Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

Handle URI:
http://hdl.handle.net/10754/598750
Title:
Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy
Authors:
Nappini, Silvia; Al Kayal, Tamer; Berti, Debora; Nordèn, Bengt; Baglioni, Piero
Abstract:
Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMF). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO 2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO 2(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse. © 2011 American Chemical Society.
Citation:
Nappini S, Al Kayal T, Berti D, Nordèn B, Baglioni P (2011) Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy. The Journal of Physical Chemistry Letters 2: 713–718. Available: http://dx.doi.org/10.1021/jz2000936.
Publisher:
American Chemical Society (ACS)
Journal:
The Journal of Physical Chemistry Letters
Issue Date:
7-Apr-2011
DOI:
10.1021/jz2000936
Type:
Article
ISSN:
1948-7185
Sponsors:
Financial support from CSGI, MIUR-PRIN (2008-20087K9A2J), and FIRB (RBPR05JH2P ITALNANONET) is acknowledged. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr. Tore Eriksson is acknowledged for building the alternating magnetic field generator.
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Full metadata record

DC FieldValue Language
dc.contributor.authorNappini, Silviaen
dc.contributor.authorAl Kayal, Tameren
dc.contributor.authorBerti, Deboraen
dc.contributor.authorNordèn, Bengten
dc.contributor.authorBaglioni, Pieroen
dc.date.accessioned2016-02-25T13:40:28Zen
dc.date.available2016-02-25T13:40:28Zen
dc.date.issued2011-04-07en
dc.identifier.citationNappini S, Al Kayal T, Berti D, Nordèn B, Baglioni P (2011) Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy. The Journal of Physical Chemistry Letters 2: 713–718. Available: http://dx.doi.org/10.1021/jz2000936.en
dc.identifier.issn1948-7185en
dc.identifier.doi10.1021/jz2000936en
dc.identifier.urihttp://hdl.handle.net/10754/598750en
dc.description.abstractMagnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMF). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO 2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO 2(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse. © 2011 American Chemical Society.en
dc.description.sponsorshipFinancial support from CSGI, MIUR-PRIN (2008-20087K9A2J), and FIRB (RBPR05JH2P ITALNANONET) is acknowledged. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr. Tore Eriksson is acknowledged for building the alternating magnetic field generator.en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleMagnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopyen
dc.typeArticleen
dc.identifier.journalThe Journal of Physical Chemistry Lettersen
dc.contributor.institutionUniversita degli Studi di Firenze, Florence, Italyen
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen
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