Interactions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement

Handle URI:
http://hdl.handle.net/10754/598642
Title:
Interactions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement
Authors:
Gutierrez, Leonardo; Nguyen, Thanh H.
Abstract:
Interactions between rotavirus and Suwannee River natural organic matter (NOM) were studied by time-resolved dynamic light scattering, quartz crystal microbalance, and atomic force microscopy. In NOM-containing NaCl solutions of up to 600 mM, rotavirus suspension remained stable for over 4 h. Atomic force microscopy (AFM) measurement for interaction force decay length at different ionic strengths showed that nonelectrostatic repulsive forces were mainly responsible for eliminating aggregation in NaCl solutions. Aggregation rates of rotavirus in solutions containing 20 mg C/L increased with divalent cation concentration until reaching a critical coagulation concentration of 30 mM CaCl2 or 70 mM MgCl2. Deposition kinetics of rotavirus on NOM-coated silica surface was studied using quartz crystal microbalance. Experimental attachment efficiencies for rotavirus adsorption to NOM-coated surface in MgCl2 solution were lower than in CaCl2 solution at a given divalent cation concentration. Stronger adhesion force was measured for virus-virus and virus-NOM interactions in CaCl2 solution compared to those in MgCl2 or NaCl solutions at the same ionic strength. This study suggested that divalent cation complexation with carboxylate groups in NOM and on virus surface was an important mechanism in the deposition and aggregation kinetics of rotavirus. © 2012 American Chemical Society.
Citation:
Gutierrez L, Nguyen TH (2012) Interactions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement. Environ Sci Technol 46: 8705–8713. Available: http://dx.doi.org/10.1021/es301336u.
Publisher:
American Chemical Society (ACS)
Journal:
Environmental Science & Technology
Issue Date:
21-Aug-2012
DOI:
10.1021/es301336u
PubMed ID:
22834686
Type:
Article
ISSN:
0013-936X; 1520-5851
Sponsors:
This work was partially supported by NSF #0954501, the Academic Excellence Alliance program at King Abdullah University of Science and Technology, and the U.S. Department of Energy DE-FG02-07ER46453 and DE-FG02-07ER46471. We also acknowledge Dr. Scott McLaren, Ms. Ofelia Romero, and Mr. Tony Straub.
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Full metadata record

DC FieldValue Language
dc.contributor.authorGutierrez, Leonardoen
dc.contributor.authorNguyen, Thanh H.en
dc.date.accessioned2016-02-25T13:33:40Zen
dc.date.available2016-02-25T13:33:40Zen
dc.date.issued2012-08-21en
dc.identifier.citationGutierrez L, Nguyen TH (2012) Interactions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement. Environ Sci Technol 46: 8705–8713. Available: http://dx.doi.org/10.1021/es301336u.en
dc.identifier.issn0013-936Xen
dc.identifier.issn1520-5851en
dc.identifier.pmid22834686en
dc.identifier.doi10.1021/es301336uen
dc.identifier.urihttp://hdl.handle.net/10754/598642en
dc.description.abstractInteractions between rotavirus and Suwannee River natural organic matter (NOM) were studied by time-resolved dynamic light scattering, quartz crystal microbalance, and atomic force microscopy. In NOM-containing NaCl solutions of up to 600 mM, rotavirus suspension remained stable for over 4 h. Atomic force microscopy (AFM) measurement for interaction force decay length at different ionic strengths showed that nonelectrostatic repulsive forces were mainly responsible for eliminating aggregation in NaCl solutions. Aggregation rates of rotavirus in solutions containing 20 mg C/L increased with divalent cation concentration until reaching a critical coagulation concentration of 30 mM CaCl2 or 70 mM MgCl2. Deposition kinetics of rotavirus on NOM-coated silica surface was studied using quartz crystal microbalance. Experimental attachment efficiencies for rotavirus adsorption to NOM-coated surface in MgCl2 solution were lower than in CaCl2 solution at a given divalent cation concentration. Stronger adhesion force was measured for virus-virus and virus-NOM interactions in CaCl2 solution compared to those in MgCl2 or NaCl solutions at the same ionic strength. This study suggested that divalent cation complexation with carboxylate groups in NOM and on virus surface was an important mechanism in the deposition and aggregation kinetics of rotavirus. © 2012 American Chemical Society.en
dc.description.sponsorshipThis work was partially supported by NSF #0954501, the Academic Excellence Alliance program at King Abdullah University of Science and Technology, and the U.S. Department of Energy DE-FG02-07ER46453 and DE-FG02-07ER46471. We also acknowledge Dr. Scott McLaren, Ms. Ofelia Romero, and Mr. Tony Straub.en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleInteractions between Rotavirus and Suwannee River Organic Matter: Aggregation, Deposition, and Adhesion Force Measurementen
dc.typeArticleen
dc.identifier.journalEnvironmental Science & Technologyen
dc.contributor.institutionUniversity of Illinois at Urbana-Champaign, Urbana, United Statesen
kaust.grant.programAcademic Excellence Alliance (AEA)en
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