Impact of Chloramination on the Development of Laboratory-Grown Biofilms Fed with Filter-Pretreated Groundwater

Handle URI:
http://hdl.handle.net/10754/598561
Title:
Impact of Chloramination on the Development of Laboratory-Grown Biofilms Fed with Filter-Pretreated Groundwater
Authors:
Ling, Fangqiong; Liu, Wen-Tso
Abstract:
This study evaluated the continuous impact of monochloramine disinfection on laboratory-grown biofilms through the characterization of biofilm architecture and microbial community structure. Biofilm development and disinfection were achieved using CDC (Centers for Disease Control and Prevention) biofilm reactor systems with polyvinyl chloride (PVC) coupons as the substratum and sand filter-pretreated groundwater as the source of microbial seeding and growth nutrient. After 2 weeks of growth, the biofilms were subjected to chloramination for 8 more weeks at concentrations of 7.5±1.4 to 9.1±0.4 mg Cl2 L-1. Control reactors received no disinfection during the development of biofilms. Confocal laser scanning microscopy and image analysis indicated that chloramination could lead to 81.4-83.5% and 86.3-95.6% reduction in biofilm biomass and thickness, respectively, but could not eliminate biofilm growth. 16S rRNA gene terminal restriction fragment length polymorphism analysis indicated that microbial community structures between chloraminated and non-chloraminated biofilms exhibited different successional trends. 16S rRNA gene pyrosequencing analysis further revealed that chloramination could select members of Actinobacteria and Acidobacteria as the dominant populations, whereas natural development leads to the selection of members of Nitrospira and Bacteroidetes as dominant biofilm populations. Overall, chloramination treatment could alter the growth of multi-species biofilms on the PVC surface, shape the biofilm architecture, and select a certain microbial community that can survive or proliferate under chloramination.
Citation:
Ling F, Liu W-T (2013) Impact of Chloramination on the Development of Laboratory-Grown Biofilms Fed with Filter-Pretreated Groundwater. Microbes Environ 28: 50–57. Available: http://dx.doi.org/10.1264/jsme2.ME12095.
Publisher:
Japanese Society of Microbial Ecology
Journal:
Microbes and Environments
Issue Date:
2013
DOI:
10.1264/jsme2.ME12095
Type:
Article
ISSN:
1342-6311; 1347-4405
Sponsors:
This work was supported by a joint program between KAUST and the University of Illinois, Urbana-Champaign, under a KAUST-Academic Excellence Alliance (AEA) Grant. Fangqiong Ling was supported by a University of Illinois at Urbana-Champaign Graduate Fellowship.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorLing, Fangqiongen
dc.contributor.authorLiu, Wen-Tsoen
dc.date.accessioned2016-02-25T13:32:11Zen
dc.date.available2016-02-25T13:32:11Zen
dc.date.issued2013en
dc.identifier.citationLing F, Liu W-T (2013) Impact of Chloramination on the Development of Laboratory-Grown Biofilms Fed with Filter-Pretreated Groundwater. Microbes Environ 28: 50–57. Available: http://dx.doi.org/10.1264/jsme2.ME12095.en
dc.identifier.issn1342-6311en
dc.identifier.issn1347-4405en
dc.identifier.doi10.1264/jsme2.ME12095en
dc.identifier.urihttp://hdl.handle.net/10754/598561en
dc.description.abstractThis study evaluated the continuous impact of monochloramine disinfection on laboratory-grown biofilms through the characterization of biofilm architecture and microbial community structure. Biofilm development and disinfection were achieved using CDC (Centers for Disease Control and Prevention) biofilm reactor systems with polyvinyl chloride (PVC) coupons as the substratum and sand filter-pretreated groundwater as the source of microbial seeding and growth nutrient. After 2 weeks of growth, the biofilms were subjected to chloramination for 8 more weeks at concentrations of 7.5±1.4 to 9.1±0.4 mg Cl2 L-1. Control reactors received no disinfection during the development of biofilms. Confocal laser scanning microscopy and image analysis indicated that chloramination could lead to 81.4-83.5% and 86.3-95.6% reduction in biofilm biomass and thickness, respectively, but could not eliminate biofilm growth. 16S rRNA gene terminal restriction fragment length polymorphism analysis indicated that microbial community structures between chloraminated and non-chloraminated biofilms exhibited different successional trends. 16S rRNA gene pyrosequencing analysis further revealed that chloramination could select members of Actinobacteria and Acidobacteria as the dominant populations, whereas natural development leads to the selection of members of Nitrospira and Bacteroidetes as dominant biofilm populations. Overall, chloramination treatment could alter the growth of multi-species biofilms on the PVC surface, shape the biofilm architecture, and select a certain microbial community that can survive or proliferate under chloramination.en
dc.description.sponsorshipThis work was supported by a joint program between KAUST and the University of Illinois, Urbana-Champaign, under a KAUST-Academic Excellence Alliance (AEA) Grant. Fangqiong Ling was supported by a University of Illinois at Urbana-Champaign Graduate Fellowship.en
dc.publisherJapanese Society of Microbial Ecologyen
dc.subject16S rRNA pyrosequencingen
dc.subjectBiofilmen
dc.subjectChloramineen
dc.subjectDrinking wateren
dc.titleImpact of Chloramination on the Development of Laboratory-Grown Biofilms Fed with Filter-Pretreated Groundwateren
dc.typeArticleen
dc.identifier.journalMicrobes and Environmentsen
dc.contributor.institutionUniversity of Illinois at Urbana-Champaign, Urbana, United Statesen
kaust.grant.programAcademic Excellence Alliance (AEA)en
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