Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin

Handle URI:
http://hdl.handle.net/10754/598689
Title:
Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin
Authors:
Quitterer, Felix; List, Anja; Eisenreich, Wolfgang; Bacher, Adelbert; Groll, Michael
Abstract:
Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.
Citation:
Quitterer F, List A, Eisenreich W, Bacher A, Groll M (2011) Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin. Angew Chem 124: 1367–1370. Available: http://dx.doi.org/10.1002/ange.201106765.
Publisher:
Wiley-Blackwell
Journal:
Angewandte Chemie
KAUST Grant Number:
FIC/2010/07
Issue Date:
16-Nov-2011
DOI:
10.1002/ange.201106765
Type:
Article
ISSN:
0044-8249
Sponsors:
Wir danken Sophie Vieweg fur ihre experimentelle Unterstutzung und den Mitarbeitern der PXI des Paul Scherrer Instituts, Swiss Light Source (Villigen, Schweiz) fur ihre Hilfe beim Messen der Datensutze. Diese Arbeit wurde von der Hans-Fischer-Gesellschaft und der King Abdullah University of Science and Technology (Award No. FIC/2010/07) gefçrdert.
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Full metadata record

DC FieldValue Language
dc.contributor.authorQuitterer, Felixen
dc.contributor.authorList, Anjaen
dc.contributor.authorEisenreich, Wolfgangen
dc.contributor.authorBacher, Adelberten
dc.contributor.authorGroll, Michaelen
dc.date.accessioned2016-02-25T13:34:29Zen
dc.date.available2016-02-25T13:34:29Zen
dc.date.issued2011-11-16en
dc.identifier.citationQuitterer F, List A, Eisenreich W, Bacher A, Groll M (2011) Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin. Angew Chem 124: 1367–1370. Available: http://dx.doi.org/10.1002/ange.201106765.en
dc.identifier.issn0044-8249en
dc.identifier.doi10.1002/ange.201106765en
dc.identifier.urihttp://hdl.handle.net/10754/598689en
dc.description.abstractMade by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.en
dc.description.sponsorshipWir danken Sophie Vieweg fur ihre experimentelle Unterstutzung und den Mitarbeitern der PXI des Paul Scherrer Instituts, Swiss Light Source (Villigen, Schweiz) fur ihre Hilfe beim Messen der Datensutze. Diese Arbeit wurde von der Hans-Fischer-Gesellschaft und der King Abdullah University of Science and Technology (Award No. FIC/2010/07) gefçrdert.en
dc.publisherWiley-Blackwellen
dc.titleKristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysinen
dc.typeArticleen
dc.identifier.journalAngewandte Chemieen
dc.contributor.institutionCenter for Integrated Protein Science at the Department Chemie Lehrstuhl fur Biochemie, Technische Universitat Munchen Lichtenbergstrasse 4, 85747 Garching (Germany)en
kaust.grant.numberFIC/2010/07en
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