Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device

Handle URI:
http://hdl.handle.net/10754/598280
Title:
Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device
Authors:
Ma, W.; Liu, D.; Shagoshtasbi, H.; Shukla, A.; Nugroho, E. S.; Zohar, Y.; Lee, Y.-K.
Abstract:
Microfiltration is an important microfluidic technique suitable for enrichment and isolation of cells. However, cell lysing could occur due to hydrodynamic damage that may be detrimental for medical diagnostics. Therefore, we conducted a systematic study of hydrodynamic cell lysing in a high-throughput Circular Multi-Channel Microfiltration (CMCM) device integrated with a polycarbonate membrane. HeLa cells (cervical cancer cells) were driven into the CMCM at different flow rates. The viability of the cells in the CMCM was examined by fluorescence microscopy using Acridine Orange (AO)/Ethidium Bromide (EB) as a marker for viable/dead cells. A simple analytical cell viability model was derived and a 3D numerical model was constructed to examine the correlation of between cell lysing and applied shear stress under varying flow rate and Reynolds number. The measured cell viability as a function of the shear stress was consistent with theoretical and numerical predictions when accounting for cell size distribution. © 2013 IEEE.
Citation:
Ma W, Liu D, Shagoshtasbi H, Shukla A, Nugroho ES, et al. (2013) Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device. The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Available: http://dx.doi.org/10.1109/nems.2013.6559761.
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems
KAUST Grant Number:
SA-C0040/UK-C0016
Issue Date:
Apr-2013
DOI:
10.1109/nems.2013.6559761
Type:
Conference Paper
Sponsors:
This research was partially supported by a grant from School of Engineering, HKUST, a grant from NSFC, China (No. 81171418) and by an award from the King Abdullah University of Science and Technology (KAUST Award No. SA-C0040/UK-C0016).
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorMa, W.en
dc.contributor.authorLiu, D.en
dc.contributor.authorShagoshtasbi, H.en
dc.contributor.authorShukla, A.en
dc.contributor.authorNugroho, E. S.en
dc.contributor.authorZohar, Y.en
dc.contributor.authorLee, Y.-K.en
dc.date.accessioned2016-02-25T13:17:54Zen
dc.date.available2016-02-25T13:17:54Zen
dc.date.issued2013-04en
dc.identifier.citationMa W, Liu D, Shagoshtasbi H, Shukla A, Nugroho ES, et al. (2013) Experimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration device. The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Available: http://dx.doi.org/10.1109/nems.2013.6559761.en
dc.identifier.doi10.1109/nems.2013.6559761en
dc.identifier.urihttp://hdl.handle.net/10754/598280en
dc.description.abstractMicrofiltration is an important microfluidic technique suitable for enrichment and isolation of cells. However, cell lysing could occur due to hydrodynamic damage that may be detrimental for medical diagnostics. Therefore, we conducted a systematic study of hydrodynamic cell lysing in a high-throughput Circular Multi-Channel Microfiltration (CMCM) device integrated with a polycarbonate membrane. HeLa cells (cervical cancer cells) were driven into the CMCM at different flow rates. The viability of the cells in the CMCM was examined by fluorescence microscopy using Acridine Orange (AO)/Ethidium Bromide (EB) as a marker for viable/dead cells. A simple analytical cell viability model was derived and a 3D numerical model was constructed to examine the correlation of between cell lysing and applied shear stress under varying flow rate and Reynolds number. The measured cell viability as a function of the shear stress was consistent with theoretical and numerical predictions when accounting for cell size distribution. © 2013 IEEE.en
dc.description.sponsorshipThis research was partially supported by a grant from School of Engineering, HKUST, a grant from NSFC, China (No. 81171418) and by an award from the King Abdullah University of Science and Technology (KAUST Award No. SA-C0040/UK-C0016).en
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.subjectcell lysingen
dc.subjectcell viabilityen
dc.subjectenrichmenten
dc.subjecthydrodynamic shearen
dc.subjectisolationen
dc.subjectmicrofiltrationen
dc.subjectmicrofluidicsen
dc.titleExperimental and theoretical study of hydrodynamic cell lysing of cancer cells in a high-throughput Circular Multi-Channel Microfiltration deviceen
dc.typeConference Paperen
dc.identifier.journalThe 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systemsen
dc.contributor.institutionHong Kong University of Science and Technology, Hong Kong, Chinaen
dc.contributor.institutionGuangzhou First Municipal People's Hospital, Guangzhou, Chinaen
dc.contributor.institutionUniversity of Arizona, Tucson, United Statesen
kaust.grant.numberSA-C0040/UK-C0016en
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