Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light

Handle URI:
http://hdl.handle.net/10754/598344
Title:
Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light
Authors:
Kogan, Maxim; Beke-Somfai, Tamás; Nordén, Bengt
Abstract:
Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.
Citation:
Kogan M, Beke-Somfai T, Nordén B (2011) Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light. Chem Commun 47: 7356. Available: http://dx.doi.org/10.1039/c1cc12313k.
Publisher:
Royal Society of Chemistry (RSC)
Journal:
Chemical Communications
KAUST Grant Number:
KUK-11-008-23
Issue Date:
2011
DOI:
10.1039/c1cc12313k
PubMed ID:
21637893
Type:
Article
ISSN:
1359-7345; 1364-548X
Sponsors:
This work is financed by King Abdullah University of Science and Technology (KAUST) (Grant KUK-11-008-23) and the European Research Council (ERC-2008 AdG 227700-SUMO).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorKogan, Maximen
dc.contributor.authorBeke-Somfai, Tamásen
dc.contributor.authorNordén, Bengten
dc.date.accessioned2016-02-25T13:19:05Zen
dc.date.available2016-02-25T13:19:05Zen
dc.date.issued2011en
dc.identifier.citationKogan M, Beke-Somfai T, Nordén B (2011) Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light. Chem Commun 47: 7356. Available: http://dx.doi.org/10.1039/c1cc12313k.en
dc.identifier.issn1359-7345en
dc.identifier.issn1364-548Xen
dc.identifier.pmid21637893en
dc.identifier.doi10.1039/c1cc12313ken
dc.identifier.urihttp://hdl.handle.net/10754/598344en
dc.description.abstractBicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.en
dc.description.sponsorshipThis work is financed by King Abdullah University of Science and Technology (KAUST) (Grant KUK-11-008-23) and the European Research Council (ERC-2008 AdG 227700-SUMO).en
dc.publisherRoyal Society of Chemistry (RSC)en
dc.titleFlow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized lighten
dc.typeArticleen
dc.identifier.journalChemical Communicationsen
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen
kaust.grant.numberKUK-11-008-23en

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