Global metabolomic responses of Nitrosomonas europaea 19718 to cold stress and altered ammonia feeding patterns

Handle URI:
http://hdl.handle.net/10754/598413
Title:
Global metabolomic responses of Nitrosomonas europaea 19718 to cold stress and altered ammonia feeding patterns
Authors:
Lu, Huijie; Ulanov, Alexander V.; Nobu, Masaru; Liu, Wen-Tso
Abstract:
© 2015 Springer-Verlag Berlin Heidelberg The model ammonia-oxidizing bacterium Nitrosomonas europaea represents one of the environmentally and biotechnologically significant microorganisms. Genome-based studies over the last decade have led to many intriguing discoveries about its cellular biochemistry and physiology. However, knowledge regarding the regulation of overall metabolic routes in response to various environmental stresses is limited due to a lack of comprehensive, time-resolved metabolomic analyses. In this study, gas chromatography–mass spectrometry (GC-MS)-based metabolic profiling was performed to characterize the temporal variations of N. europaea 19718 intercellular metabolites in response to varied temperature (23 and 10 °C) and ammonia feeding patterns (shock loading and continuous feeding of 20 mg N/L). Approximately 87 metabolites were successfully identified and mapped to the existing pathways of N. europaea 19718, allowing interpretation of the influence of temperature and feeding pattern on metabolite levels. In general, varied temperature had a more profound influence on the overall metabolism than varied feeding patterns. Total extracellular metabolite concentrations (relative to internal standards and normalized to biomass weight) were lower under cold stress and shock loading conditions compared with the control (continuous feeding at 23 °C). Cold stress caused the widespread downregulation of metabolites involved in central carbon metabolism, amino acid, and lipid synthesis (e.g., malonic acid, succinic acid, putrescine, and phosphonolpyruvate). Metabolites that showed differences under varied feeding patterns were mainly involved in nucleotide acid, amino acid, and lipid metabolism (e.g., adenine, uracil, and spermidine). This study highlighted the roles of central carbon and nitrogen metabolism in countering cold stress and altered ammonia availability. In addition, transcriptomic, proteomic, and metabolomic data from three studies on N. europaea were compared to achieve a holistic view of some important synergy and interconnectivity among different cellular components (RNA, protein, and metabolites) during ammonia starvation.
Citation:
Lu H, Ulanov AV, Nobu M, Liu W-T (2015) Global metabolomic responses of Nitrosomonas europaea 19718 to cold stress and altered ammonia feeding patterns. Applied Microbiology and Biotechnology 100: 1843–1852. Available: http://dx.doi.org/10.1007/s00253-015-7095-y.
Publisher:
Springer Science + Business Media
Journal:
Applied Microbiology and Biotechnology
Issue Date:
5-Nov-2015
DOI:
10.1007/s00253-015-7095-y
PubMed ID:
26536877
Type:
Article
ISSN:
0175-7598; 1432-0614
Sponsors:
This study was supported by King Abdullah University of Science and Technology (KAUST, Thuwal, Saudi Arabia). Authors would like to thank Yifan He at the Department of Civil and Environmental Engineering, University of Illinois-Urbana Champaign for experimental assistance.
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorLu, Huijieen
dc.contributor.authorUlanov, Alexander V.en
dc.contributor.authorNobu, Masaruen
dc.contributor.authorLiu, Wen-Tsoen
dc.date.accessioned2016-02-25T13:20:17Zen
dc.date.available2016-02-25T13:20:17Zen
dc.date.issued2015-11-05en
dc.identifier.citationLu H, Ulanov AV, Nobu M, Liu W-T (2015) Global metabolomic responses of Nitrosomonas europaea 19718 to cold stress and altered ammonia feeding patterns. Applied Microbiology and Biotechnology 100: 1843–1852. Available: http://dx.doi.org/10.1007/s00253-015-7095-y.en
dc.identifier.issn0175-7598en
dc.identifier.issn1432-0614en
dc.identifier.pmid26536877en
dc.identifier.doi10.1007/s00253-015-7095-yen
dc.identifier.urihttp://hdl.handle.net/10754/598413en
dc.description.abstract© 2015 Springer-Verlag Berlin Heidelberg The model ammonia-oxidizing bacterium Nitrosomonas europaea represents one of the environmentally and biotechnologically significant microorganisms. Genome-based studies over the last decade have led to many intriguing discoveries about its cellular biochemistry and physiology. However, knowledge regarding the regulation of overall metabolic routes in response to various environmental stresses is limited due to a lack of comprehensive, time-resolved metabolomic analyses. In this study, gas chromatography–mass spectrometry (GC-MS)-based metabolic profiling was performed to characterize the temporal variations of N. europaea 19718 intercellular metabolites in response to varied temperature (23 and 10 °C) and ammonia feeding patterns (shock loading and continuous feeding of 20 mg N/L). Approximately 87 metabolites were successfully identified and mapped to the existing pathways of N. europaea 19718, allowing interpretation of the influence of temperature and feeding pattern on metabolite levels. In general, varied temperature had a more profound influence on the overall metabolism than varied feeding patterns. Total extracellular metabolite concentrations (relative to internal standards and normalized to biomass weight) were lower under cold stress and shock loading conditions compared with the control (continuous feeding at 23 °C). Cold stress caused the widespread downregulation of metabolites involved in central carbon metabolism, amino acid, and lipid synthesis (e.g., malonic acid, succinic acid, putrescine, and phosphonolpyruvate). Metabolites that showed differences under varied feeding patterns were mainly involved in nucleotide acid, amino acid, and lipid metabolism (e.g., adenine, uracil, and spermidine). This study highlighted the roles of central carbon and nitrogen metabolism in countering cold stress and altered ammonia availability. In addition, transcriptomic, proteomic, and metabolomic data from three studies on N. europaea were compared to achieve a holistic view of some important synergy and interconnectivity among different cellular components (RNA, protein, and metabolites) during ammonia starvation.en
dc.description.sponsorshipThis study was supported by King Abdullah University of Science and Technology (KAUST, Thuwal, Saudi Arabia). Authors would like to thank Yifan He at the Department of Civil and Environmental Engineering, University of Illinois-Urbana Champaign for experimental assistance.en
dc.publisherSpringer Science + Business Mediaen
dc.subjectFeeding patternen
dc.subjectIntegrated omicsen
dc.subjectMetabolomicsen
dc.subjectNitrosomonas europaeaen
dc.subjectTemperatureen
dc.titleGlobal metabolomic responses of Nitrosomonas europaea 19718 to cold stress and altered ammonia feeding patternsen
dc.typeArticleen
dc.identifier.journalApplied Microbiology and Biotechnologyen
dc.contributor.institutionUniversity of Illinois at Urbana-Champaign, Urbana, United Statesen
dc.contributor.institutionUniversity of Vermont, Burlington, United Statesen
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