Enhanced start-up of anaerobic facultatively autotrophic biocathodes in bioelectrochemical systems

Handle URI:
http://hdl.handle.net/10754/598190
Title:
Enhanced start-up of anaerobic facultatively autotrophic biocathodes in bioelectrochemical systems
Authors:
Zaybak, Zehra; Pisciotta, John M.; Tokash, Justin C.; Logan, Bruce E.
Abstract:
Biocathodes in bioelectrochemical systems (BESs) can be used to convert CO2 into diverse organic compounds through a process called microbial electrosynthesis. Unfortunately, start-up of anaerobic biocathodes in BESs is a difficult and time consuming process. Here, a pre-enrichment method was developed to improve start-up of anaerobic facultatively autotrophic biocathodes capable of using cathodes as the electron donor (electrotrophs) and CO2 as the electron acceptor. Anaerobic enrichment of bacteria from freshwater bog sediment samples was first performed in batch cultures fed with glucose and then used to inoculate BES cathode chambers set at -0.4V (versus a standard hydrogen electrode; SHE). After two weeks of heterotrophic operation of BESs, CO2 was provided as the sole electron acceptor and carbon source. Consumption of electrons from cathodes increased gradually and was sustained for about two months in concert with a significant decrease in cathode chamber headspace CO2. The maximum current density consumed was -34±4mA/m2. Biosynthesis resulted in organic compounds that included butanol, ethanol, acetate, propionate, butyrate, and hydrogen gas. Bacterial community analyses based on 16S rRNA gene clone libraries revealed Trichococcus palustris DSM 9172 (99% sequence identity) as the prevailing species in biocathode communities, followed by Oscillibacter sp. and Clostridium sp. Isolates from autotrophic cultivation were most closely related to Clostridium propionicum (99% sequence identity; ZZ16), Clostridium celerecrescens (98-99%; ZZ22, ZZ23), Desulfotomaculum sp. (97%; ZZ21), and Tissierella sp. (98%; ZZ25). This pre-enrichment procedure enables simplified start-up of anaerobic biocathodes for applications such as electrofuel production by facultatively autotrophic electrotrophs. © 2013 Elsevier B.V.
Citation:
Zaybak Z, Pisciotta JM, Tokash JC, Logan BE (2013) Enhanced start-up of anaerobic facultatively autotrophic biocathodes in bioelectrochemical systems. Journal of Biotechnology 168: 478–485. Available: http://dx.doi.org/10.1016/j.jbiotec.2013.10.001.
Publisher:
Elsevier BV
Journal:
Journal of Biotechnology
KAUST Grant Number:
KUS-I1-003-13
Issue Date:
Dec-2013
DOI:
10.1016/j.jbiotec.2013.10.001
PubMed ID:
24126154
Type:
Article
ISSN:
0168-1656
Sponsors:
The authors would like to thank Xiuping Zhu for her assistance with Shimadzu GC and HPLC analyses, and acknowledge the King Abdullah University of Science and Technology (KUS-I1-003-13) for funding this project.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorZaybak, Zehraen
dc.contributor.authorPisciotta, John M.en
dc.contributor.authorTokash, Justin C.en
dc.contributor.authorLogan, Bruce E.en
dc.date.accessioned2016-02-25T13:14:23Zen
dc.date.available2016-02-25T13:14:23Zen
dc.date.issued2013-12en
dc.identifier.citationZaybak Z, Pisciotta JM, Tokash JC, Logan BE (2013) Enhanced start-up of anaerobic facultatively autotrophic biocathodes in bioelectrochemical systems. Journal of Biotechnology 168: 478–485. Available: http://dx.doi.org/10.1016/j.jbiotec.2013.10.001.en
dc.identifier.issn0168-1656en
dc.identifier.pmid24126154en
dc.identifier.doi10.1016/j.jbiotec.2013.10.001en
dc.identifier.urihttp://hdl.handle.net/10754/598190en
dc.description.abstractBiocathodes in bioelectrochemical systems (BESs) can be used to convert CO2 into diverse organic compounds through a process called microbial electrosynthesis. Unfortunately, start-up of anaerobic biocathodes in BESs is a difficult and time consuming process. Here, a pre-enrichment method was developed to improve start-up of anaerobic facultatively autotrophic biocathodes capable of using cathodes as the electron donor (electrotrophs) and CO2 as the electron acceptor. Anaerobic enrichment of bacteria from freshwater bog sediment samples was first performed in batch cultures fed with glucose and then used to inoculate BES cathode chambers set at -0.4V (versus a standard hydrogen electrode; SHE). After two weeks of heterotrophic operation of BESs, CO2 was provided as the sole electron acceptor and carbon source. Consumption of electrons from cathodes increased gradually and was sustained for about two months in concert with a significant decrease in cathode chamber headspace CO2. The maximum current density consumed was -34±4mA/m2. Biosynthesis resulted in organic compounds that included butanol, ethanol, acetate, propionate, butyrate, and hydrogen gas. Bacterial community analyses based on 16S rRNA gene clone libraries revealed Trichococcus palustris DSM 9172 (99% sequence identity) as the prevailing species in biocathode communities, followed by Oscillibacter sp. and Clostridium sp. Isolates from autotrophic cultivation were most closely related to Clostridium propionicum (99% sequence identity; ZZ16), Clostridium celerecrescens (98-99%; ZZ22, ZZ23), Desulfotomaculum sp. (97%; ZZ21), and Tissierella sp. (98%; ZZ25). This pre-enrichment procedure enables simplified start-up of anaerobic biocathodes for applications such as electrofuel production by facultatively autotrophic electrotrophs. © 2013 Elsevier B.V.en
dc.description.sponsorshipThe authors would like to thank Xiuping Zhu for her assistance with Shimadzu GC and HPLC analyses, and acknowledge the King Abdullah University of Science and Technology (KUS-I1-003-13) for funding this project.en
dc.publisherElsevier BVen
dc.subjectBiocathodeen
dc.subjectBioelectrochemical systemen
dc.subjectBiofuelen
dc.subjectElectrofuelen
dc.subjectElectrotrophen
dc.titleEnhanced start-up of anaerobic facultatively autotrophic biocathodes in bioelectrochemical systemsen
dc.typeArticleen
dc.identifier.journalJournal of Biotechnologyen
dc.contributor.institutionPennsylvania State University, State College, United Statesen
dc.contributor.institutionWest Chester University, West Chester, United Statesen
kaust.grant.numberKUS-I1-003-13en

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