widespread in the class and indicates that sexual reproduction is not associated with any particular morphological or ecological trait. On the basis of the results, we expect that the existence of the meiotic genes will be documented in all trebouxiophycean genomes that will become available in the future.
Citation:
Fučíková K, Pažoutová M, Rindi F (2015) Meiotic genes and sexual reproduction in the green algal class Trebouxiophyceae (Chlorophyta). J Phycol 51: 419–430. Available: http://dx.doi.org/10.1111/jpy.12293.
Publisher:
Wiley-Blackwell
Journal:
Journal of Phycology
KAUST Grant Number:
IC/2010/09
Issue Date:
6-Apr-2015
DOI:
10.1111/jpy.12293
Type:
Article
ISSN:
0022-3646
Sponsors:
We are thankful to Guillaume Blanc for consultation, Lenka Štenclová, Erik Birčák and Aleš Ogar Horák for help, and Míra Oborník for generous support. Our gratitude goes to Stephane Rombauts and Yves van de Peer and his lab members for kind help. M.P. was supported by the Award IC/2010/09 by the King Abdullah University of Science and Technology (KAUST). K.F. is thankful for support to Louise Lewis and Paul Lewis through NSF grant DEB-1036448, which also funded the genomic data collection, and Endymion Cooper and Charles Delwiche for providing access to the Watanabea reniformis and Prasiolopsis sp. transcriptomic data (funded by NSF DEB-1036506). Genome assemblies and BLAST searches used the computational resources provided by the Bioinformatics Facility of the University of Connecticut Biotechnology and Bioservices Center as well as resources hosted by the University of Maryland. We are very grateful to Pierre Rouzé, Heroen Verbruggen and Pavel Škaloud for stimulating discussion and suggestions that helped to improve the manuscript.
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Full metadata record

DC FieldValue Language
dc.contributor.authorFučíková, Karolinaen
dc.contributor.authorPažoutová, Marieen
dc.contributor.authorRindi, Fabioen
dc.date.accessioned2016-02-25T13:41:21Zen
dc.date.available2016-02-25T13:41:21Zen
dc.date.issued2015-04-06en
dc.identifier.citationFučíková K, Pažoutová M, Rindi F (2015) Meiotic genes and sexual reproduction in the green algal class Trebouxiophyceae (Chlorophyta). J Phycol 51: 419–430. Available: http://dx.doi.org/10.1111/jpy.12293.en
dc.identifier.issn0022-3646en
dc.identifier.doi10.1111/jpy.12293en
dc.identifier.urihttp://hdl.handle.net/10754/598794en
dc.description.abstract© 2015 Phycological Society of America. Sexual reproduction is widespread in eukaryotes and is well documented in chlorophytan green algae. In this lineage, however, the Trebouxiophyceae represent a striking exception: in contrast to its relatives Chlorophyceae and Ulvophyceae this group appears to be mostly asexual, as fertilization has been rarely observed. Assessments of sexual reproduction in the Trebouxiophyceae have been based on microscopic observation of gametes fusing. New genomic data offer now the opportunity to check for the presence of meiotic genes, which represent an indirect evidence of a sexual life cycle. Using genomic and transcriptomic data for 12 taxa spanning the phylogenetic breadth of the class, we tried to clarify whether genuine asexuality or cryptic sexuality is the most likely case for the numerous putatively asexual trebouxiophytes. On the basis of these data and a bibliographic review, we conclude that the view of trebouxiophytes as primarily asexual is incorrect. In contrast to the limited number of reports of fertilization, meiotic genes were found in all genomes and transcriptomes examined, even in species presumed asexual. In the taxa examined the totality or majority of the genes were present, Helicosporidium and Auxenochlorella being the only partial exceptions (only four genes present). The evidence of sex provided by the meiotic genes is phylogenetically widespread in the class and indicates that sexual reproduction is not associated with any particular morphological or ecological trait. On the basis of the results, we expect that the existence of the meiotic genes will be documented in all trebouxiophycean genomes that will become available in the future.en
dc.description.sponsorshipWe are thankful to Guillaume Blanc for consultation, Lenka Štenclová, Erik Birčák and Aleš Ogar Horák for help, and Míra Oborník for generous support. Our gratitude goes to Stephane Rombauts and Yves van de Peer and his lab members for kind help. M.P. was supported by the Award IC/2010/09 by the King Abdullah University of Science and Technology (KAUST). K.F. is thankful for support to Louise Lewis and Paul Lewis through NSF grant DEB-1036448, which also funded the genomic data collection, and Endymion Cooper and Charles Delwiche for providing access to the Watanabea reniformis and Prasiolopsis sp. transcriptomic data (funded by NSF DEB-1036506). Genome assemblies and BLAST searches used the computational resources provided by the Bioinformatics Facility of the University of Connecticut Biotechnology and Bioservices Center as well as resources hosted by the University of Maryland. We are very grateful to Pierre Rouzé, Heroen Verbruggen and Pavel Škaloud for stimulating discussion and suggestions that helped to improve the manuscript.en
dc.publisherWiley-Blackwellen
dc.subjectAlgal genomesen
dc.subjectChlorophytaen
dc.subjectGreen algaeen
dc.subjectMeiotic genesen
dc.subjectSexual reproductionen
dc.subjectTrebouxiophyceaeen
dc.titleMeiotic genes and sexual reproduction in the green algal class Trebouxiophyceae (Chlorophyta)en
dc.typeArticleen
dc.identifier.journalJournal of Phycologyen
dc.contributor.institutionDepartment of Ecology and Evolutionary Biology; University of Connecticut; 75 North Eagleville Road Storrs Connecticut 06269-3043 USAen
dc.contributor.institutionDepartment of Botany; Faculty of Science; University of South Bohemia; Ceske Budejovice 37005 Czech Republicen
dc.contributor.institutionInstitute of Parasitology; Biology Centre AS CR; Ceske Budejovice 37005 Czech Republicen
dc.contributor.institutionDipartimento di Scienze della Vita e dell'Ambiente; Università Politecnica delle Marche; Via Brecce Bianche Ancona 60131 Italyen
kaust.grant.numberIC/2010/09en
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