Enhanced hydrogen and 1,3-propanediol production from glycerol by fermentation using mixed cultures

Handle URI:
http://hdl.handle.net/10754/598180
Title:
Enhanced hydrogen and 1,3-propanediol production from glycerol by fermentation using mixed cultures
Authors:
Selembo, Priscilla A.; Perez, Joe M.; Lloyd, Wallis A.; Logan, Bruce E.
Abstract:
The conversion of glycerol into high value products, such as hydrogen gas and 1,3-propanediol (PD), was examined using anaerobic fermentation with heat-treated mixed cultures. Glycerol fermentation produced 0.28 mol-H 2/mol-glycerol (72 mL-H2/g-COD) and 0.69 mol-PD/mol-glycerol. Glucose fermentation using the same mixed cultures produced more hydrogen gas (1.06 mol-H2/mol-glucose) but no PD. Changing the source of inoculum affected gas production likely due to prior acclimation of bacteria to this type of substrate. Fermentation of the glycerol produced from biodiesel fuel production (70% glycerol content) produced 0.31 mol-H 2/mol-glycerol (43 mL H2/g-COD) and 0.59 mol-PD/mol-glycerol. These are the highest yields yet reported for both hydrogen and 1,3-propanediol production from pure glycerol and the glycerol byproduct from biodiesel fuel production by fermentation using mixed cultures. These results demonstrate that production of biodiesel can be combined with production of hydrogen and 1,3-propanediol for maximum utilization of resources and minimization of waste. © 2009 Wiley Periodicals, Inc.
Citation:
Selembo PA, Perez JM, Lloyd WA, Logan BE (2009) Enhanced hydrogen and 1,3-propanediol production from glycerol by fermentation using mixed cultures. Biotechnology and Bioengineering 104: 1098–1106. Available: http://dx.doi.org/10.1002/bit.22487.
Publisher:
Wiley-Blackwell
Journal:
Biotechnology and Bioengineering
Issue Date:
15-Dec-2009
DOI:
10.1002/bit.22487
PubMed ID:
19623563
Type:
Article
ISSN:
0006-3592; 1097-0290
Sponsors:
The authors thank S. Cheng and D. Jones for assistance with experiments and analysis, and Nittany Biodiesel for providing B-glycerol samples. Special thanks to Dr. Pat Cirino and Jonathan Chin for help with HPLC analysis. This research was supported in part by the Global Research Partnership (GRP) from KAUST University, the General Electric First-Year Faculty for the Future Fellowship and the Arthur and Elizabeth Rose Memorial Fellowship.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorSelembo, Priscilla A.en
dc.contributor.authorPerez, Joe M.en
dc.contributor.authorLloyd, Wallis A.en
dc.contributor.authorLogan, Bruce E.en
dc.date.accessioned2016-02-25T13:14:12Zen
dc.date.available2016-02-25T13:14:12Zen
dc.date.issued2009-12-15en
dc.identifier.citationSelembo PA, Perez JM, Lloyd WA, Logan BE (2009) Enhanced hydrogen and 1,3-propanediol production from glycerol by fermentation using mixed cultures. Biotechnology and Bioengineering 104: 1098–1106. Available: http://dx.doi.org/10.1002/bit.22487.en
dc.identifier.issn0006-3592en
dc.identifier.issn1097-0290en
dc.identifier.pmid19623563en
dc.identifier.doi10.1002/bit.22487en
dc.identifier.urihttp://hdl.handle.net/10754/598180en
dc.description.abstractThe conversion of glycerol into high value products, such as hydrogen gas and 1,3-propanediol (PD), was examined using anaerobic fermentation with heat-treated mixed cultures. Glycerol fermentation produced 0.28 mol-H 2/mol-glycerol (72 mL-H2/g-COD) and 0.69 mol-PD/mol-glycerol. Glucose fermentation using the same mixed cultures produced more hydrogen gas (1.06 mol-H2/mol-glucose) but no PD. Changing the source of inoculum affected gas production likely due to prior acclimation of bacteria to this type of substrate. Fermentation of the glycerol produced from biodiesel fuel production (70% glycerol content) produced 0.31 mol-H 2/mol-glycerol (43 mL H2/g-COD) and 0.59 mol-PD/mol-glycerol. These are the highest yields yet reported for both hydrogen and 1,3-propanediol production from pure glycerol and the glycerol byproduct from biodiesel fuel production by fermentation using mixed cultures. These results demonstrate that production of biodiesel can be combined with production of hydrogen and 1,3-propanediol for maximum utilization of resources and minimization of waste. © 2009 Wiley Periodicals, Inc.en
dc.description.sponsorshipThe authors thank S. Cheng and D. Jones for assistance with experiments and analysis, and Nittany Biodiesel for providing B-glycerol samples. Special thanks to Dr. Pat Cirino and Jonathan Chin for help with HPLC analysis. This research was supported in part by the Global Research Partnership (GRP) from KAUST University, the General Electric First-Year Faculty for the Future Fellowship and the Arthur and Elizabeth Rose Memorial Fellowship.en
dc.publisherWiley-Blackwellen
dc.subject1,3-propanediolen
dc.subjectBiodieselen
dc.subjectFermentationen
dc.subjectGlycerolen
dc.subjectHydrogenen
dc.subjectPretreatmenten
dc.subjectRaw glycerolen
dc.titleEnhanced hydrogen and 1,3-propanediol production from glycerol by fermentation using mixed culturesen
dc.typeArticleen
dc.identifier.journalBiotechnology and Bioengineeringen
dc.contributor.institutionPennsylvania State University, State College, United Statesen

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